161,273 results

1. pET21a-CysRS-His

   Plasmid

   #124107

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Cysteinyl-tRNA ligase (cysS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSR33.4r

   Plasmid

   #63198

   Purpose

   Expresses Cph8, Ho1, and PcyA constitutively

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

   Inserts

   Cph8
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mem-mCherry-pTRIP

   Plasmid

   #163521

   Purpose

   Used to construct lentivirus to express membrane-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   membrane targeted mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ePPE-spG

   Plasmid

   #183096

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2022 Mar 24. doi: 10.103

   Insert

   Nls-nspGCas9-NC-nls-MLV-Nls
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpGCas9

   Promoter

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pALPS vpu

   Plasmid

   #101330

   Purpose

   Encodes codon optimized NL4-3 vpu

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 vpu
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pROSA26-1

   Plasmid

   #21714

   Depositor

   Philippe Soriano

   Article

   Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mEGFP-CaMKIIa

   Plasmid

   #127389

   Purpose

   Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 HA Arf6 DN T27N

   Plasmid

   #10831

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 T27N (ARF6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T27N. Defective in GTP binding. Dominant negati…

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304 3xFlag-eRF1 G183/184A_IRES-GFP

   Plasmid

   #198394

   Purpose

   eRF1 (AAQ mutant) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   eRF1 (ETF1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   mutated GGQ motif (G183/184A)

   Promoter

   CMV

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-endo-EKAR4

    Plasmid

    #205513

    Purpose

    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-EKAR4
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a-HisRS-His

    Plasmid

    #124111

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Histidyl-tRNA ligase (hisS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4xM67 pTATA TK-Luc

    Plasmid

    #8688

    Depositor

    Jim Darnell

    Article

    Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

    Insert

    4 repeated M67 sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_hU6-peg(ATP1A1-T804N)_mU6-BsmBI_Puro

    Plasmid

    #234070

    Purpose

    For lentiviral expression of user-specified pegRNA in tandem with epegRNA (ATP1A1-T804N) for ouabain co-selection

    Depositor

    Greg Findlay

    Article

    Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-(SUMO)3

    Plasmid

    #127093

    Purpose

    Bacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    EGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSH231-EF1-RFP-HYGRO

    Plasmid

    #115145

    Purpose

    Safe harbor site 231 knock-in vector with RFP-HygromycinR expression cassette

    Depositor

    Raymond Monnat, Michael Phelps

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLZ12Km2-P23R:TA:Xen5

    Plasmid

    #88902

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + LuxABCDE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pMT899

    Plasmid

    #228288

    Purpose

    Constitutive mutant rPhlTA expression with strong promoter

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    rphlTA mutant
    

    Use

    Synthetic Biology

    Tags

    Three copies of VP16 (VP48)-Nuclear localization …

    Expression

    Yeast

    Mutation

    P5S, S6P, K86T, F109L, Q117R, E143K

    Promoter

    KpGAPDH promoter

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. hMLKL NB(1-182)-2xFv-Venus

    Plasmid

    #106077

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    human MLKL NB(1-182)-2xFv-Venus (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    amino acids 1-182

    Promoter

    Dox-inducible

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCMV_DENV3_PrME

    Plasmid

    #207265

    Purpose

    Mammalian expression of Dengue virus 3 PrME protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DENV3_PrME (POLY Dengue virus 3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAW-Nb127D01-GFP

    Plasmid

    #171571

    Purpose

    Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-GFP (CXCR2 Human)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    fly actin5C promoter

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid

    #223508

    Purpose

    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

    Inserts

    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y306A; Y384F

    Promoter

    CVM, SP6 and U6

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV-lyso-pHoenix

    Plasmid

    #70112

    Purpose

    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat, Mouse)
    

    Use

    Tags

    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAcCas12nHS_ABE_D3_empty

    Plasmid

    #203809

    Purpose

    Plasmid containing a CMV-driven NLS-dAcCas12n(D240)-ABE-Design3-NLS cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion).

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    ABE_D3
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pPAGFP-VSVG

    Plasmid

    #11915

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (L64F/T65S/V163A/T203H). The PAGFP also contains …

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-mGFP-Actin

    Plasmid

    #21948

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

    Insert

    betaActin (ACTB Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pABY-T1

    Plasmid

    #201764

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG cyto-RCaMP1h

    Plasmid

    #105014

    Purpose

    cytosolic calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pc3.1-CAGGS-ASAP3-mCyRFP3

    Plasmid

    #193323

    Purpose

    A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

    Depositor

    Michael Lin

    Article

    Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

    Insert

    ASAP3-R3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

29. p3xFLAG-CMV10-hFIP200

    Plasmid

    #24300

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    FIP200 (RB1CC1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. fluoPEER-GFP>BFP

    Plasmid

    #185477

    Purpose

    Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

    Depositor

    Sabine Fuchs

    Article

    Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

    Insert

    GFP-Y66-editing-site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHIV-NAT-FLAG-CIP2A FL (sg2R)

    Plasmid

    #222624

    Purpose

    Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

    Insert

    CIP2A (CIP2A Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1-alpha promoter

    Available since

    July 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pDV09_pCMV-ADAR1-p110

    Plasmid

    #200404

    Purpose

    Expresses ADAR1 (p110 isoform) constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    ADAR-p110
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBAC-ECFP-15xQUAS_TATA-mcd8-GFP-SV40

    Plasmid

    #104878

    Purpose

    piggyBac vector of 15xQUAS-mCD8:GFP; used for mosquito tranformation

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    mCD8:GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Puro.Cre empty vector

    Plasmid

    #17408

    Purpose

    Lentiviral expression of Cre from the mouse PGK promoter

    Depositor

    Tyler Jacks

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

    Insert

    Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse PGK

    Available since

    March 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJW1311

    Plasmid

    #61254

    Purpose

    Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting Y61A9L9.1
    

    Use

    CRISPR; Cloning template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. RUNX1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176974

    Purpose

    Mammalian expression vector encoding RUNX1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. H2B-GFP-Aurora B WT

    Plasmid

    #184042

    Purpose

    Expresses H2B-GFP fused to Aurora B WT

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    EGFP and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. FH-TET3-pEF

    Plasmid

    #49446

    Purpose

    This plasmid expresses full-length human TET3.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    Tet methylcytosine dioxygenase 3 (TET3 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. scaffold-linker-U6

    Plasmid

    #89639

    Purpose

    construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFUGW NLS-FlpO

    Plasmid

    #183448

    Purpose

    Lentiviral FlpO vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    NLS-FlpO
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge