159,530 results

1. pmIL-6 FL

   Plasmid

   #61286

   Purpose

   drives luciferase from Wt mouse IL-6 promoter (full length)

   Depositor

   Gail Bishop

   Article

   Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

   Insert

   IL-6 promoter (Il6 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   IL-6 full length promoter

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-GalT

   Plasmid

   #11929

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Promoter

   Available since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE-puro SHP2 WT

   Plasmid

   #8329

   Depositor

   Ben Neel

   Article

   Zhang et al Mol Cell Biol 2002 Jun;22(12):4062-72.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-Ets2

   Plasmid

   #28128

   Depositor

   Pumin Zhang

   Article

   Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

   Insert

   Ets2 (ETS2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRFP-Ub

   Plasmid

   #11935

   Depositor

   Nico Dantuma

   Article

   Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

   Insert

   Ubiquitin (RBX1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CalfluxCTN

   Plasmid

   #190068

   Purpose

   Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

   Depositor

   Carl Johnson

   Article

   Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

   Insert

   CalfluxCTN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-MAP1LC3C

   Plasmid

   #137758

   Purpose

   Expression of LC3C

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   MAP1LC3C (MAP1LC3C Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CFP-Parkin

   Plasmid

   #47560

   Purpose

   CFP tagged Parkin for colocalization study

   Depositor

   Richard Youle

   Article

   Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUdO1a

    Plasmid

    #210226

    Purpose

    high-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUCM-CLYBL-hNIL

    Plasmid

    #105841

    Purpose

    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-myc-Atg7

    Plasmid

    #24921

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    Atg7 (ATG7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAF0034 Ef1a-Kp03-2a-EGFP

    Plasmid

    #193461

    Purpose

    Expression of Kp03 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AiP15745: pAAV-AiE0452h-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5745)

    Plasmid

    #230880

    Purpose

    AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-cT3G-cERP2-GATA4

    Plasmid

    #192896

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Codon-optimized

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-Cre-GFP

    Plasmid

    #68544

    Purpose

    AAV expression of Cre-GFP fusion

    Depositor

    Eric Nestler

    Article

    Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTTCC

    Plasmid

    #164264

    Purpose

    Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

    Depositor

    Gudmundur Hreggvidsson

    Article

    Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

    Inserts

    Cas9
    gRNA scaffold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pcel and trc

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGIG

    Plasmid

    #11159

    Purpose

    Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Has Service

    Cloning Grade DNA

    Insert

    IRES-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRE-Tn7-141

    Plasmid

    #118557

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET28 His6-mEGFP-D4

    Plasmid

    #226372

    Purpose

    Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4 (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET28a_Pro-IL-33

    Plasmid

    #214342

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL33 (IL33 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. pQE-60 Grx1-roGFP2-His

    Plasmid

    #64799

    Purpose

    Bacterial expression of His-tagged Grx1-roGFP2

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    His and roGFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid

    #105539

    Purpose

    AAV expression of EGFP from hSyn promoter

    Depositor

    James M. Wilson

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3.1/hChR2(H134R)-mCherry

    Plasmid

    #20938

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    June 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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26. mScarlet-I-LaminB

    Plasmid

    #98831

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. FLAG-JAK1

    Plasmid

    #174572

    Purpose

    Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    JAK1 (JAK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAS161

    Plasmid

    #194484

    Purpose

    synER Cassette

    Depositor

    Ahmad Khalil

    Article

    Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

    Insert

    synER Cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-GGamma2-GFP2

    Plasmid

    #166774

    Purpose

    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma2-GFP2 (GNG2 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains an N-terminal GFP2 and GSAG linker

    Promoter

    CMV

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pTriEx-NTOM20-LOV2

    Plasmid

    #81009

    Purpose

    Expression of LOV2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Use

    Tags

    NTOM20

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV, p10

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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