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We narrowed to 7,219 results for: cas9 plasmid

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Showing: 1351 - 1400 of 7219 results

  7. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107300
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107300-plasmid-map-sequence-id-204631

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  8. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107299
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP
    Use
    CRISPR
    Tags
    3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107299-plasmid-map-sequence-id-204615

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  10. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid
    #121841
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121841-plasmid-map-sequence-id-237395

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  16. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid
    #234737
    Purpose
    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.
    Depositor
    Kirill Martemyanov
    Article
    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:
    Insert
    Nrl (Nrl Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    234737-plasmid-map-sequence-id-466566

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  17. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid
    #131757
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#2 Sp/xCas9 gRNA
    Use
    Gateway Cloning and Synthetic Biology
    Promoter
    hU6
    Available Since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    131757-plasmid-map-sequence-id-314450

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  18. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid
    #131756
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#1 Sp/xCas9 gRNA
    Use
    Gateway Cloning and Synthetic Biology
    Promoter
    hU6
    Available Since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    131756-plasmid-map-sequence-id-314449

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  19. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid
    #131755
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#1 SaCas9 gRNA
    Use
    Gateway Cloning and Synthetic Biology
    Promoter
    hU6
    Available Since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    131755-plasmid-map-sequence-id-314448

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  20. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

    Plasmid
    #131758
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#2 SaCas9 gRNA
    Use
    Gateway Cloning and Synthetic Biology
    Promoter
    hU6
    Available Since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131758-plasmid-map-sequence-id-314395

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  21. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  25. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187960
    Purpose
    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187960-plasmid-map-sequence-id-373524

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  26. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

    Plasmid
    #223167
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE
    Depositor
    Boris Kantor
    Article
    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223167-plasmid-map-sequence-id-442870

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  27. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187963
    Purpose
    mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Inserts
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker and T2A
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187963-plasmid-map-sequence-id-373709

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  28. KK701: pMAGIC (L3-L2) 3x HA eptitope tag + polyA; hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121842
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven xCas9(3.7) gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA fusion to dCas9 w/ gRNA expression
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121842-plasmid-map-sequence-id-237396

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  29. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

    Plasmid
    #223166
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE
    Depositor
    Boris Kantor
    Article
    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223166-plasmid-map-sequence-id-442868

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  30. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

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  31. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  34. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid
    #187959
    Purpose
    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Inserts
    Blasticidin resistance
    FKBP12_F36V
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187959-plasmid-map-sequence-id-373708

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  35. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available Since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  38. CRISPR plasmid

    Plasmid
    #236553
    Purpose
    Encodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Cas9
    Expression
    Mammalian
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236553-plasmid-map-sequence-id-469881

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  42. Srs2-C-terminus_pET28a

    Plasmid
    #247774
    Purpose
    The primary use of this plasmid is as a target for CRISPR-Cas9. It can also be used to express the His-tagged C-terminus of Srs2 with Lys-to-Arg mutations.
    Depositor
    Yun-Seok Choi
    Insert
    Srs2-C-terminus (NEWENTRY Budding Yeast)
    Mutation
    The C-terminus of the gene has Lys-to-Arg mutatio…
    Promoter
    T7
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    247774-plasmid-map-sequence-id-508625

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  44. pITI2

    Plasmid
    #166095
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Rebecca Burgess
    Insert
    Cas9-D10A
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    166095-plasmid-map-sequence-id-335585

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  45. pPH3

    Plasmid
    #166098
    Purpose
    Gal inducible expression of D10A-Cas9 nickase
    Depositor
    Rebecca Burgess
    Insert
    Cas9-D10A
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A
    Promoter
    Gal-inducible
    Available Since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166098-plasmid-map-sequence-id-330351

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  46. TC9pAB

    Plasmid
    #71310
    Purpose
    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9
    Use
    In vitro transcription
    Tags
    NLS
    Promoter
    T7
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71310-plasmid-map-sequence-id-129410

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  47. pHEE-R-kan5A9si

    Plasmid
    #222836
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, kanamycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222836-plasmid-map-sequence-id-446487

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  48. pHEE-R-kan9si

    Plasmid
    #222833
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, kanamycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222833-plasmid-map-sequence-id-446484

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  50. pHEE-R-hyg9si

    Plasmid
    #222832
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222832-plasmid-map-sequence-id-446482

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Showing: 1351 - 1400 of 7219 results