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171,784 results

Showing: 8431 - 8460 of 171784 results

  3. YopH (residues 164-468)

    Plasmid
    #79749
    Purpose
    This plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    YopH (residues 164-468)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79749-plasmid-map-sequence-id-443904

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  7. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #128589
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV8
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    Syn
    Available Since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128589-plasmid-map-sequence-id-257684

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  8. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Expression
    Mammalian
    Available Since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

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  9. pE-SUMO CRYGD

    Plasmid
    #80755
    Purpose
    Expression of human gamma D-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYGD (CRYGD Human)
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80755-plasmid-map-sequence-id-153570

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  17. pGreenFire1_E2F1RE

    Plasmid
    #112248
    Purpose
    expression of GFP and Luciferase under the control of E2F1 responsive element
    Depositor
    Simone Di Giovanni
    Insert
    E2F1RE (responsive element)
    Use
    Lentiviral
    Tags
    copGFP and luciferase
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    112248-plasmid-map-sequence-id-213659

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  19. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  22. pRPA32-EGFP

    Plasmid
    #211383
    Purpose
    mammalian expression of human RPA32 C-terminally fused to EGFP
    Depositor
    Helmut Pospiech
    Insert
    RPA32 (RPA2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    211383-plasmid-map-sequence-id-420377

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  24. mEmerald-Desmoglein1-C-18

    Plasmid
    #54061
    Purpose
    Localization: Desmosomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Desmoglein 1 (DSG1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54061-plasmid-map-sequence-id-444157

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  30. pD649-HAsp-LILRB4-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156692
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    LILRB4 (LILRB4 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156692-plasmid-map-sequence-id-409999

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Showing: 8431 - 8460 of 171784 results