We narrowed to 12,629 results for: 109
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Plasmid#175555PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.DepositorInsertspCas9-nuclease and sgRNA against mouse NANOG STOP Codon
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct
Plasmid#175554PurposeTargeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.DepositorInsertNANOG
UseMouse TargetingTagsFKBPV-HA2-P2A-eGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-Cox3.1-1397C-AD.
Plasmid#212953PurposeExpressesTALED_Right-Cox3.1-1397C-AD in mammalian cellsDepositorInsertTALED_Right-Cox3.1-1397C-AD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-Cox3.1-1397N.
Plasmid#212950PurposeExpresses TALED_Left-Cox3.1-1397N. in mammalian cellsDepositorInsertTALED_Left-Cox3.1-1397N
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-mouse-ATP6-1397N.
Plasmid#212951PurposeExpresses TALED_Left-mouse-ATP6-1397N in mammalian cellsDepositorInsertTALED_Left-mouse-ATP6-1397N (ATP6 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-ND6-1397N
Plasmid#212952PurposeExpresses TALED_Left-ND6-1397N in mammalian cellsDepositorInsertTALED_Left-ND6-1397N
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-ND6-1397C-AD
Plasmid#212956PurposeExpresses TALED_Right-ND6-1397C-AD in mammalian cellsDepositorInsertTALED_Right-ND6-1397C-AD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-I560S-mDPYD
Plasmid#61615PurposeThe mouse Dpyd was used to rescue the human DPYD hairpin affect on EMTDepositorInsertDihydropyrimidine dehydrogenase (Dpyd Mouse)
UseTagsExpressionMammalianMutationChanged Isoleucine at position 560 to SerinePromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_5SA-PolyA
Plasmid#112285PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)DepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with 5 Serines (61, 109, 127, 1…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct
Plasmid#175549PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.DepositorInsertWAPL (Wapl Mouse)
UseMouse TargetingTagsAID[71-114]-eGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNQL004-SOX2-FKBPV-HA2-P2A-mCherry targeting construct
Plasmid#175552PurposeTargeting vector to introduce an FKBPV-HA2-P2A-mCherry cassette at the mouse SOX2 locus. Degradation Tag (dTAG) system.DepositorInsertSOX2 (Sox2 Mouse)
UseMouse TargetingTagsFKBPV-HA2-P2A-mCherryExpressionMammalianMutationnonePromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-mouse-ATP6-1397C-AD(V28R)
Plasmid#212955PurposeExpresses TALED_Right-mouse-ATP6-1397C-AD(V28R) in mammalian cellsDepositorInsertRight-mouse-ATP6-1397C-AD(V28R) (ATP6 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A
Plasmid#212949PurposeExpresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian cellsDepositorInsertTALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A (mt-Rnr1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-mouse-ATP6-1397C-AD
Plasmid#212954PurposeExpresses TALED_Right-mouse-ATP6-1397C-AD in mammalian cellsDepositorInsertTALED_Right-mouse-ATP6-1397C-AD (ATP6 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Kss1
Plasmid#52682PurposeExpression of Kss1 in bacteriaDepositorInsertKSS1 (KSS1 Budding Yeast)
UseTagsHis6 and T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#156452PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTGDepositorInsertRAD21, AID (Rad21 Mouse)
UseMouse TargetingTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK528
Plasmid#71625PurposeProduces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Met105>Ser/Ile109>Trp mutant (TdPurE2-M105S,I109W)DepositorInsertphosphoribosylaminoimidazole carboxylase
UseTagsExpressionBacterialMutationchanges methionine-105 to serine, isoleucine-109 …PromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Ste7-EE
Plasmid#52681Purposeexpression of constitutively active Ste7 kinase in bacteriaDepositorInsertSte7 (STE7 Budding Yeast)
UseTagsGST and MycExpressionBacterialMutationS359E/T363E constitutively activePromotertacAvailable sinceApril 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ITSN2_ADCY3
Plasmid#205832PurposeExpress mEGFP-tagged fusion protein, ITSN2_ADCY3 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SFFV_V5-CHIC2-C6S_Tomato
Plasmid#238128PurposeExpresses V5-tagged CHIC2 (with C6S mutation) with Tomato selectable marker.DepositorInsertV5-tagged CHIC2 with C6S mutation
UseTagsV5ExpressionMammalianMutationChanged cysteines from amino acids 102-109 to ser…PromoterSFFVAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCI-MOR250-1
Plasmid#22366DepositorInsertMOR250-1 (Olfr109 Mouse)
UseTagsRhoExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET20b-pIgR D1-H6
Plasmid#12530DepositorInserthuman polymeric immunoglobulin receptor (PIGR Human)
UseTagsHisExpressionBacterialMutationLys 1-Val 109PromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-pIgR D1-H6
Plasmid#12531DepositorInserthuman polymeric immunoglobulin receptor (PIGR Human)
UseTags6xHisExpressionInsectMutationLys 1 - Val 109PromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIVT-AIDm-EGFP
Plasmid#182044Purposein vitro transcription of AIDm-EGFPDepositorInsertAIDm-EGFP
UseIn vitro transcriptionTagsEGFPExpressionMutationPromoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
HMEKc-srcM
