-
Plasmid#181830PurposeGenetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.DepositorInsertLyn-PlcR
UseTagsECFP, EYFP, and N-terminal targeting sequence fro…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
mKate1.31-pBAD
Plasmid#54827PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635DepositorTypeEmpty backboneUseTags6xHis and mKate1.31ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDAS12125_PEAR-GFP
Plasmid#177178PurposePEAR-GFP target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H TC editing reporter
Plasmid#198882PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralTagsExpressionMammalianMutationY93HPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.cytGAP
Plasmid#78734PurposeExpression of Ca2+ sensor GAP in the cytosolDepositorInsertcytGAP
UseTagsluciferaseExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-HpaBC
Plasmid#71260PurposeExpresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6DepositorInsertsHpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRU55
Plasmid#167684PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.690
Plasmid#108533PurposePcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.DepositorInsertPcaUAM + P3B5B-YFP
UseTagsExpressionBacterialMutationPcaU: K14N A165T N201HPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pmRuby2-CrowdingLinker-Clover
Plasmid#171058PurposeExpresses tandem mClover2-mRuby2 FRET pair with a crowdedness-sensitve linker domain to sense cytosolic crowdedness.DepositorInsertCrowdedness-sensitive linker domain
UseTagsmClover2 and mRuby2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJM.1642
Plasmid#108535PurposeCinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.DepositorInsertCinRAM + PCin-YFP
UseTagsExpressionBacterialMutationCinR: S72C V80I G114D G115D R137H K220R A224T *24…PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG018 (AVAST type 2)
Plasmid#157896PurposeExpresses the AVAST type 2 defense systemDepositorInsertAVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-iGECInano
Plasmid#186188PurposeAAV vector packaging small FRET-based near-infrared genetically-encoded calcium indicatorDepositorInsertiGECInano
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE6
Plasmid#79476PurposesgRNA shuttle vector; module 1EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS423-GPD-wrmScarlet1-10-CYC1 terminator
Plasmid#158584PurposeExpresses wrmScarlet1-10 in S. cerevisiaeDepositorInsertwrmScarlet1-10
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 iTEV
Plasmid#64277PurposeExpresses infrared fluorescent tobacco etch virus protease sensor (iTEV)DepositorInsertInfrared fluorescent tobacco etch virus protease sensor (iTEV)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Mito-7
Plasmid#58115PurposeLocalization: Mitochondria, Excitation: 554, Emission: 581DepositorInsertMito (COX8A Human)
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.iGABASnFR.F102G
Plasmid#112160PurposeGABA SensorDepositorInsertiGABASnFR.F102G
UseAAVTagsExpressionMutationF102GPromoterhSynapsinAvailable sinceJuly 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsA
Plasmid#107558PurposePlasmid for titrating synthesis of FtsA with doxycyclineDepositorInsertftsA
UseSynthetic BiologyTagsExpressionMutationPromoterpLtetOAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-E2-Crimson
Plasmid#111252PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-CrimsonDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC3EGFP
Plasmid#193790PurposeExpresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterPR promoterAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ManII-SBP-mCherry
Plasmid#65256Purposesynchronize trafficking of ManII from the ERDepositorInsertIi-Streptavidin and truncated Mannosidase II
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
UseTagsExpressionBacterialMutationNahR: I25V P72H V118A R248CPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cx35-Halo-C-end
Plasmid#71512PurposeMammalian expression plasmid encoding perch Cx35 with a C-terminal HaloTag.DepositorInsertConnexin 35
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-gRNA
Plasmid#65626PurposeEmpty vector for the expression U6 driven gRNADepositorInsertU6-gRNA
UseTagsExpressionMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV_PACLight1
Plasmid#197864PurposeMammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)DepositorInsertPACLight1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPLtetO-FtsZ
Plasmid#89656PurposePlasmid for titrating synthesis of FtsZ with doxycyclineDepositorInsertGTP-binding tubulin-like cell division protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only