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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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164,223 results

Showing: 8431 - 8460 of 164223 results

  1. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid
    #200402
    Purpose
    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection
    Depositor
    James Collins
    Article
    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:
    Insert
    Closed-loop DART VADAR sensor entry cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200402-plasmid-map-sequence-id-396788

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  5. pCdc45-mCherry

    Plasmid
    #211380
    Purpose
    mammalian expression of human Cdc45 C-terminally fused to mCherry
    Depositor
    Helmut Pospiech
    Insert
    Cdc45 (CDC45 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211380-plasmid-map-sequence-id-420495

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  8. pDest-566

    Plasmid
    #11517
    Depositor
    Dominic Esposito
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    6xHis and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    May 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11517-plasmid-map-sequence-id-299808

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  14. TFORF0033

    Plasmid
    #141485
    Purpose
    Lentiviral vector for overexpressing the SP1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SP1 (SP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141485-plasmid-map-sequence-id-276591

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  15. pMDlg/pRRE-int-MS2M

    Plasmid
    #166032
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    Gag-Pol-MS2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166032-plasmid-map-sequence-id-338747

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  20. Matrix-roGFP

    Plasmid
    #49437
    Purpose
    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells
    Depositor
    Paul Schumacker
    Article
    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi
    Insert
    Matrix-roGFP2
    Use
    Adenoviral
    Tags
    Cytochrome c oxidase subunit IV mitochondrial tar…
    Expression
    Mammalian
    Mutation
    C48S, Q80R, S147C, and Q204C in Clontech's G…
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49437-plasmid-map-sequence-id-257693

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  23. Flag-APEX2-C Cloning Vector

    Plasmid
    #128146
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Flag-APEX2
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128146-plasmid-map-sequence-id-248610

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  25. pHTNW

    Plasmid
    #136403
    Purpose
    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.
    Depositor
    Sebastian Maurer
    Article
    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:
    Type
    Empty backbone
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    136403-plasmid-map-sequence-id-272878

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  26. pYS17

    Plasmid
    #202083
    Purpose
    Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in
    Depositor
    Daniel Dickinson
    Article
    Shi et al Development. 2023 May 15;150(10):dev2013
    Insert
    NT PuroR-T2A-mNG Minicircle Parent
    Use
    CRISPR
    Tags
    mNeonGreen
    Expression
    Mutation
    Promoter
    None
    Available Since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202083-plasmid-map-sequence-id-409950

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  27. AAV-GFP/Cre

    Plasmid
    #49056
    Purpose
    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.
    Depositor
    Fred Gage
    Article
    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99
    Insert
    Cre
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    EGFP, Myc, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    49056-plasmid-map-sequence-id-182646

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  30. pCINEO-3xFlag-hRIPK3

    Plasmid
    #112495
    Purpose
    Mammalian expression of 3xFlag-hRIPK3
    Depositor
    Junying Yuan
    Insert
    RIPK3 (RIPK3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112495-plasmid-map-sequence-id-214535

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Showing: 8431 - 8460 of 164223 results