164,223 results

1. HA Depdc5 pRK5

   Plasmid

   #46327

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Differs from Depdc5 isoform 1 by 10 amino acids …

   Promoter

   Available Since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pLKO-FOXM1-shRNA-81

   Plasmid

   #89495

   Purpose

   Lentiviral vector with constitutive expression of shRNA targeting human FOXM1.

   Depositor

   Adam Karpf

   Insert

   FOXM1 (FOXM1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-EGFP

   Plasmid

   #89875

   Purpose

   EGFP reporter vector of iTango system for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   tetO-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

   Plasmid

   #209077

   Purpose

   Ngn2 mediated cortical neuron induction, dox induction is included

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Inserts

   NGN2 (Neurog2 Mouse)
   SNAPtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE4G

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-OSKM

   Plasmid

   #20328

   Purpose

   Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

   Depositor

   Rudolf Jaenisch

   Article

   Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

   Insert

   Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIN BRCA2 WT

   Plasmid

   #16245

   Depositor

   Mien-Chie Hung

   Article

   Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

   Insert

   BRCA2 (BRCA2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-51b(+)_NAD-Snifit

   Plasmid

   #113658

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB501

   Plasmid

   #74843

   Purpose

   Gateway cloning compatible binary vector for simple cloning of gene.

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. hACE2

   Plasmid

   #1786

   Purpose

   Mammalian expression of human ACE2

   Depositor

   Hyeryun Choe

   Article

   Li et al Nature. 2003 Nov 27. 426(6965):450-4.

   Has Service

   Cloning Grade DNA

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 14, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBad-EBFP2

    Plasmid

    #14891

    Purpose

    Bacterial expression of EBFP2 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Has Service

    Cloning Grade DNA

    Insert

    EBFP2
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Promoter

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. bact2-mKate2-P2A-TurboID-dVHH[GFP]

    Plasmid

    #163857

    Purpose

    Zebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBH

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    TurboID-VHH[EGFP]
    

    Use

    Zebrafish expression

    Tags

    mKate2-P2A-TurboID

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-5'-ENGRAM

    Plasmid

    #179157

    Purpose

    piggyBac plasmid for 5' ENGRAM recorder

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minP

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZL Neo Myr Flag DLG5

    Plasmid

    #20477

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    DLG5 (DLG5 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

14. CoV2-M-IRES-E

    Plasmid

    #177938

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. GAL4BD-VP16/pDON207

    Plasmid

    #201230

    Purpose

    GAL4-VP16 transcription factor

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    GAL4BD-VP16
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHiUGE smFP-V5 donor ORF0

    Plasmid

    #200380

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHiUGE smFP-V5 donor ORF1

    Plasmid

    #200381

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSL-MS2-12X

    Plasmid

    #27119

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2 binding sites (12)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT-TO-DEST

    Plasmid

    #106348

    Purpose

    Destination vector for gateway cloning. Developed for Flp-In T-REx system.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-UltraCas12a-6xNLS

    Plasmid

    #209023

    Purpose

    Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    UltraCas12a-6xNLS-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    M537R/F870L

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. GPR124-Tango

    Plasmid

    #66312

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR124 (ADGRA2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Str-KDEL_SBP-EGFP-Ecadherin

    Plasmid

    #65286

    Purpose

    synchronize trafficking of Ecadherin from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

    Plasmid

    #52521

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Use

    AAV and Cre/Lox

    Tags

    2xHA on ChR2

    Expression

    Mutation

    Promoter

    CBA

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSEP-LAMP1-RFP

    Plasmid

    #167970

    Purpose

    Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

    Depositor

    Juan Bonifacino

    Article

    Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    SEP and mRFP

    Expression

    Mammalian

    Mutation

    D50E

    Promoter

    CMV

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Dual-CRISPR-Cas9-U6-H1 (pBP48)

    Plasmid

    #218932

    Purpose

    The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    2A-Puro and 3XHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSWAP_Entry_H1_alpha

    Plasmid

    #184865

    Purpose

    pSwap plasmid part with H1_alpha homology arms

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pCAG-HA-NLGN3 R451C

    Plasmid

    #59319

    Purpose

    Expression plasmid of HA-tagged human Neuroligin 3 harboring an R451C mutation

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN3 R451C (NLGN3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Arginine 451 to Cysteine (R451C mutation)

    Promoter

    CAG

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Use

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR- flag-Lag9-iCAM-1

    Plasmid

    #205209

    Purpose

    This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG9

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and Lag9 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge