We narrowed to 13,971 results for: RING

1. pJL NarX Myc

   Plasmid

   #225651

   Purpose

   Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NarX (narX E. coli)
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-SEP-GluR1

   Plasmid

   #24000

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR1 (Gria1 Rat)
   

   Tags

   GluR1 signal peptide (aa1-22) and SEP (supereclip…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDL17

   Plasmid

   #106483

   Purpose

   pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   exo
   lacI
   rhaR
   rhaS
   

   Expression

   Bacterial

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB076i7

   Plasmid

   #79201

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209663

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Mutation

   S29W.A95K

   Promoter

   hSyn

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-P4:gs:nls:CIBN

   Plasmid

   #135986

   Purpose

   Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Insert

   P4 coiled-coil fused to the CIBN domain
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TetO-FUW-cMYC

   Plasmid

   #20324

   Depositor

   Rudolf Jaenisch

   Article

   Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

   Insert

   cMYC (Myc Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1(+)-NEMOc

   Plasmid

   #189933

   Purpose

   Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

   Depositor

   Yubin Zhou

   Article

   Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

   Insert

   NEMOc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-Cdc42-2G

   Plasmid

   #68813

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Lentiviral

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB107a

    Plasmid

    #79218

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAB076i6

    Plasmid

    #79200

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (no operator); Ppro1

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL1749 (pDonor, R*MmeI)

    Plasmid

    #160733

    Purpose

    Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST donor DNA
    

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-HA-Ubiquitin-K11

    Plasmid

    #22901

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K11 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-CRY2Clust-FUSN

    Plasmid

    #200967

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSN
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1 human cofilin WT

    Plasmid

    #50859

    Purpose

    expresses human cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFC334

    Plasmid

    #87846

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    argB
    yA specific sgRNA
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    A. nidulans gpdA promoter with Hammerhead ribozym…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-Ubiquitin-K6

    Plasmid

    #22900

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K6 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI-SEP GluR2(R)

    Plasmid

    #24001

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR2 (Gria2 Rat)
    

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMOD_B2520

    Plasmid

    #91077

    Purpose

    Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    OsU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pQCi-mB2m-sgRNA

    Plasmid

    #154090

    Purpose

    pQCi backbone with sgRNA targeting murine Beta-2-microglobulin

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA targeting murine B2M locus
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSpCas9n(BB)-2A-Puro (PX462)

    Plasmid

    #48141

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pDN77

    Plasmid

    #61347

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Tags

    GS linker-AsLov2-biNLS2 and NES IkBa

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSNK

    Plasmid

    #135193

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. L0-I35 p35S-XVE-pEstradiol GG0-GG1

    Plasmid

    #195904

    Purpose

    Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    p35S-XVE-LexA-p35S
    

    Use

    Synthetic Biology

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV.GFAP.(cyto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209661

    Purpose

    Expression of iATPSnFR2, low affinity

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    AAV

    Mutation

    A95A.A119L

    Promoter

    GFAP

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCAT009

    Plasmid

    #171635

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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29. pWN_U6-TRAC-miniGag-Cas9

    Plasmid

    #228959

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV_nLightG

    Plasmid

    #217656

    Purpose

    Expresses norepinephrine indicator nLightG under mammalian promoter

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    nLightG
    

    Expression

    Mammalian

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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