162,160 results

1. pAAV.hSynapsin.SF-iGluSnFR.A184S

   Plasmid

   #106174

   Purpose

   Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1 and AAV9

   Insert

   SF-iGluSnFR.A184S
   

   Use

   AAV

   Tags

   Expression

   Mutation

   GltI: A184S

   Promoter

   hSynapsin

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-hNox4

   Plasmid

   #69352

   Purpose

   expresses human Nox4 in mammalian cells

   Depositor

   Karl-Heinz Krause

   Article

   Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

   Insert

   hNOX4 (NOX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME minprom KalTa4

   Plasmid

   #195964

   Purpose

   Gateway pME Gal4 variant

   Depositor

   Christian Mosimann, Alexa Burger

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mouse beta-globin minimal promoter KalTA4 (Gal4)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCaggs-mGold2s

   Plasmid

   #231763

   Purpose

   Expression of mGold2t in mammalian cells

   Depositor

   Francois St-Pierre

   Article

   Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

   Insert

   mGold2s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

   Promoter

   Actin promoter with CMV enhancer

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNC02

   Plasmid

   #233644

   Purpose

   For bacterial expression of sfGFP c-terminally tagged with VitelloTagB.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   sfGFP with c-terminal VitelloTagB
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

   Plasmid

   #228362

   Purpose

   mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Rfxcas13d grna expression …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

   Plasmid

   #170163

   Purpose

   Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2s-P2A-H2B-mRuby3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. LiOn-CMV∞Cre

   Plasmid

   #154018

   Purpose

   Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   Cre-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ubiquitin WT

   Plasmid

   #12647

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   Ubiquitin - wild type (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUFlip-floxed2A-mRFP-; HE1A:BFP -2

    Plasmid

    #180009

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVXT Sox4

    Plasmid

    #101121

    Purpose

    Sox4 coding region

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    SOX4 (Sox4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET His6 Sumo TEV LIC cloning vector (2S-T)

    Plasmid

    #29711

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, SUMO, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. ERK-KTR-mStrawberry reporter

    Plasmid

    #158686

    Purpose

    This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_hSyn1_AchLightG

    Plasmid

    #187464

    Purpose

    Expresses green acetylcholine indicator AchLightG in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightG
    

    Use

    AAV

    Tags

    Igκ leader

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23_Mmp11

    Plasmid

    #209520

    Purpose

    Expresses luciferase under control of Mmp11 promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

    Insert

    Mmp11 promoter (Mmp11 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

    Plasmid

    #163024

    Purpose

    Expression of CRISPRi with U6-gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 polyA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1a

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV5 mSynCAM3

    Plasmid

    #115498

    Purpose

    expression of mouse full-length SynCAM 3

    Depositor

    Thomas Biederer

    Article

    Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

    Insert

    Cadm3 (Cadm3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

    Plasmid

    #159575

    Purpose

    Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMyC

    Plasmid

    #42192

    Depositor

    Annabel Parret, Matthias Wilmanns

    Article

    Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    ColE1

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SIV_Gag_Pol

    Plasmid

    #236243

    Purpose

    3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SIV Gag and Pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mCherry-Peroxisomes-2

    Plasmid

    #54520

    Purpose

    Localization: Peroxisomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    Peroximal Targeting Signal 1 (PTS1) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SBE-NLS-mCherry PGK-rtTA3

    Plasmid

    #195332

    Purpose

    Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Inserts

    NLS-mCherry
    rtTA3
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and miniCMV

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid

    #218509

    Purpose

    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    mKate2
    

    Use

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Deletion of Plac_mrfp cassette, introduction of a…

    Promoter

    P23

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PCS2+Cas9-mSA

    Plasmid

    #103882

    Purpose

    Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    CAS9-MSA
    

    Use

    CRISPR

    Tags

    MSA (monomeric streptavidin

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1 SARS-CoV-2 N

    Plasmid

    #158079

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 nucleocapsid (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166013

    Purpose

    mammalian expression of MERS-CoV soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    MERS-CoV S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-ACE2-GFP

    Plasmid

    #154962

    Purpose

    pcDNA3.1-ACE2-GFP

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    ACE2-GFP (ACE2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cherry-Fibrillarin-C1

    Plasmid

    #134539

    Purpose

    Expresses Cherry-Fibrillarin fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    Cherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSCV-LMP1-IRES-mCherry

    Plasmid

    #220351

    Purpose

    Expresses EBV latent gene LMP1 in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP1 (LMP-1 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge