166,224 results

1. pCW empty vector

   Plasmid

   #184708

   Purpose

   Lentiviral empty vector for doxycycline-inducible expression

   Depositor

   Alessia Ciarrocchi, Gloria Manzotti

   Article

   Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

   Plasmid

   #105543

   Purpose

   AAV expression of EGFP from cTNT promoter

   Depositor

   James M. Wilson

   Has Service

   AAV9

   Insert

   cTNT-PI-EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   cTNT

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFGET19_Ulp1

   Plasmid

   #64697

   Purpose

   H6-Ulp1 from pHYRS52 into pET-28b(+)

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Insert

   ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Promoter

   T7

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pH6-MBP-TEV-BirA

   Plasmid

   #179694

   Purpose

   Expresses His6 tagged MBP-BirA fusion protein

   Depositor

   Mark Arbing

   Insert

   BirA
   

   Tags

   His6-MBP

   Expression

   Bacterial

   Available Since

   Jan. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-HIF1alpha-pcDNA3

   Plasmid

   #18949

   Depositor

   William Kaelin

   Article

   Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-GFP11-Clathrin light chain

   Plasmid

   #70217

   Purpose

   Expresses GFP11-clathrin light chain in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11-Clathrin light chain (CLTA Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpySwitch

   Plasmid

   #184225

   Purpose

   Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpySwitch
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E77A mutation prevents isopeptide bond formation,…

   Promoter

   T7

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROP-seq-opti

   Plasmid

   #106280

   Purpose

   A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   EF1a-Puro-WPRE-hU6-gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Flag mTOR wt

   Plasmid

   #26603

   Purpose

   Mammalian expression of human flag-tagged mTor

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   mTOR (MTOR Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Oct. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Expression

    Insect

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2904

    Plasmid

    #143388

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DACH1 (DACH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSIN-PAmCherry-KFERQ-NE

    Plasmid

    #102365

    Purpose

    Lentiviral overexpression of PA-mCherry-KFERQ fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    KFERQ peptide
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-HP1alpha

    Plasmid

    #17652

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 alpha (CBX5 Human)
    

    Tags

    GFP and His

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAVEC2_C-mTurquoise2

    Plasmid

    #231294

    Purpose

    For transiently expressing fusion proteins with C terminally tagged mTurquoise2 in plants (includes p19 silencing suppressor)

    Depositor

    Jacob Brunkard

    Article

    Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

    Type

    Empty backbone

    Tags

    mTurquoise2

    Expression

    Plant

    Mutation

    N/A

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAVEC2_C-mEGFP

    Plasmid

    #231295

    Purpose

    For transiently expressing fusion proteins with C terminally tagged mEGFP in plants (includes p19 silencing suppressor)

    Depositor

    Jacob Brunkard

    Article

    Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Plant

    Mutation

    N/A

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCHAC-mt-mKeima

    Plasmid

    #72342

    Purpose

    retrovirus construct for stable expressing mt-mKeima

    Depositor

    Richard Youle

    Article

    Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

    Insert

    mt-mKeima
    

    Use

    Retroviral

    Available Since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ABE8.20-SpRY

    Plasmid

    #226859

    Purpose

    Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSmartBAC-S

    Plasmid

    #107268

    Purpose

    Broad host range bacterial artificial chromosome vector

    Depositor

    Ron Godiska, Mark Liles

    Article

    Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HIV-1 Gag-mCherry

    Plasmid

    #85390

    Purpose

    Visualization marker for viral core to measure cellular uptake

    Depositor

    Gregory Melikyan

    Article

    Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

    Insert

    HIV-1 gag
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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