159,371 results

1. pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

   Plasmid

   #210725

   Purpose

   NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   C2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCP30T-E2Crimson

   Plasmid

   #78478

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   E2-Crimson
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. NMD2

   Plasmid

   #216220

   Purpose

   Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns

   Depositor

   Rebecca Voorhees

   Article

   Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

   Insert

   GFP P2A RFP_b-globin intron x2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. C1-MPAct-mCherry

   Plasmid

   #155222

   Purpose

   Monitor changes in the density of membrane-proximal F-actin (MPA)

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-dSV40-CTLA-4-mGFP

   Plasmid

   #223594

   Purpose

   Lentiviral expression of human CTLA4 with mGFP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   mGFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV

   Plasmid

   #24828

   Depositor

   Lin He

   Article

   Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2923

   Plasmid

   #143674

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFIA (NFIA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ARHGEF11(DHPH)-CRY2-mCherry

   Plasmid

   #89481

   Purpose

   Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11

   Depositor

   Xavier Trepat Guixer

   Article

   Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

   Insert

   ARHGEF11(DHPH) - CRY2(1-498) - mCherry (CRY2, ARHGEF11 Mustard Weed, Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28 His6-mEGFP-D4

   Plasmid

   #226372

   Purpose

   Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4 (pfoA Clostridium perfringens)
   

   Use

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTTCC

    Plasmid

    #164264

    Purpose

    Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

    Depositor

    Gudmundur Hreggvidsson

    Article

    Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

    Inserts

    Cas9
    gRNA scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pcel and trc

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMTCbLCVA::CH42

    Plasmid

    #22786

    Depositor

    Niki Robertson

    Article

    Turnage et al Plant J. 2002 Apr . 30(1):107-14.

    Insert

    A DNA containing CHLI fragment
    

    Use

    RNAi; Arabidopsis vigs vector

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    July 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-X1-Neo-HA-UFL1

    Plasmid

    #139860

    Purpose

    Lentiviral expression of HA-tagged human UFL1

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    UFL1 (UFL1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs-puro GFP-p62

    Plasmid

    #38277

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAA3.stelN

    Plasmid

    #112011

    Purpose

    Expresses TelN enzyme

    Depositor

    Stephen Harrison

    Article

    Jenni et al Science. 2018 May 4;360(6388):552-558. d

    Insert

    TelN
    

    Use

    Tags

    strep and his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

    Plasmid

    #225708

    Purpose

    Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Fuw-dCas9-Tet1CD

    Plasmid

    #84475

    Purpose

    Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Tet1CD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. BNLS

    Plasmid

    #50836

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.2_mCherry_Grx1_roGFP2

    Plasmid

    #155045

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-VSP4-E228Q

    Plasmid

    #80351

    Purpose

    Expression of GFP-tagged, dominant negative VPS4 mutant

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    VPS4-E228Q (VPS4A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCB306-GFP-Rab12

    Plasmid

    #198471

    Purpose

    lentiviral expression of GFP-Rab12

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    GFP-Rab12 (RAB12 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pH-mono-MAD7

    Plasmid

    #170134

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    maize Ubiquitin-1, switchgrass Ubiquitin-1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAG-G-Flamp2-mut

    Plasmid

    #192783

    Purpose

    control probe of G-Flamp2, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Liu et al Front Pharmacol. 2022 May 12;13:902290.

    Insert

    G-Flamp2-mut
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV-Spike-VLP

    Plasmid

    #173922

    Purpose

    Expresses virus-like particles based upon the original SARS-Cov-2

    Depositor

    Andreas Stuermer

    Inserts

    SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 E protein (E SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 M Protein (M SARS-COV-2, Human, Synthetic)
    

    Use

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

    Available since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. mStrawberry-N1

    Plasmid

    #54644

    Purpose

    Localization: N1 Cloning Vector, Excitation: 574, Emission: 596

    Depositor

    Michael Davidson, Nathan Shaner, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Type

    Empty backbone

    Use

    Tags

    mStrawberry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PxylA

    Plasmid

    #55178

    Purpose

    Xylose-inducible promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    xylA promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    xylA

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRPN265NB-LaG16-MNase

    Plasmid

    #170978

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

    Insert

    Glutathione-S-Transferase/LaG16/MNase
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLEGFP-WT-STAT3

    Plasmid

    #71450

    Purpose

    Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

    Depositor

    George Stark

    Article

    Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

    Plasmid

    #192066

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Mouse Ctip2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-C1-A20

    Plasmid

    #22141

    Depositor

    Yihong Ye

    Article

    Li et al Biochim Biophys Acta. 2008 Jun . 1783(6)

    Insert

    A20 (TNFAIP3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FKBP-NTEVp(WT)

    Plasmid

    #137831

    Purpose

    Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-NTEVp(WT)
    

    Use

    Tags

    3x FLAG and NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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