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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,461 results

Showing: 10921 - 10960 of 159461 results

  1. PA-mCherry1-H2B-C-10

    Plasmid
    #57181
    Purpose
    Localization: Histones/Nucleus, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    57181-plasmid-map-sequence-id-256477

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  2. pB PUb-integrase

    Plasmid
    #183966
    Purpose
    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker
    Depositor
    Eric Marois
    Article
    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:
    Insert
    PhiC31 integrase
    Use
    Piggybac insect transgenesis destination vector
    Tags
    Expression
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183966-plasmid-map-sequence-id-361316

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  5. pcDNA3.3_CoV1_D28

    Plasmid
    #170447
    Purpose
    Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-1 spike D28 (S SARS-CoV-1)
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    last 28aa deletion in c-terminal tail
    Promoter
    CMV
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170447-plasmid-map-sequence-id-333040

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  17. mEos4b-CenpA-C-22

    Plasmid
    #62775
    Purpose
    Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Loren Looger, Michael Davidson
    Insert
    CenpA (CENPA Human)
    Use
    Tags
    mEos4b
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62775-plasmid-map-sequence-id-173170

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  18. pN3-Sp1FL

    Plasmid
    #24543
    Depositor
    Guntram Suske
    Insert
    Sp1FL (SP1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    24543-plasmid-map-sequence-id-209002

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  21. SELP-bio-His

    Plasmid
    #51639
    Purpose
    Expresses full-length P-selectin ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SELP (SELP Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51639-plasmid-map-sequence-id-78961

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  23. R777-E137 Hs.MTOR

    Plasmid
    #70421
    Purpose
    Gateway ORF clone of human MTOR [NM_004958.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MTOR (MTOR Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70421-plasmid-map-sequence-id-125870

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  28. pRAM28

    Plasmid
    #234314
    Purpose
    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications
    Depositor
    James Chappell
    Article
    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103
    Insert
    U1367 Targeting RAM, Barcode 2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    P. CymRC
    Available since
    March 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    234314-plasmid-map-sequence-id-466009

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  29. pBAD-bARGSer-AxeTxe

    Plasmid
    #192473
    Purpose
    Second-generation bacterial acoustic reporter gene derived from Serratia
    Depositor
    Mikhail Shapiro
    Article
    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Inserts
    bARGSer
    AxeTxe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    the gene Ser39006_001280 was deleted from the clu…
    Promoter
    Native promoter from Enterococcus faecium and pBAD
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    192473-plasmid-map-sequence-id-376957

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  31. PB-TO-hNIL BSD-mApple

    Plasmid
    #182313
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple
    Depositor
    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    bsd-T2A-mycNLS-mApple
    Use
    Tags
    T2A-mycNLS-mApple
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182313-plasmid-map-sequence-id-359511

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  37. mEos3.2-H1-10

    Plasmid
    #57457
    Purpose
    Localization: Nucleus/Histones, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    H1 (H1f0 Mouse)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    57457-plasmid-map-sequence-id-124124

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Showing: 10921 - 10960 of 159461 results