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178,736 results

Showing: 8251 - 8280 of 178736 results

  1. Dead-Laconic/pcDNA3.1(-)

    Plasmid
    #118627
    Purpose
    To express in mammalian cells a lactate FRET sensor negative control.
    Depositor
    Luis Felipe Barros
    Publication
    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dead-Laconic
    Expression
    Mammalian
    Mutation
    Change Histidine 151 to Aspartic Acid of Escheric…
    Promoter
    CMV Promoter
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118627-plasmid-map-sequence-id-231761

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  8. AICSDP-42:AAVS1-mTagRFPT-CAAX

    Plasmid
    #107580
    Purpose
    Homology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cells
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    May 9, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    107580-plasmid-map-sequence-id-208159

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  9. pcDNA-NES-KIRIN1

    Plasmid
    #107416
    Purpose
    Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1 with nuclear exclusion sequence
    Depositor
    Robert Campbell
    Publication
    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIRIN1
    Tags
    Nuclear exclusion sequence (NES) (LALKLAGLDIGS)
    Expression
    Mammalian
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107416-plasmid-map-sequence-id-207270

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  10. pRPF215

    Plasmid
    #106377
    Purpose
    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon
    Depositor
    Robert Fagan, Neil Fairweather
    Publication
    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1
    Insert
    Ptet-himar1-Ter(slpA)-transposon
    Use
    E. coli - c. difficile shuttle vector
    Mutation
    Codon optimised for C. difficile
    Promoter
    Ptet
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106377-plasmid-map-sequence-id-205135

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  19. pJYS1Peftu

    Plasmid
    #85546
    Purpose
    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter
    Depositor
    Sheng Yang
    Publication
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Expression
    Bacterial
    Promoter
    etfu and unknown
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85546-plasmid-map-sequence-id-176968

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  20. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86612-plasmid-map-sequence-id-168849

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  24. mCyRFP1-C1

    Plasmid
    #84355
    Purpose
    Expresses mCyRFP1 fluorescent protein in an empty backbone
    Depositor
    Ryohei Yasuda
    Publication
    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCyRFP1
    Expression
    Mammalian
    Mutation
    A161K and N41A, reletive to CyRFP1
    Promoter
    CMV
    Available since
    Nov. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    84355-plasmid-map-sequence-id-160941

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  28. c3GIC9

    Plasmid
    #62191
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Publication
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. Pyogenes Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Promoter
    TRE3G
    Available since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62191-plasmid-map-sequence-id-156341

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  29. pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry

    Plasmid
    #74309
    Purpose
    Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian cells
    Depositor
    Ali Guler
    Publication
    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Rat, Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74309-plasmid-map-sequence-id-153122

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Showing: 8251 - 8280 of 178736 results