179,521 results
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Plasmid#124213PurposeInsect cell expression vector for His6, Spy tagged hsDDB1∆BDepositorInsertDNA damage-binding protein 1 (DDB1 Human)
TagsHis6-SpyExpressionInsectMutationinternal deletion of BPB domain to aid crystalliz…Available sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZ149-pcDNA3-BhCas12b_v4
Plasmid#122446PurposeExpresses BhCas12b_v4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV-NLS-BhCas12b_v4-NLS-3xHA
ExpressionMammalianAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-CDK4
Plasmid#116724PurposeLentiviral expression of CDK4DepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-6p1-GST-ArcFL
Plasmid#119877PurposeBacterial Expression construct for protein production and purificationDepositorAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK346 (Hygro-P2A-mAID-mCherry)
Plasmid#121180PurposeN-terminal taggingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHygro-P2A-mAID-mCherry
UseCRISPRAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMaCTag-P05
Plasmid#120016PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteGFP
UsePcr templateTagseGFPPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseExpressionBacterialAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-GFP11-pDEST-N
Plasmid#118366PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible N-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FlagExpressionMammalianAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV Flag ATRwt
Plasmid#41909DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATR ATR serine/threonine kinase (ATR Human)
TagsFLAGExpressionMammalianPromoterSRaAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX311-Cas9
Plasmid#118018PurposeConstitutive expression of Cas9DepositorInsertCas9
UseLentiviralExpressionMammalianPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRE145
Plasmid#118497Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
ExpressionBacterialAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR1316
Plasmid#111126PurposeExpresses FANCA.DepositorAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDe-CAS9
Plasmid#61433PurposeBinary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequenceDepositorInsertCas9
UseCRISPRExpressionPlantPromoterPcUBI4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-NES-NIR-GECO1
Plasmid#113683PurposeExpress NIR-GECO1 preferentially in neuronsDepositorInsertNES-NIR-GECO1
UseAAVPromoterhSynAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIGAhRC
Plasmid#112510Purposehuman Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoterDepositorUseSynthetic BiologyExpressionYeastPromoterGal1,10Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
Tags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-FLAG- gate-pGK-HYG
Plasmid#107397PurposeGateway destination vectorDepositorTypeEmpty backboneUseGateway Cloning and LentiviralTagsFLAGExpressionMammalianAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV.rTH.PI.Cre.SV40
Plasmid#107788PurposeAAV vector expressing Cre from rTH promoterDepositorHas serviceAAV9 and AAV RetrogradeInsertCre
UseAAVExpressionMammalianPromoterrTHAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX551-miniCMV-SpCas9
Plasmid#107031PurposeAAV plasmid expressing Cas9 under control of miniCMV promoterDepositorInsertSpCas9
UseAAV and CRISPRExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_LKB1
Plasmid#108111PurposeLentiviral expression plasmid of human LKB1 cDNA with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
WT Smarca4-sfGFP
Plasmid#107056PurposeExpression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationwild-typePromoterCAGAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pN3-Nipbl
Plasmid#107716PurposeExpression plasmid for 3xFLAG-tagged NIPBLDepositorAvailable sinceMarch 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST
Plasmid#105448PurposeCation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vectorDepositorHas serviceAAV9InsertChrimsonR-mRuby2-ST
UseAAVTagsmRuby2 and soma targeting motifMutationK176R in ChrimsonPromoterhSynAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-hSynI-rtTAV16
Plasmid#102367PurposeAAV vector to drive TET-ON activator expression from human synapsin I promoterDepositorInsertrtTAV16
UseAAVExpressionMammalianMutationrtTA variant reported in Gene Therapy (2006) 13, …Promoterhuman synapsin I promoterAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC NLS HA stdMCP Halo
Plasmid#104098PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.DepositorInsertstdMCP-Halo
UseLentiviralTagsC-terminal Halo-tag, N-terminal NLS, and Two syno…ExpressionMammalianAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS_VP35_EBOV
Plasmid#103050PurposeEncodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
ExpressionMammalianPromoterCAGAvailable sinceDec. 20, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pHel2
Plasmid#102960PurposeE.coli/H.Pylori shuttle vectorDepositorTypeEmpty backboneUsePlasmid shuttle vector system for genetic complem…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCLXSN-c-JUN
Plasmid#102758PurposeExpression of c-JunDepositorAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-flag-hKDM4A-polyA
Plasmid#101051Purposein vitro transcription (T7 promoter)DepositorAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIRECT_25H
Plasmid#91145PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-DIO-EGFP
Plasmid#87168PurposeExpression of eGFP in the presence of CreDepositorInsertEGFP
UseAAVTagsNoneAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCM189-ParB1::mCherry
Plasmid#87253PurposeExpresses ParB1-mCherry in yeast. Also known as pOR1-mchDepositorInsertParB1
TagsmCherryExpressionYeastPromoterpBAD/tetOAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCM184-ParB2::eGFP
Plasmid#87252PurposeExpresses ParB2-EGFP in yeast. Also known as pOR2-GFPDepositorInsertParB2
TagsEGFPExpressionYeastPromoterpBAD/tetOAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPRi_Mxi1_yl
Plasmid#91248PurposeCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-Mxi1
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-mTurquoise2
Plasmid#99113PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2DepositorInsertmTurquoise2
UseAAVExpressionMammalianPromoterTREAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIRECT_21C
Plasmid#91130PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p3E_BAG3wt-eGFP
Plasmid#98180PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBAG3wt-eGFP (BAG3 Human)
UseGateway vectorTagsGFPAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN244 - CTCF-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#92140PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using BLASTICIDIN selection. Auxin-inducible degron system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAID[71-114]-eGFP
UseMouse TargetingExpressionMammalianAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs
Plasmid#86828PurposeExpression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequenceDepositorAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-FLEX-eNpHR-EYFP
Plasmid#89876PurposeTo generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoterDepositorInserteNpHR=EYFP
UseAAVTagsEYFPExpressionMammalianPromoterTetOAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only