We narrowed to 17,665 results for: She

1. pCMV-Tag2-Sox9 T236A

   Plasmid

   #111455

   Purpose

   Transient expression of Flag-tagged human Sox9 with T236A mutation

   Depositor

   Sharon Pine

   Article

   Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

   Insert

   Sox9 (SOX9 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T236A mutation

   Promoter

   CMV

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB3-miRFP670nano

   Plasmid

   #127433

   Purpose

   EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   EB3-miRFP670nano (MAPRE3 Synthetic, Human)
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBMN Parkin

   Plasmid

   #89299

   Purpose

   For expression of untagged Parkin

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   Parkin (PRKN Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCardinal-Tubulin-C-18

   Plasmid

   #56176

   Purpose

   Localization: Microtubules, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pcDNA3-FLAG-MTOR-F1888L

   Plasmid

   #69010

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-F1888L (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Phenylalanine 1888 to Leucine

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. TOP2A sgRNA1

   Plasmid

   #138190

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOP2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TOP2A sgRNA2

   Plasmid

   #138191

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TOP2A

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TOP2A (TOP2A Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV_RBMX-eA3A-UdgX-nCas9

   Plasmid

   #163546

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MEIS1A-MIY

   Plasmid

   #21013

   Depositor

   Corey Largman

   Article

   Fischbach et al Blood. 2005 Feb 15. 105(4):1456-66.

   Insert

   MEIS1A (Meis1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES and YFP

   Expression

   Mammalian

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

10. HiBiT-FZD3

    Plasmid

    #195841

    Purpose

    Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD3 (FZD3 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    I292V and V495I

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCardinal-PDHA1-N-10

    Plasmid

    #56172

    Purpose

    Localization: Mitochondrial Matrix, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    PDHA1 (PDHA1 Human)
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pcDNA5 FRT TO GFP hBub1 K821R

    Plasmid

    #59813

    Purpose

    Allows the integration of GFP hBub1 K821R in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    K821R

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218867

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_-CMV-Pcdh15_mini_V4-CD2

    Plasmid

    #199184

    Purpose

    An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

    Depositor

    David Corey

    Article

    Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

    Insert

    mmPcdh15_mini_V4-CD2
    

    Use

    AAV

    Mutation

    EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UbC-mEmerald Rab7a

    Plasmid

    #115238

    Purpose

    To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab7a fused to mEmerald (RAB7A Human)
    

    Use

    AAV

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    7 Amino Acids (21 Nucleotides) between mEmerald a…

    Promoter

    Human UbC

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCKO_LacZ_sgRNA

    Plasmid

    #74179

    Purpose

    lentiviral vector expressing sgRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    LacZ sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330 LC-FKBP-Cas9

    Plasmid

    #162724

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE2-TetOminiCMV-Sox2

    Plasmid

    #136612

    Purpose

    Dox-inducible lentiviral vector expressing mouse Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV_eA3A T31A-dCas9

    Plasmid

    #163537

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    dCas9 (D10A, H840A)eA3A T31A

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHAGE2-TetOminiCMV-cMyc

    Plasmid

    #136614

    Purpose

    Dox-inducible lentiviral vector expressing mouse cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    cMyc (Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Arrb1-Q394L

    Plasmid

    #35402

    Depositor

    Robert Lefkowitz

    Article

    Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

    Insert

    beta-arrestin-1 (Arrb1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q394L

    Promoter

    CMV

    Available Since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

23. p5E-TetO8x-CMVmin (JDW 1087)

    Plasmid

    #224475

    Purpose

    Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TetO(8x)-CMV promoter
    

    Use

    Gateway cloning

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5 FRT TO GFP hBub1 WT

    Plasmid

    #59812

    Purpose

    Allows the integration of GFP hBub1 in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Perera et al J Cell Sci. 2010 Mar 1;123(Pt 5):653-9.

    Insert

    Bub1 (BUB1 Human)
    

    Tags

    EmGFP

    Expression

    Mammalian

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

    Plasmid

    #203803

    Purpose

    Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Inserts

    RAB10 (RAB10 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q68L

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Stargazin-dCherry-FRB

    Plasmid

    #172444

    Purpose

    Expression of plasma membrane protein Stargazin tagged with dark (non-fluorescent) mCherry-FRB

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cacng2 (Cacng2 Mouse)
    

    Tags

    dCherry-HA-FRB

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSCV-human CSFI-R-L301S- Y969F-IRES-GFP

    Plasmid

    #133745

    Purpose

    retroviral expression of CSF1-R mutant

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF1-R (CSF1R Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    L301S- Y969F

    Promoter

    MSCV

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. single polypeptide FPX biosensor for both caspase-3 and -8

    Plasmid

    #60886

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-3 and 8
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

    Plasmid

    #192160

    Purpose

    Dual AAV vector AAV-dCas9

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Insert

    Dead Cas9
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX_317-C7orf26v1

    Plasmid

    #178537

    Purpose

    Subcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_317 backbone

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Insert

    C7orf26v1 (INTS15 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Human HOXA9-MSCV-short

    Plasmid

    #20978

    Depositor

    Corey Largman

    Article

    Vijapurkar et al Mol Cell Biol. 2004 May . 24(9):3827-37.

    Insert

    HOXA9 (HOXA9 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Missing amino acid #1. Missing 3'UTR. Coding…

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA3-V5-PTPN14-PPxY

    Plasmid

    #61006

    Purpose

    Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 PPxY mutation (PTPN14 Human)
    

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    PPxY mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

33. tet H-ras val12

    Plasmid

    #8900

    Depositor

    Lynda Chin

    Article

    Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

    Insert

    ras val12 (HRAS Human)
    

    Tags

    Tet-op promoter

    Expression

    Mammalian

    Mutation

    G12V

    Available Since

    Jan. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pLenti-Blast PhLP1-myc-6xHis

    Plasmid

    #218178

    Purpose

    Used to make a lenti virus for stable expression of phosducin-like protein 1 in mammalian cells

    Depositor

    Barry Willardson

    Article

    Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

    Insert

    Phosducin like protein 1 (PDCL Human)
    

    Use

    Lentiviral

    Tags

    6x His and Myc

    Expression

    Mammalian

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEN25-Ub Hs

    Plasmid

    #83232

    Purpose

    pHis-par2-Ub Hs

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Ubiqutin (UBC Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-Tag2-Sox9 T240A

    Plasmid

    #111457

    Purpose

    Transient expression of Flag-tagged human Sox9 with T240A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T240A

    Promoter

    CMV

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEB31 (hANGPTL8-V5-His lenti)

    Plasmid

    #109116

    Purpose

    V5 and His-tagged human ANGPTL8 in a lentiviral vector

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL8 (ANGPTL8 Human)
    

    Use

    Lentiviral

    Tags

    6x His and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid

    #218865

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8s
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBMN HA-GBRP

    Plasmid

    #89296

    Purpose

    For expression of the mammalian Atg8 protein GABARAP tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

40. pCDNA3-V5-PTPN14-del-N

    Plasmid

    #61004

    Purpose

    Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 delete N (PTPN14 Human)
    

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 17-310

    Promoter

    CMV

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only