We narrowed to 2,903 results for: BFP

1. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

   Plasmid

   #219690

   Purpose

   Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

   Plasmid

   #217970

   Purpose

   Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

   Depositor

   Robert Piper

   Article

   Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

   Insert

   2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(G116A)

   Plasmid

   #212108

   Purpose

   Expression of lipidation deficient GABARAP split tandem protein with mTagBFP2

   Depositor

   Dieter Willbold

   Article

   Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

   Insert

   GABARAP(G116A)-mTagBFP2-GABARAP(G116A) (GABARAP Entacmaea quadricolor, Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   G116A (lipidation deficiency) of both GABARAPs

   Promoter

   CMV

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

   Plasmid

   #204729

   Purpose

   Cre-inducible CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

   Plasmid

   #204727

   Purpose

   Frt-inducible CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

   Plasmid

   #204724

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCA20-HSPB1 5'HA-mTagBFP-3'-UTR 12XMS2V5-3'HA (HDR donor)

   Plasmid

   #199451

   Purpose

   CRISPR donor plasmid to insert [mTagBFP-stop-12XMS2] into the C-terminus of human HSPB1 gene; 12XMS2 locates in the 3' UTR region

   Depositor

   Baohui Chen

   Article

   Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

   Insert

   HDR donor template
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)

   Plasmid

   #191147

   Purpose

   Expression of the sequence matching the 54 extra bases in EK0362 in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   Bdfn.t+54
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-Rncp-iGluSnFR1

   Plasmid

   #187093

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-Rncp-iGluSnFR1
   

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHIE061 ZF43x6-C mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138742

    Purpose

    Integration Vector, assembled with pHIE045 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHIE065 ZF43x12-S mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138746

    Purpose

    Integration Vector, assembled with pHIE049 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Spaced
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

    Plasmid

    #159844

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138740

    Purpose

    Integration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x1
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHIE060 ZF43x3-C mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138741

    Purpose

    Integration Vector, assembled with pHIE044 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x3-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138744

    Purpose

    Integration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x3-Spaced
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138745

    Purpose

    Integration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Spaced
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHIE062 ZF43x12-C mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138743

    Purpose

    Integration Vector, assembled with pHIE046 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Compact
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]

    Plasmid

    #133330

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All FYW to L in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]

    Plasmid

    #133331

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All VLIM to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]

    Plasmid

    #133332

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #118808

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All F to S in CTD of full-length TDP43

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHBS1187 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 N-Q]

    Plasmid

    #107847

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All asparagine to glutamine in splicing reporter

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHBS1185 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]

    Plasmid

    #107843

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All phenylalanine to serine in splicing reporter

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #210525

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-rev(CMV-TagBFP2)-EFS-mCherry-206-MRE

    Plasmid

    #235156

    Purpose

    Expresses the mCherry-based sensor for miR-206. TagBFP2 is expressed as a transduction reporter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    mCherry with a miR-206 MRE from Tpm4
    

    Use

    AAV

    Promoter

    EFS

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid

    #217971

    Purpose

    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.

    Depositor

    Robert Piper

    Article

    Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

    Insert

    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    

    Expression

    Mammalian

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

    Plasmid

    #60910

    Purpose

    dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, and NLS

    Expression

    Mammalian

    Promoter

    dSV40 Promoter

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

    Plasmid

    #235086

    Purpose

    GFP splicing reporter with CORO1B intron

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    custom bidirectional

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

    Plasmid

    #191159

    Purpose

    Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP:EGFP.s2:mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre

    Plasmid

    #84561

    Purpose

    pSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loops

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    FLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
    

    Use

    Lentiviral

    Tags

    Flag, Suntag, oxBFP, AID (auxin-inducible degron)

    Promoter

    UbC

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

    Plasmid

    #235087

    Purpose

    GFP splicing reporter with FRG1 intron

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    custom bidirectional

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

    Plasmid

    #223213

    Purpose

    Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    TGFb1 active - membrane (TGFB1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)

    Plasmid

    #128608

    Purpose

    Expresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    36xMashTag
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid

    #194885

    Purpose

    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 and -2 targeting the human MECP2 locus.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Inserts

    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1and2
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    Human codon optimized catalytically inactive LbCp…

    Promoter

    TRE and U6

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid

    #64218

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E1B

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204723

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP

    Plasmid

    #223211

    Purpose

    Lentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    CD25 (IL2RA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)

    Plasmid

    #128607

    Purpose

    Expresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    36xMashTag
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199363

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6

    Plasmid

    #64220

    Purpose

    Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E4orf6

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTS98 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-MCS

    Plasmid

    #199351

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

    Plasmid

    #64219

    Purpose

    Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA targeting ROSA26-1
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-BFP-P2A-E1B

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHR- GAL4-UAS-BAMH1-IRES-mCherry-PGK-BFP

    Plasmid

    #223215

    Purpose

    Lentiviral vector - mCherry reporter with a BAMH1 site for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    mCherry BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

    Plasmid

    #78251

    Purpose

    Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Inserts

    -3mnx1 (mnx1 Zebrafish)
    -loxP-mTagBFP2-stop-
    mCherry-NS-MCS
    

    Use

    Zebrafish motor neuron inducible expression

    Promoter

    -3mnx1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

    Plasmid

    #184952

    Purpose

    Donor for both PiggyBac and Cp36 Recombinase

    Depositor

    Patrick Hsu, Ami Bhatt, Lacra Bintu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    PuroR, P2A, mTagBFP2
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid

    #176531

    Purpose

    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

    Depositor

    Jared Toettcher

    Article

    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

    Inserts

    KGV transcription factor
    TagBFP
    

    Use

    Piggybac transposase integration vector

    Tags

    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

    Expression

    Mammalian

    Available Since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSLPB2B-ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187950

    Purpose

    ecDHFR degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ecDHFR-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187948

    Purpose

    ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    ER50-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187951

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)

    Plasmid

    #191162

    Purpose

    Expression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP:s70587:mCherry
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge