We narrowed to 30,618 results for: ica

1. pCI-SEP_NR2B

   Plasmid

   #23998

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   NR2B (Grin2b Rat)
   

   Use

   Tags

   NR2B signal sequence (aa1-31) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV.CAG.GLPLight1

   Plasmid

   #187467

   Purpose

   Expresses GLPLight1 in mammalian cells

   Depositor

   Tommaso Patriarchi

   Article

   Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

   Insert

   GLPLight1
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. His6_MBP_FXR1P

   Plasmid

   #198694

   Purpose

   Expression of human FXR1 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR1P (FXR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. His6_MBP_FMRP_P451S

   Plasmid

   #198693

   Purpose

   Expression of human FMRP protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FMR1 (FMR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed proline 451 to serine

   Promoter

   T7

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Vinculin-venus

   Plasmid

   #27300

   Depositor

   Martin Schwartz

   Article

   Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

   Insert

   Vinculin (VCL Chicken)
   

   Use

   Tags

   Venus A206K

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET14-GFP30_Encap

   Plasmid

   #86406

   Purpose

   Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 30aa CLP tag
   Encapsulin
   

   Use

   Tags

   6x Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-PspCas13b-gRNA-Actb1216

   Plasmid

   #155368

   Purpose

   PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   PspCas13b gRNA targeting Actb 1216
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET14-GFP15_Encap

   Plasmid

   #86407

   Purpose

   Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 15aa CLP tag
   Encapsulin
   

   Use

   Tags

   6x Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p6618 MSCV-IP N-HAonly WHSC2

   Plasmid

   #35014

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   WHSC2 (NELFA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Feb. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCytERM-mhYFP

    Plasmid

    #186530

    Purpose

    Visualization of the ER in cells using monomeric hyperfolder YFP (compatible with OSER assay)

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

    Plasmid

    #121944

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EcSTH-LbNOX

    Plasmid

    #211924

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.

    Depositor

    Binghui Li

    Article

    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

    Insert

    EcSTH-LbNOX
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTagRFP-C1-Fibrillarin

    Plasmid

    #70649

    Purpose

    Expresses Fibrillarin nucleolar marker with TurboRFP reporter.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    TagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCMT-flp

    Plasmid

    #67274

    Purpose

    Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

    Depositor

    Mark Liles

    Article

    Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

    Insert

    tetA, flp, repA101, oriR101
    

    Use

    Flp recombinase plasmid, temperature sensitive

    Tags

    His-SUMO (flp)

    Expression

    Mutation

    Promoter

    tetA promoter of plasmid pACYC184 origin

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-FLAG-LOV*-H2B

    Plasmid

    #202204

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to H2B in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    Histone H2B type 1-K
    

    Use

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCI-SEP_NR2A

    Plasmid

    #23997

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR2A (Grin2a Rat)
    

    Use

    Tags

    SEP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ArcLight-S249

    Plasmid

    #36855

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    simian CMV

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBR322-Pdp1_NTD-GFP-HiBiT

    Plasmid

    #198317

    Purpose

    Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-GFP-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS1NB c Rosella I

    Plasmid

    #71245

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pZmUBQ:LUC

    Plasmid

    #131860

    Purpose

    Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. talin1-head

    Plasmid

    #182996

    Purpose

    expression of talin1 head wt in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    talin1 head (Tln1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBMN DHFR(DD)-YFP

    Plasmid

    #29325

    Depositor

    Thomas Wandless

    Article

    Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

    Insert

    DHFR(DD)-YFP
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

    Promoter

    Available since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. OPN(-1206)-luc

    Plasmid

    #31106

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

    Insert

    osteopontin promoter (SPP1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    promoter region -1206 to +87

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZa pBAD csgA CBDcex pLac Ara csgGEF

    Plasmid

    #226378

    Purpose

    Plasmid carries csgA and csgG,E,F genes of curli operon

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    csgA and csgG,E,F genes of curli operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-EF1a-pMagHigh-FlpC

    Plasmid

    #180586

    Purpose

    PA-Flp

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Split FlpO
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV Flag WT-PTEN

    Plasmid

    #22231

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HLA_DRA_01_01

    Plasmid

    #165012

    Purpose

    Expression of HLA-DRA*01:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-DRA (HLA-DRA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHBV-Luc

    Plasmid

    #71414

    Purpose

    To see the activity of HBV transcription using Luciferase assay

    Depositor

    Wang-Shick Ryu

    Article

    Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

    Insert

    HBV core promoter, enhancer I, enhancer II and luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HBV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hSERT-N1-pEYFP

    Plasmid

    #70105

    Purpose

    mammalian expression of hSERT with C-terminal YFP fusion

    Depositor

    Harald Sitte

    Article

    Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

    Insert

    SERT (SLC6A4 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

    Plasmid

    #32476

    Purpose

    Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAM Y115F,L141F-5HT3 HC
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMCSG53-DsbC

    Plasmid

    #186623

    Purpose

    E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

    Depositor

    Alexei Savchenko

    Article

    Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

    Type

    Empty backbone

    Use

    Tags

    DsbC-6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pB-CAG-TEVp-IRES-mCherry

    Plasmid

    #174377

    Purpose

    For making TEVP cell line with hypBase

    Depositor

    Ian Wickersham

    Article

    Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

    Insert

    TEV Protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV.hSynap.iGABASnFR.F102G

    Plasmid

    #112160

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    Expression

    Mutation

    F102G

    Promoter

    hSynapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSMART-HCKan-yibDp-VHH-VCAM1

    Plasmid

    #202471

    Purpose

    Expresses the nanobody VCAM1 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VCAM1
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCIP-AMPKa1_WT

    Plasmid

    #79010

    Purpose

    For mammalian expression of WT AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGBW-m4046768

    Plasmid

    #145763

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pCLC3sGFP

    Plasmid

    #52423

    Purpose

    mammalian expression of rat CLC3 short transcript fused to EGFP

    Depositor

    Steven Weinman

    Article

    Li et al Am J Physiol Cell Physiol. 2002 Jun;282(

    Insert

    CLC3 (Clcn3 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEGFP-N1-Cdc45

    Plasmid

    #211426

    Purpose

    mammalian expression of human Cdc45 C-terminally fused to EGFP

    Depositor

    Helmut Pospiech

    Article

    Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

    Insert

    Cdc45 (CDC45 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MTK4_001

    Plasmid

    #123810

    Purpose

    Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Bgh pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge