We narrowed to 11,832 results for: NSI;
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Plasmid#103124PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ03LF7(Cas9 coding gene from Nitrobacter hamburgensis (strain X14 / DSM 10229))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-D1VXP4
Plasmid#103093PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertD1VXP4(Cas9 coding gene from Prevotella timonensis CRIS 5C-B1)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E7H313
Plasmid#103104PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE7H313(Cas9 coding gene from Sutterella wadsworthensis 3_1_45B)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-TetOn-mCherry-OAZ1_FS-YFP
Plasmid#232356PurposeMammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)DepositorInsertOAZ1 derived polyamine sensing module (OAZ1 Human)
UseLentiviralTagseYFP and mCherryExpressionMammalianPromoterTRE3GAvailable SinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUltraI-Tet3.0[TAA]
Plasmid#164580PurposeMachinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codonsDepositorInsertsMb Tet3.0RS (R2-3)
Mm tRNA[TAA]
ExpressionBacterialMutationL170G, N311G, C313APromoterProK and TacIAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP E2F4
Plasmid#236439Purposetransient overexpression of E2F4 in mammalian cellsDepositorAvailable SinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 2xFLAG-2xSTREP E2F7
Plasmid#236442Purposetransient overexpression of E2F7 in mammalian cellsDepositorAvailable SinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-tdMCP-Erk2 (pErk reporter)
Plasmid#226167PurposeUsed to transiently express the pErk reporter in LABEL-seq MAPK activity assays.DepositorAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ARR3tk-eGFP/SV40-mCherry
Plasmid#132360PurposeTo measure transcriptional activity of Androgen ReceptorDepositorInsertAR responsive elements (AR Human)
UseLentiviralAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN017-SpCas9-hiNLS-t-M4
Plasmid#234455PurposeExpresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO (pEHA1339)
Plasmid#209088PurposeBackbone plasmid for Tet Responsive transgene expression, FRT integrationDepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFPc1 IP3R3 WT
Plasmid#236453Purposetransient overexpression of IP3R3 in mammalian cellsDepositorInsertIP3R3 (Itpr3 Rat)
ExpressionMammalianAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP T184M
Plasmid#114181PurposeORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIDTSmart-CMV-PLRS1
Plasmid#217365PurposeExpression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"DepositorInsertleucyl-tRNA synthetase polyspecific mutant
ExpressionMammalianMutationM40I, T252A, Y499I, Y527A, H529GPromoterCMVAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMarsL274G
Plasmid#63177PurposeThis plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.DepositorAvailable SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 FLAG FBXW7alpha
Plasmid#236470Purposetransient overexpression of FBXW7alpha in mammalian cellsDepositorInsertFBXW7alpha (FBXW7 Human)
ExpressionMammalianAvailable SinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA
Plasmid#115161PurposeThis plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.DepositorInsertsPTRE - tet activator responsive promoter
flex sequence
hM3dq-mCherry (inverted)
flex sequence
UseAAVTagshM3Dq-mCherryExpressionMammalianAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 FLAG FBXO31
Plasmid#236429Purposetransient overexpression of FBXO31 in mammalian cellsDepositorAvailable SinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLPC-Progerin
Plasmid#69061Purposeencodes the lamin A mutant with a 50 amino acids deletion responsible for the Hutchinson-Gilford Progeria SyndromeDepositorAvailable SinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-polyQ74-NLS
Plasmid#231892PurposeForms nuclear HTT partial exon 1 Q74-NLS aggregatesDepositorInsertHTT partial exon 1 Q74-NLS (HTT Human)
TagsEGFP and SV40 NLSExpressionMammalianMutationCAG repeat expansionAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pP{ELAV-GeneSwitch}
Plasmid#83957PurposeExpresses conditional RU486-dependent GAL4 protein under control of the elav promotorDepositorInsertExpressionInsectPromoterelavAvailable SinceDec. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLS13-IL-10
Plasmid#209128PurposeTransient mammalian expression of IL-10DepositorInsertIL-10 (IL10 Human)
ExpressionMammalianAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP Omomer
Plasmid#113169PurposeRetroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.DepositorInsertOmomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
UseRetroviralExpressionMammalianMutationE57T, E64I, R70Q, R71NAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
ExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-EcTrp-TGA
Plasmid#228780PurposeaaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codonDepositorInsertsEcTrpRS
EcWtR
UseBaculoviralExpressionMammalianMutationTGA suppressorAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-monSTIM1
Plasmid#161795PurposeMammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)DepositorAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)
Plasmid#236421Purposetransient overexpression of KEAP1 in mammalian cellsDepositorInsertKEAP1 (Keap1 Mouse)
ExpressionMammalianAvailable SinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr230-EGFP-3XNLS
Plasmid#58466PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoterDepositorInsertsTagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-EcTyr-TAG
Plasmid#228782PurposeEcTyr aaRS/tRNA system for mammalian ncAA incorporationDepositorInsertsEcTyrRS
EcYtR
BstR
UseBaculoviralExpressionMammalianMutationTAG suppressorAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFPc3 K-RAS 4B
Plasmid#236452Purposetransient overexpression of K-RAS 4B in mammalian cellsDepositorInsertK-RAS 4B (KRAS Human)
ExpressionMammalianAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
CoV2-Spike-EF1a-D614G-N501Y
Plasmid#177939PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.DepositorAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-BacMam-4x-PyOtR
Plasmid#217364PurposeExpresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmidDepositorInsert4 copies of pyrrolysyl-tRNA mutant PyOtR
UseBaculoviralExpressionInsect and MammalianPromoterU6Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule2 - Mj Acd A9
Plasmid#197652PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
ExpressionBacterialPromoterGlnS (constitutive)Available SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
sc-WDR24-SEH1L
Plasmid#237418PurposeTransient expression of human WDR24-SEH1L "single chain" in the GATOR2 complexDepositorAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP
Plasmid#176027Purposeconstruct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometryDepositorInsertsrtTA3
Luc2
mtagBFP
UseLentiviralAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKS070 - pCAGGS-3XFLAG-(human)CTCF-eGFP
Plasmid#156448PurposeFor transient expression of human CTCF tagged with eGFPDepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Is3xP3-mCherry
Plasmid#220091PurposeExpresses in ISE6 (Ixodes scapularis) cells, heat inducible. Promoter consists of 3 Pax 6 sites from 3xP3 with a minimal IsHSP70 promoter. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)DepositorInsert3 Pax6 binding sites from 3xP3 with minimal HSP70 promoter from Ixodes scapularis
PromoterIs3xP3Available SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
DRH313: FCK-CheRiff-eGFP
Plasmid#51693PurposeChannelrhodopsin with improved sensitivity and minimal red light photocurrentDepositorInsertCheRiff-eGFP
UseLentiviralTagseGFPExpressionMammalianPromoterCaMKIIaAvailable SinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only