We narrowed to 30,195 results for: REP

Showing: 7831 - 7860 of 30195 results

Results per page:

Sort results by:

CAG-Cas9

Plasmid

#89995

Purpose

Expresses high levels of WT Cas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Aug;23:105-108. doi:

Insert

hCas9

Tags

3xFlag, SV40 NLS, and T2A peptide

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
6xAP-1_RE::GrRenilla

Plasmid

#124534

Purpose

AP-1 GreenRenilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

AP-1GreenRenilla reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PA-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187922

Purpose

Expresses C terminal 3xFLAG tagged PA subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase acidic protein

Tags

3xFLAG

Promoter

CMV Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PB2-B/BRISBANE/60/2008

Plasmid

#187917

Purpose

Expresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 2

Tags

UNTAGGED

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330-SpCas9-HF1

Plasmid

#108301

Purpose

pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pL6puro-pmRevErbA-Venus-NLS-Pest

Plasmid

#240105

Purpose

A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein)

Depositor

Achim Kramer

Article

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-miR-133

Plasmid

#172392

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Article

Zhou et al Cell Stem Cell. 2019 Jul 3;25(1):149-164

Insert

miR-133

Use

Retroviral

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSin-NE1m

Plasmid

#172883

Purpose

Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

Depositor

J. Julius Zhu

Article

Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

Insert

NE SENSOR

Expression

Mammalian

Promoter

sp6

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
7xSMAD_RE::GrRenilla

Plasmid

#178319

Purpose

SMAD GreenRenilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

SMAD GreenRenilla Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP‐RNASEH2C

Plasmid

#108698

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2C (RNASEH2C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459V2.0-SpCas9-HF1

Plasmid

#108293

Purpose

pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
3xFLAG-SENP6

Plasmid

#42887

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Please see depositor comments

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
3xDBE_RE::Renilla

Plasmid

#124535

Purpose

Foxo Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

FOXO Renilla reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Mucin MUC1-5TR-pET15b

Plasmid

#174694

Purpose

Plasmid expressing five copies of the MUC1 tandem repeat (MUC1-5TR)

Depositor

Jean Gariepy

Article

Brokx et al Biochemistry. 2003 Dec 2;42(47):13817-25

Insert

MUC1-TR

Tags

hexahistidine (HIS) tag

Expression

Bacterial

Mutation

none

Promoter

T7 Promoter

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

Plasmid

#45579

Purpose

Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani K227M

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pETcLIC-GFP

Plasmid

#183514

Purpose

The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

Depositor

Martin Högbom

Article

Grave et al Protein Expr Purif. 2022 Oct;198:106132.

Type

Empty backbone

Tags

10x His-tag and folding reporter GFP

Expression

Bacterial

Promoter

T7 lac

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SB-SRT-Puro

Plasmid

#154888

Purpose

Sleeping Beauty self-reporting transposon with puromycin reporter

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

Sleeping beauty SRT

Use

Transposon

Expression

Mammalian

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF1C-A-RFP

Plasmid

#172431

Purpose

StrepFlex MoClo vector for cloning in E. coli and Streptomyces expression - equivalent of pTU1-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Type

Empty backbone

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIY-mETV3-Flag

Plasmid

#34579

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Retroviral

Tags

FLAG and IRES-YFP

Expression

Mammalian

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

Plasmid

#192406

Purpose

To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSF2C-A-RFP

Plasmid

#172432

Purpose

StrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Type

Empty backbone

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT42_GalL_BE-PAPAPA

Plasmid

#145035

Purpose

Expresses BE-PAPAPA in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMALp2x-SCO5461

Plasmid

#42507

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

April 22, 2013

Availability

Academic Institutions and Nonprofits only
pJT43_GalL_BE-P(AP)3

Plasmid

#145036

Purpose

Expresses BE-P(AP)3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-(AP)3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459V2.0-SpCas9-HF4

Plasmid

#108295

Purpose

pX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1‐Afu‐rnhBΔPIP

Plasmid

#108696

Purpose

For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

rnhB

Tags

GST

Expression

Bacterial

Mutation

C-terminal truncation (7aa) removing PIP box

Promoter

tac

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1‐Afu‐rnhB D101N

Plasmid

#108695

Purpose

For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutation

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

rnhB

Tags

GST

Expression

Bacterial

Mutation

D101N catalytic site mutation

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Luc-Nanog-3'UTR

Plasmid

#63893

Purpose

Luciferase reporter of mouse wildtype Nanog 3'UTR

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Expression

Mammalian

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ_NAND2_L17_S19_S11

Plasmid

#132730

Purpose

T7 RNAP-driven expression of a 2-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 19 and 11

Depositor

Alexander Green, Peng Yin

Article

Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

Insert

3WJ_NAND2_L17_S19_S11

Expression

Bacterial

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge