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JDW 947 (pME-mmTcf4_DN_P2A_mCherry)
Plasmid#232122PurposeA middle entry gateway clone containing myc-tagged murine dominant negative Tcf4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.DepositorInsertTcf4ΔN (Tcf4 Mouse)
UseGateway cloningTagsmycExpressionMutationlacks aa 1-56PromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor
Plasmid#229789PurposeBxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2DepositorInsertmNgn2; ZF10-NS3-p65 (Neurog2 Mouse)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF10 and CAGAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-SYNDAPIN2
Plasmid#221311PurposeExpression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transductionDepositorInsertSYNDAPIN2 (pLxIS) (Pacsin2 Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD-IFP2.0C
Plasmid#215750PurposeExpresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD_W42A-IFP2.0C
Plasmid#215751PurposeExpresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD_W42A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-MMACHC [N230/21R]
Plasmid#114555PurposeMammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.DepositorInsertanti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
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H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Oct3/4-IRES-Neo
Plasmid#13461DepositorInsertOct3/4 (Pou5f1 Mouse)
UseTagsExpressionMammalianMutationP31SPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-mR7BD
Plasmid#79149Purposebacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)DepositorInsertR7BD (Rilp Mouse)
UseTagsGSTExpressionBacterialMutationamino acids 220-299PromotertacAvailable sinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-Irf7
Plasmid#178445Purposedoxycycline-inducible expression of Mouse Irf7 in mammalian cellsDepositorInsertIrf7 (Irf7 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pmCherry-C1-mTrim21
Plasmid#105516PurposeExpression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-Gsdmd
Plasmid#80950PurposeExpresses Gsdmd in mammalian cellsDepositorInsertGsdmd (Gsdmd Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTight-9-124-BclxL
Plasmid#60857Purpose2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoterDepositorInsertmiR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterpTightAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W
Plasmid#67986PurposeCas9 activity reporter with mCherry and BFPDepositorInsertsU6gRNA cassette, PGKmCherryABFP cassette, WPRE
Guide RNA targeting modified BFP
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro-KrasG12D2B
Plasmid#139786PurposeLentiviral tet-inducible expression of KrasG12D (mouse)DepositorInsertKrasG12D (Kras Mouse)
UseLentiviralTagsExpressionMammalianMutationG12DPromoterAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only
