161,805 results

1. pCMV flag ERR alpha

   Plasmid

   #10975

   Depositor

   Toren Finkel

   Article

   Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

   Insert

   ERR alpha (ESRRA Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMGIB-3xFLAG-dyskerin

   Plasmid

   #53607

   Purpose

   Retroviral expression of human dyskerin

   Depositor

   Steven Artandi

   Article

   Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

   Insert

   dyskerin (DKC1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCDNA3.1-spikeRBD::∆ACE2 SURF

   Plasmid

   #206957

   Purpose

   Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

   Depositor

   Xiaokun Shu

   Article

   Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

   Inserts

   sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
   ACE2 (ACE2 Human)
   

   Use

   Tags

   cSURF, mCherry, and nSURF

   Expression

   Mammalian

   Mutation

   17-615 and RBD (319-541)

   Promoter

   CMV

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

   Plasmid

   #132393

   Purpose

   Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Proline 301 to Leucine (P301L)

   Promoter

   TREtight

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Puro-mStayGold-LMNB1

   Plasmid

   #227329

   Purpose

   Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Anti-GFP [N86/34R]

   Plasmid

   #220419

   Purpose

   Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSDM052

   Plasmid

   #194341

   Purpose

   pUC mini-Tn7 vector expressing LseCas13a

   Depositor

   Joseph Bondy-Denomy

   Article

   Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

   Insert

   Cas13a(Lse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPB_TRE3G_scFV-sfGFP-Ezh2FL

   Plasmid

   #235575

   Purpose

   Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 4xmts-mNeonGreen

   Plasmid

   #98876

   Purpose

   In vivo visualization of mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   4xmts
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

    Plasmid

    #224006

    Purpose

    AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2XMyc-LRRK2-3XKD

    Plasmid

    #25366

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. STING-miRFP680

    Plasmid

    #200940

    Purpose

    STING fused with miRFP680 for live cell imaging

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    STING (STING1 Human)
    miRFP680
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-HA-rat Nrxn1beta AS4(-)

    Plasmid

    #58267

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn1beta AS4(-) (Nrxn1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    see comments

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-Tet-GBP1-C589A

    Plasmid

    #138090

    Purpose

    Lentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)

    Depositor

    Eva Frickel

    Article

    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

    Insert

    human GBP1 (GBP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    cysteine 589 to alanine (C589A)

    Promoter

    minimal CMV promoter with heptamerized tet‐operat…

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-elavl3

    Plasmid

    #82400

    Purpose

    Gateway-compatible zebrafish elavl3 promoter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    elavl3 promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1a-Ronin-FLAG-IRES-Neo

    Plasmid

    #28020

    Depositor

    Thomas Zwaka

    Article

    Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

    Insert

    Ronin (THAP11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG8Ha-EWS-FLI1_Type2

    Plasmid

    #188042

    Purpose

    Expresses His8-EWS-FLI1(TypeII) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

    Insert

    EWS-FLI1 (EWSR1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3HD1RLuc2

    Plasmid

    #236276

    Purpose

    Mammalian expression of human dopamine D1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    dopamine D1 receptor (DRD1 Human)
    

    Use

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPR-Cre stuffer v4

    Plasmid

    #158033

    Purpose

    Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGCC4

    Plasmid

    #37058

    Depositor

    Hank Seifert

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV.hSynap.iGABASnFR

    Plasmid

    #112159

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Has Service

    AAV1

    Insert

    iGABASnFR
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCM18

    Plasmid

    #78565

    Purpose

    60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid

    Depositor

    James Kaper

    Article

    Morin et al FEMS Immunol Med Microbiol. 2009 Oct;57(

    Type

    Empty backbone

    Use

    Tags

    luxCDABE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. amajLime chromoprotein

    Plasmid

    #117843

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amajLime
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TFORF1708

    Plasmid

    #143970

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPP1R13L (PPP1R13L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTipi2.1-AZU_SP

    Plasmid

    #229144

    Purpose

    Allows for expression of recombinant antibodies. Includes human azurocidin signal peptide.

    Depositor

    Institute for Protein Innovation (IPI)

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pAAV-hSyn-GRAB_OT1.0

    Plasmid

    #185386

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Has Service

    AAV1 and AAV9

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET15TV-L-hlPYK WT

    Plasmid

    #187975

    Purpose

    Expresses the human liver pyruvate kinase

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    hlPYK WT
    

    Use

    Tags

    Hexahistag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPW3758 : CMVd3-HsIRE1-HaloTag

    Plasmid

    #185680

    Purpose

    Low-level transient expression of full-length HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-hPDGFRA-GFP

    Plasmid

    #22919

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-EGFR

    Plasmid

    #116731

    Purpose

    Lentiviral expression of EGFR

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPB-EF1A-mSTING

    Plasmid

    #229739

    Purpose

    Expresses mouse STING in mammalian cells. Stable expression with piggybac transposase.

    Depositor

    Shawn Ferguson

    Article

    Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

    Insert

    STING (Sting1 Mouse)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX-EZH2

    Plasmid

    #28060

    Depositor

    Mien-Chie Hung

    Article

    Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

    Plasmid

    #213024

    Purpose

    Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

    Depositor

    Oliver Weichenrieder, Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (238-1061)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7 / Lac

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLIX403-iRFP-ccdB-Puro

    Plasmid

    #158566

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA GFP10-ZIP

    Plasmid

    #182241

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP
    

    Use

    Tags

    split-GFP10 tripartite

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA 3 5' cMyc SMC1 wt

    Plasmid

    #32363

    Depositor

    Michael Kastan

    Article

    Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

    Insert

    SMC1A (SMC1A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Ecoli PancACe

    Plasmid

    #215712

    Purpose

    Expresses acetyl-CoA biosensor in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    PancACe
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pShHELIX_sgRNA_entry (CJT32)

    Plasmid

    #181782

    Purpose

    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge