163,570 results

1. pcDNA3.2 TDP-43 NLS1 YFP

   Plasmid

   #84912

   Purpose

   Mammalian expresion of TDP-43 NLS1 YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   K82A, R83A, K84A

   Promoter

   CMV

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-EF1afull-H2B-mCherry-P2A-Fluc

   Plasmid

   #207253

   Purpose

   Lentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoter

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   H2B-mCherry-P2A-Fluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha_full

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV BiP-Myc-KDEL-wt

   Plasmid

   #27164

   Purpose

   Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

   Depositor

   Ron Prywes

   Article

   Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

   Insert

   BiP (HSPA5 Human)
   

   Use

   Tags

   KDEL and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor_ShCAST_kanR

   Plasmid

   #127924

   Purpose

   Kanamycin resistance donor for ShCAST

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG:GPI-GFP

   Plasmid

   #32601

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Rhee et al Genesis. 2006 Apr;44(4):202-18.

   Insert

   GPI-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Red cGull

   Plasmid

   #191853

   Purpose

   Expresses biosensor for cGMP in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

   Insert

   Red cGull
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ALKBH5-dCas9

   Plasmid

   #134783

   Purpose

   Expression fusion protein ALKBH5-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   ALKBH5 and inactive dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2207

   Plasmid

   #143354

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB3L2 (CREB3L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGIP

   Plasmid

   #26777

   Purpose

   Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   EF1a-GFP-IRES-Puro (gfp )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2882

    Plasmid

    #143653

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNL [NlucP/ISRE/Hygro] Vector

    Plasmid

    #236834

    Purpose

    Express ISRE response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ISRE
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Expression

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pDEST53-LRRK2-WT

    Plasmid

    #25044

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. MS2-epegRNA-Dnmt1

    Plasmid

    #211372

    Purpose

    Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MS2-epegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. FH-TET3-pEF

    Plasmid

    #49446

    Purpose

    This plasmid expresses full-length human TET3.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    Tet methylcytosine dioxygenase 3 (TET3 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTP396

    Plasmid

    #149336

    Purpose

    Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

    Depositor

    Rebecca Voorhees

    Article

    Pleiner et al Science. 2020 May 21. pii: science.abb50

    Insert

    anti-GFP nanobody
    

    Use

    Tags

    Biotin acceptor peptide (Avi-tag), His14, and SUM…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti_dCas9-KRAB-MeCP2

    Plasmid

    #122205

    Purpose

    Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Lentiviral

    Tags

    KRAB-MeCP2

    Expression

    Mammalian

    Mutation

    Cas9 (D10A, H840A)

    Promoter

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tandem p40p35

    Plasmid

    #108665

    Purpose

    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker

    Depositor

    Nevil Singh

    Insert

    interleukin-12 (Il12a, Il12b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    IL-12 dimers have a Gly-Ser linker

    Promoter

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST53-LRRK2-G2019S

    Plasmid

    #25045

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Glycine 2019 mutated to Serine

    Promoter

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EF1A-CD19.8H.8TM.BB.3z

    Plasmid

    #200671

    Purpose

    Modular CD8hinge-CD8transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

    Depositor

    Michele Bernasconi

    Article

    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

    Insert

    FMC63-CD8HD-CD8TM-41BB-CD3z-P2A-EGFP (CD8A, TNFRSF9, CD247 Human)
    

    Use

    Lentiviral

    Tags

    MycTag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-short

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCINeoFlag ALIX

    Plasmid

    #89859

    Purpose

    Expresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SPLICSFAST-LY-MT-P2A

    Plasmid

    #214897

    Purpose

    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LY-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCLIP-tag (m) Vector

    Plasmid

    #101136

    Purpose

    Expression of protein of interest with CLIP-Tag in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Use

    Tags

    CLIP-tag (CLIP10)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pNCS mCherry

    Plasmid

    #91769

    Purpose

    Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    monomeric Cherry
    

    Use

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Promoter

    T7

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAV-Camk2-NES-Twitch-GR

    Plasmid

    #173044

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-GR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPB-CAG-dCas9-VPR-pgk-hph

    Plasmid

    #233066

    Purpose

    Piggybac CRISPRa construct

    Depositor

    Markus Landthaler

    Article

    Vučićević et al bioRxiv 2024.09.12.612198

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

    Plasmid

    #67981

    Purpose

    Cas9 activity reporter (control) with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CROPseq-Guide-Puro-mCherry2

    Plasmid

    #219679

    Purpose

    CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

    Depositor

    Christoph Bock

    Article

    Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Fuw-dCas9-Tet1CD-P2A-BFP

    Plasmid

    #108245

    Purpose

    Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A

    Promoter

    Ubc

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT3-EF1a-NICD1

    Plasmid

    #46047

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Hsp90 HA

    Plasmid

    #22487

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

    Insert

    Hsp90 HA (HSP90AB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge