We narrowed to 17,589 results for: She

1. pCMV-BE4max-NRTH

   Plasmid

   #136919

   Purpose

   Expresses BE4max-NRTH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   BE4max-NRTH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-puro-tetON-shRFP

   Plasmid

   #110940

   Purpose

   Tet-inducible shRNA targeting RFP

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shRFP
   

   Use

   Lentiviral and RNAi

   Promoter

   H1/TO

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCardinal-Lysosomes-20

   Plasmid

   #56166

   Purpose

   Localization: Lysosome Membrane, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   LAMP1
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pAAV-Syn-SomArchon

   Plasmid

   #126941

   Purpose

   Expression of SomArchon under Syn promoter

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

   Insert

   SomArchon
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRARE-MBP-DEST

   Plasmid

   #84650

   Purpose

   Gateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.

   Depositor

   Neil Olszewski

   Article

   Steiner et al Plant Cell. 2012 Jan;24(1):96-108. doi:

   Type

   Empty backbone

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SYN-H-sDarken

   Plasmid

   #184797

   Purpose

   High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   H-sDarken
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TBX5-FLAG in pLX303

   Plasmid

   #42563

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   TBX5 (TBX5 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Flag-Trib3

   Plasmid

   #131157

   Purpose

   Mammalian expression of flag-tagged mouse TRIB3

   Depositor

   Karen Keeshan, Warren Pear

   Article

   Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

   Insert

   Trib3 (Trib3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA-C12orf66

   Plasmid

   #87048

   Purpose

   mammalian expression of C12orf66

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   C12orf66 (C12orf66 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL3.7m-psRaf1

    Plasmid

    #89364

    Purpose

    A lenti-viral vector expressing photoswitchable Raf1 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psRaf1 (RAF1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-human CSF-1Rwt-IRES-GFP

    Plasmid

    #115523

    Purpose

    Mammalian expression construct

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    human CSF-1 receptor (CSF1R Human)
    

    Use

    Retroviral

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRVL-6

    Plasmid

    #104584

    Purpose

    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    

    Expression

    Mammalian

    Mutation

    L234A/L235E/G237A and K322A/P331S

    Promoter

    CMV

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mNeptune2

    Plasmid

    #51309

    Purpose

    Expresses far-red fluorescent protein mNeptune2 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SYN-L-sDarken

    Plasmid

    #184798

    Purpose

    Low affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    L-sDarken
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL3.7m-psCDK5

    Plasmid

    #89365

    Purpose

    A lenti-viral vector expressing photoswitchable CDK5 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psCDK5 (CDK5 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-HA-KPTN

    Plasmid

    #87042

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. OA-986F (AAEL003877-dCas9-VPR)

    Plasmid

    #183993

    Purpose

    Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    AAEL003877-dCAS9-VPR
    

    Use

    CRISPR

    Tags

    dCas9-VPR

    Expression

    Insect

    Promoter

    AAEL003887

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUASp YFP Ras85D CA

    Plasmid

    #52247

    Purpose

    Drosophila expression of YFP-Ras85D constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEPQD0KN0750

    Plasmid

    #185627

    Purpose

    Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    lacZ
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. Dead Streptavidin-SpyCatcher

    Plasmid

    #59547

    Purpose

    pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

    Insert

    Dead-dloop-Streptavidin-SpyCatcher
    

    Tags

    N-terminally shortened SpyCatcher

    Expression

    Bacterial

    Mutation

    PolyAsp in L3/4 loop for ion-exchange

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

23. shTBX5 # 2

    Plasmid

    #42550

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

24. shTBX5 # 1

    Plasmid

    #42549

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shTBX5 (TBX5 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pRC10-EFS-huLbCpf1-2A-Blast-WPRE

    Plasmid

    #123359

    Purpose

    Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV_Actb HR donor

    Plasmid

    #97317

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5-HA-ITFG2

    Plasmid

    #87045

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223_HcRed

    Plasmid

    #25892

    Purpose

    Gateway entry vector containing HcRed.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    hcRed
    

    Use

    Gateway entry vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XE120 CAMKII-K42M 1-271-CS2+

    Plasmid

    #16734

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII-K42M 1-271 (Camk2a Rat)
    

    Use

    Xenopus expression

    Mutation

    C-terminal truncation of K42M kinase

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pC-HYG-YR

    Plasmid

    #61765

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Hygromycin
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Promoter

    trpC promoter

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDONR223-VPS35

    Plasmid

    #21689

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Vacuolar Protein Sorting 35 homolog (VPS35 Human)
    

    Use

    Gateway entry clone

    Mutation

    last nucleotide of stop codon removed for c-termi…

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pRK5 CASTOR1-Flag Cterm

    Plasmid

    #84488

    Purpose

    overexpression of CASTOR1 with Flag tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 (GATSL3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

33. p11d-tRPA-32Ala9

    Plasmid

    #102616

    Purpose

    Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).

    Depositor

    Marc Wold

    Inserts

    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    

    Expression

    Bacterial

    Mutation

    with an alanine substituted phosphorylation domain

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTrc99S-ssDsbA-MBP-4xDQNAT

    Plasmid

    #128398

    Purpose

    Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Y342H- please see depositor comments

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSOMA-NLS

    Plasmid

    #118937

    Purpose

    Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-CaMKII-SomArchon

    Plasmid

    #126942

    Purpose

    Expression of SomArchon under CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Fz4-GFP

    Plasmid

    #42197

    Depositor

    Jeremy Nathans, Robert Lefkowitz

    Article

    Chen et al Science. 2003 Sep 5;301(5638):1391-4.

    Insert

    Frizzled 4 (Fzd4 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid

    #122964

    Purpose

    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Insert

    human Chibby1 (CBY1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Promoter

    FoxJ1 and human FoxJ1

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDL14

    Plasmid

    #106901

    Purpose

    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

    Depositor

    Sergey Sineoky

    Article

    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

    Inserts

    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    

    Expression

    Bacterial

    Mutation

    Lysine 622 was replaced by Alanine

    Available Since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSOMA-NES

    Plasmid

    #118939

    Purpose

    Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge