We narrowed to 22,836 results for: Sis

1. pET-Impact-T4gp61(I342G)

   Plasmid

   #228208

   Purpose

   Expression in BL21(DE3) of T4 primase I342G

   Depositor

   Stephen Benkovic

   Article

   Valentine et al Biochemistry. 2001 Dec 18;40(50):15074-8

   Insert

   61 (61 Escherichia phage T4)
   

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Mutated isoleucine 342 to glycine (I342G), improv…

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. rTetR-GFP-p65

   Plasmid

   #232633

   Purpose

   p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

   Depositor

   Karsten Rippe

   Insert

   rTetR-GFP-p65 (RELA Human, Synthetic)
   

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-GFP-p65

   Plasmid

   #232630

   Purpose

   p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

   Depositor

   Karsten Rippe

   Insert

   dCas9-GFP-p65 (RELA Human, Synthetic)
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mCherry)

   Plasmid

   #233542

   Purpose

   A CMV promoter driving expression of a murine dominant negative myc tagged TCF4.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF4 (TCF4 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Mutation

   lacks aa 1-56

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

   Plasmid

   #232128

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA2 (GATA2 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

   Plasmid

   #232124

   Purpose

   A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF7 (TCF7 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

   Plasmid

   #232123

   Purpose

   A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

   Plasmid

   #232129

   Purpose

   A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   MAFB (MAFB Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

   Plasmid

   #232131

   Purpose

   A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   FOXF2 (FOXF2 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

    Plasmid

    #232130

    Purpose

    A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 1036 (pME_V5_TCF7L2_P2A_H2A_mCherry)

    Plasmid

    #232126

    Purpose

    A middle entry gateway clone containing V5 tagged human TCF7L2 / TCF4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7L2 / TCF4 (TCF7L2 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNDGG002

    Plasmid

    #231308

    Purpose

    Vector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNDGG001

    Plasmid

    #231307

    Purpose

    Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACYCDuet-AtTIR1-Myc-MBP-AtIAA6-HA

    Plasmid

    #222708

    Purpose

    Expression AtTIR1-Myc and MBP-AtIAA6-HA in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Inserts

    AtTIR1 (TIR1 Mustard Weed)
    AtIAA6 (IAA6 Mustard Weed)
    

    Tags

    HA, MBP, and myc

    Expression

    Bacterial

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+ HA-BEX1

    Plasmid

    #231002

    Purpose

    Transient overexpression of human BEX1 in mammalian cells

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

    Insert

    BEX1 (BEX1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFNC-8

    Plasmid

    #227670

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. AmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    AmR
    

    Expression

    Bacterial

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYCDuet-1:ChrA-C63S-C66S

    Plasmid

    #225082

    Purpose

    Expresses precursor peptide ChrA-C63S/C66S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C63S/C66S
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C63S/C66S

    Promoter

    T7

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Tensin-2 Y483E

    Plasmid

    #201794

    Purpose

    Expresses Tensin-2 protein with Y483E mutation in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    mutation Y483E

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Tensin-2 Y483F

    Plasmid

    #201795

    Purpose

    Expresses Tensin-2 protein with Y483F mutation in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    mutation Y483F

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Tensin-2 Y483A

    Plasmid

    #201796

    Purpose

    Expresses Tensin-2 protein with Y483A mutation in MAC-Tag-C backbone

    Depositor

    Vesa Hytönen

    Article

    Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

    Insert

    Tensin-2 (Tns2 Mouse)
    

    Tags

    BirA, HA, strepIII

    Expression

    Bacterial and Mammalian

    Mutation

    mutation Y483A

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pACYCDuet-1:ChrA-C66S

    Plasmid

    #225081

    Purpose

    Expresses precursor peptide ChrA-C66S in E.coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

    Insert

    ChrA-C66S
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C66S

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEMT-mMyh13CDS

    Plasmid

    #192853

    Purpose

    Plasmid to produce ISH probe for mMyh13 gene with CDS sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 13 (Myh13 Mouse)
    

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEMT-mMyh13UTR

    Plasmid

    #192854

    Purpose

    Plasmid to produce ISH probe for mMyh13 gene with UTR sequence

    Depositor

    Masafumi Inui

    Article

    Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

    Insert

    myosin heavy chain 13 (Myh13 Mouse)
    

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMRX-IPU-GFP-Rab5

    Plasmid

    #228139

    Purpose

    Expresses GFP-Rab5 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Rab5 (Rab5a Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. OSCA1.2-K435L-L438K-peGFP-N1

    Plasmid

    #228587

    Purpose

    Expresses OSCA1.2-K435L-L438K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    K435L, L438K

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pR-mOrange2

    Plasmid

    #228608

    Purpose

    Mammalian expression of mOrange2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mOrange2
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pR-mBeRFP

    Plasmid

    #228601

    Purpose

    Mammalian expression of mBeRFP, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mBeRFP
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pR-LSSmOrange

    Plasmid

    #228600

    Purpose

    Mammalian expression of LSSmOrange, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    LSSmOrange
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pR-mTFP1

    Plasmid

    #228602

    Purpose

    Mammalian expression of mTFP1, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTFP1
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pM-EBFP2

    Plasmid

    #228614

    Purpose

    Mammalian expression of EBFP2 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    EBFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pM-mT-Sapphire

    Plasmid

    #228616

    Purpose

    Mammalian expression of mT-Sapphire and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mT-Sapphire
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pM-mAmetrine

    Plasmid

    #228617

    Purpose

    Mammalian expression of mAmetrine and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mAmetrine
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pM-mCerulean3

    Plasmid

    #228618

    Purpose

    Mammalian expression of mCerulean3 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mCerulean3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pM-EGFP

    Plasmid

    #228622

    Purpose

    Mammalian expression of EGFP and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pM-mVenus

    Plasmid

    #228625

    Purpose

    Mammalian expression of mVenus and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mVenus
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pM-miRFP670

    Plasmid

    #228631

    Purpose

    Mammalian expression of miRFP670 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    miRFP670
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMRX-IPU-SNAP-Omp25

    Plasmid

    #228137

    Purpose

    Expresses SNAP-Omp25 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    transmembrane region (C-terminus)

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHelper-CRISPRi-sgRNA

    Plasmid

    #221135

    Purpose

    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with dCas9 handle
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119(SpeI)

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDEST17-NUDT5

    Plasmid

    #219955

    Purpose

    For protein purification from Ecoli

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    NUDT5 (NUDT5 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDEST17-LAP3

    Plasmid

    #217661

    Purpose

    For protein purification from Ecoli

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    LAP3 (LAP3 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge