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pCVL-UCOE-SFFV-TLR-MCV1

Plasmid

#117111

Purpose

Lentiviral vector encoding Traffic light reporter 2.0. Alternative name: pCVL-UCOE-SFFV-TLR-MCV1.

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

UCOE SFFV Traffic Light Reporter 2.0

Use

Lentiviral; Reporter

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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ND4-DdCBE-right side TALE

Plasmid

#157843

Purpose

expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-NC

Plasmid

#105112

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Tags

cYFP-Myc and nYFP-HA

Expression

Plant

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MyrPalm-CKAR

Plasmid

#14862

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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TFORF1465

Plasmid

#142799

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP2K5

Plasmid

#20514

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K5 (MAP2K5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA8e-PcrA

Plasmid

#221228

Purpose

Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pVoPo-mCh

Plasmid

#187841

Purpose

Constitutive mCherry-expression vector for Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha

Plasmid

#21226

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIalpha (Camk2a Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0001.

Promoter

Available since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Use

Tags

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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TetOn-CLIMP63-mCherry-eGFP

Plasmid

#139871

Purpose

Dox-inducible lentiviral expression of human CLIMP63

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

CLIMP63 (CKAP4 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hPDGFRA-GFP

Plasmid

#22919

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human platelet derived growth factor receptor alpha polypeptide promoter driving GFP (PDGFRA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pIS0

Plasmid

#12178

Purpose

3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

Depositor

David Bartel

Article

Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pRL056 pDESTattB_exorh:EGFP

Plasmid

#213510

Purpose

attB-containing pDEST plasmid, for use in Multisite Gateway reaction

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis T5 Exonuclease

Plasmid

#206209

Purpose

Inducible expression of T5 exonuclease

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

T5 exonuclease

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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EGFR L858R

Plasmid

#11012

Purpose

Retroviral construct for expressing human EGFR with L858R mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR L858R (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

L858R (tumor-derived)

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Hsp90 HA

Plasmid

#22487

Depositor

William Sessa

Article

Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

Insert

Hsp90 HA (HSP90AB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-InteinC-CreC

Plasmid

#187615

Purpose

AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

InteinC-CreC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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p45sheLL

Plasmid

#37323

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

HPV45L1+L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJR103

Plasmid

#187242

Purpose

Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Jonathan Weissman, Marco Jost

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-PD-L1

Plasmid

#121492

Purpose

Expression of human PD-L1 WT in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1 (CD274 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-MYB

Plasmid

#51305

Purpose

Expression of MYB after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-myb avian myeloblastosis viral oncogene homolog (MYB Human)

Use

Lentiviral

Tags

n/a

Expression

Mutation

full length ORF

Promoter

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Use

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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ODD-Luciferase-pcDNA3

Plasmid

#18965

Depositor

William Kaelin

Article

Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

Insert

ODD-Firefly Luciferase (HIF1A Human)

Use

Tags

Expression

Mammalian

Mutation

HIF1a ODD sequence fused in frame to luciferase O…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-CUL1

Plasmid

#19896

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 1 (CUL1 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro2-H2B-iRFP

Plasmid

#197352

Purpose

Encoding nuclear-targeted iRFP713.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

iRFP with nucleus-targeting sequence

Use

Lentiviral

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAW63.YY1.FKBP.knock-in.BFP

Plasmid

#104371

Purpose

For knocking in FKBP F36V P2A BFP

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

FKBP12(F36V) -P2A-BFP

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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