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We narrowed to 63,650 results for: cloning

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Showing: 541 - 560 of 63650 results

  2. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129025-plasmid-map-sequence-id-454365

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  3. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Publication
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9_D10A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Available since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41816-plasmid-map-sequence-id-116715

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  4. BPK3082

    Plasmid
    #78742
    Purpose
    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCpf1 crRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78742-plasmid-map-sequence-id-239497

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  9. pMOD_C3106

    Plasmid
    #91101
    Purpose
    Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VPR backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Promoter
    FMV 34S
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91101-plasmid-map-sequence-id-182063

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  10. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129027
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129027-plasmid-map-sequence-id-256129

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  15. pAG/MNase

    Plasmid
    #123461
    Purpose
    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.
    Depositor
    Steven Henikoff
    Publication
    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1
    Insert
    Protein A-Protein G-Micrococcal Nuclease
    Tags
    6xHIS and HA
    Expression
    Bacterial
    Promoter
    Lac
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123461-plasmid-map-sequence-id-417805

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  16. PB-TAC-ERP2

    Plasmid
    #80478
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Publication
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80478-plasmid-map-sequence-id-404034

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  17. pKSB-AeU6-3gRNA

    Plasmid
    #183914
    Purpose
    gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter
    Depositor
    Eric Marois
    Publication
    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold for protospacer cloning into BbsI sites
    Promoter
    Aedes aegypti U6 promoter (AAEL017763)
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183914-plasmid-map-sequence-id-361376

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  18. pMOD_C3003

    Plasmid
    #91095
    Purpose
    Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP (+ BaeI site for GT donor cloning)
    Use
    Unspecified
    Promoter
    CmYLCV
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91095-plasmid-map-sequence-id-181832

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  19. CRISPRoff-v2.1

    Plasmid
    #167981
    Purpose
    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter
    Depositor
    Luke Gilbert
    Publication
    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167981-plasmid-map-sequence-id-330241

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Showing: 541 - 560 of 63650 results