Plasmid#1107DepositorInsertc-src
UseTagsExpressionYeastMutationMET3 promoter inserted in EcoRI and BamHI of pRS3…PromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-FOXC1-MUT
Plasmid#194567PurposeMammalian Expression of mEGFP-FOXC1 Mutant (Q200Rfs*109) Fusion ProteinDepositorInsertFOXC1 (FOXC1 Human)
UseTagsmEGFPExpressionMammalianMutationQ200Rfs*109PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Integrin-β6 Headpiece I270C
Plasmid#159638PurposeContains integrin beta 6 residues 1–474 with I270C mutationDepositorInsertIntegrin beta 6 headpiece I270C
UseTags6x HisExpressionMammalianMutationI270CPromoterAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP112 (pRS406-GFP-ADH1t)
Plasmid#106472PurposeVector for creating C-terminal GFP fusion proteins in yeast.DepositorTypeEmpty backboneUseTagsGFPExpressionBacterial and YeastMutationPromoterNoneAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAUC40
Plasmid#71298PurposeSuicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
Integrin-αv Headpiece M400GC
Plasmid#159637PurposeContains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400DepositorInsertalpha v headpiece M400GC
UseTags6x His and Strep IIExpressionMammalianMutationM400CPromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03
Plasmid#158793PurposeExpresses influenza A virus tetrameric neuraminidase fused to a sfGFPDepositorInsertNeuraminidase A/NL/109/03
UseTagsTwinStrep_sfGFP_tetrabrachionExpressionMammalianMutationAA61-469PromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz75_CB_operon_CsoS2_N3-N4
Plasmid#140857PurposeSame as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-109 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pclbw-eGFPomp25
Plasmid#58424PurposeMammalian expression of GFP targeted to the mitochondrial outer membraneDepositorInserteGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25PromoterCMV/b-actinAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)
Plasmid#106464PurposeExpresses Prc1 with a C-terminal GFP tag in yeast cellsDepositorInsertPRC1
UseTagsGFPExpressionBacterial and YeastMutationPromoterPRC1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pclbw-PAGFPomp25
Plasmid#58427PurposeMammalian expression of photoactivatable GFP targeted to the mitochondrial outer membraneDepositorInsertpaGFP-omp25 (Synj2bp Mouse)
UseRetroviralTagsPAGFPExpressionMammalianMutationcontains aa's 109-145 of mouse omp25Promotercmv-bactinAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKP115 (pRS406-YBR139Wp-YBR139W-GFP-ADH1t)
Plasmid#106465PurposeExpresses Atg42/Ybr139w with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W
UseTagsGFPExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP136 (pRS406-PRC1p-PRC1-PA-ADH1t)
Plasmid#106471PurposeExpresses Prc1 with a C-terminal PA tag in yeast cellsDepositorInsertPRC1
UseTagsPAExpressionBacterial and YeastMutationPromoterPRC1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106461PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106470PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSN65
Plasmid#100123Purposeexpresses putative SOX2 DBD with a V5 tagDepositorInsertmCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]
Plasmid#106462PurposeExpresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W(N163,242Q)
UseTagsPAExpressionBacterial and YeastMutationchanged Asparagines at positions 163 and 242 to G…PromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP129 [pRS406-YBR139Wp-YBR139W(S219A)-GFP-ADH1t]
Plasmid#106466PurposeExpresses Atg42/Ybr139w(S219A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(S219A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Serine 219 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]
Plasmid#106467PurposeExpresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(H474A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Histidine 474 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]
Plasmid#106468PurposeExpresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(D415A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Aspartate 415 to AlaninePromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUHD 10.3-human MIZ1 delta POZ
Plasmid#74160PurposeTet inducible expression of MIZ1 delta POZDepositorInsertMIZ1 delta POZ (ZBTB17 Human)
UseTagsExpressionMammalianMutationdelta POZ domain (first 109 aa)PromoterCMV2Available sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]
Plasmid#106469PurposeExpresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(S219,D415,H474A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Serine 219, Aspartate 415 and Histidine 4…PromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-1
Plasmid#72680PurposeSame as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationlower strength RBS before mutL E32K allele than i…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLM-AMA15.0 Cas9
Plasmid#138944PurposeFungal AMA1 plasmid with sp-Cas9, ribozymes based "plug-and-play" sgRNA transcription unit, Terbinafine and Phleomycin selection markersDepositorInsertp40S:sp_Cas9_2xNLS; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers
UseCRISPR and Synthetic Biology; Ama1 autonomously r…TagsNLSExpressionMutationPromoterp40S (AN0465) Aspergillus nidulansAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only