Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 6,501 results for: nls

Clear keywords
Showing: 501 - 520 of 6501 results

  2. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

    Plasmid
    #155305
    Purpose
    Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.
    Depositor
    Stanley Qi
    Publication
    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do
    Insert
    mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
    Use
    Lentiviral
    Tags
    2xNLS-3xFLAG
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    155305-plasmid-map-sequence-id-306279

    Enlarge

  3. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    58477-plasmid-map-sequence-id-90386

    Enlarge

  5. pCMV-PE6d

    Plasmid
    #207854
    Purpose
    Mammalian expression of PE6d prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6d
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    207854-plasmid-map-sequence-id-445303

    Enlarge

  6. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    114072-plasmid-map-sequence-id-220522

    Enlarge

  16. pNJP

    Plasmid
    #115494
    Purpose
    Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells
    Depositor
    Kazuhiro Aoki
    Publication
    Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Synthetic, Human, Mouse)
    Tags
    iRFP, mCherry, and mKO
    Expression
    Mammalian
    Mutation
    mCherry S227F, mCherry G229R, mKO V211G
    Promoter
    CAG
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    115494-plasmid-map-sequence-id-231521

    Enlarge

  17. pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS

    Plasmid
    #241915
    Purpose
    Zebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker
    Depositor
    Balázs Enyedi
    Publication
    Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116
    Insert
    QUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS
    Use
    Zebrafish tol2 transgenesis
    Promoter
    QUASM1
    Available since
    Oct. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241915-plasmid-map-sequence-id-487161

    Enlarge

  18. Puro-Cas9 donor

    Plasmid
    #58409
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus
    Depositor
    Danwei Huangfu
    Publication
    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    58409-plasmid-map-sequence-id-392399

    Enlarge

Showing: 501 - 520 of 6501 results