We narrowed to 7,452 results for: Lif;
-
Plasmid#119896PurposeMouse NRTN In situ hybridization probeDepositorInsertneurturin probe (Nrtn Mouse)
UseIn situ hybridization probeTagsExpressionMutationPromoterAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph0
Plasmid#231897PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 0.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph1
Plasmid#231898PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 1.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph2
Plasmid#231899PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
NBS1-Halo HRD
Plasmid#207084PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous NBS1 locus.DepositorInsertHaloTag followed by a LoxP-PuroR cassette flanked by human NBS1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
Rev7-Halo HRD
Plasmid#207079PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous REV7 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human REV7 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-MDC1 HRD
Plasmid#207086PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous MDC1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human MDC1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATM HRD
Plasmid#207085PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous ATM locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human ATM locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
ATM-Halo HRD
Plasmid#207082PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-53BP1 HRD
Plasmid#207087PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-SHLD3 HRD
Plasmid#207077PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD3 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human SHLD3 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-SHLD1 HRD
Plasmid#207076PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD1 locus.DepositorInsertHaloTag flanked by human SHLD1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
hSyn-tractin
Plasmid#187649PurposeExpresses F-tractin (from ITPKA)-mNeongreen under control of human synapsin promoter.DepositorInsertF-tractin
UseAAVTagsmNeonGreenExpressionMammalianMutationPromoterSynapsinAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-HsTtyh1-TwinStrep-His
Plasmid#211332PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)DepositorInsertTweety homology protein 1
UseLentiviralTagsTwinStrep, 10xHisExpressionMammalianMutationW417RPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rif1-Halo HRD
Plasmid#207081PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF169-Halo HRD
Plasmid#207083PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.DepositorInsertHaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-SHLD2 HRD
Plasmid#207078PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nef-AO-66-71-TVA950
Plasmid#217977PurposeAAV Transfer plasmid to express TVA950 in a Cre-dependent mannerDepositorInsertTVA950
UseAAVTagsExpressionMutationPromoternEFAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_R261A
Plasmid#202586PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Arginine 261 to AlaninePromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAEA
Plasmid#202588PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, changed O…PromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
MsACR1-pmCerulean3-N1
Plasmid#204958PurposeExpression of channelrhodopsin MsACR1 fused to mCerulean3 in mammalian cellsDepositorInsertMsACR1
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMV (+enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)
Plasmid#193050PurposeEncodes guide RNA expression targeting PX740 landing padDepositorInsertGuide RNA
UseCRISPRTagsExpressionWormMutationPromoterU6Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L 2xHis-Rab29
Plasmid#186021Purpose2xHis-Rab29DepositorInsert2xHis-Rab29 (RAB29 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-RedO-shNC
Plasmid#206356PurposeAAV plasmid expressing non-targeting control shRNA in photoreceptorsDepositorInsertNon-targeting control shRNA
UseAAVTagsExpressionMutationPromoterhuman red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Txnip(C247S)
Plasmid#206353PurposeAAV plasmid expressing mutant TXNIPDepositorInsertTxnip (Txnip Mouse)
UseAAVTagsExpressionMutationC247SPromoterCMVAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSHLP 16-218
Plasmid#180609PurposeBacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83DepositorInsertAMSHLP residues 16-218
UseTagsHIS-SUMOExpressionBacterialMutationResidues 16-218PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP
Plasmid#185679PurposeLow-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.DepositorInsertERmembrane-2xHaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 V192S
Plasmid#175769PurposeLentiviral vector expressing N-terminally tagged GFP-TAX1BP1 V192SDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationV192SPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-hLOV-TEVcs-Gal4
Plasmid#173120PurposeExpresses CCR6 hLOV receiver construct in DIPGDepositorInsertCCR6-hLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV CMV CCR6-eLOV-TEVcs-Gal4
Plasmid#173113PurposeExpresses CCR6 receiver construct in mammalian cellsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-GFP::NMN-Deamidase
Plasmid#187868PurposeGal4/UAS expression of GFP::NMN-DeamidaseDepositorInsertNMN-Deamidase
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFPO-His-G-Ca-FLITS
Plasmid#191455PurposeBacterial isolation of genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTagsHisExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGPN
Plasmid#187482PurposeExpression vector - GFPDepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-SNAP-MD-OCT4
Plasmid#115691PurposeConstitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a mitotic degron (MD)DepositorInsertSNAP-MD-OCT4
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK C-term
Plasmid#186147PurposeFor expresseion of recombinant FAK c-terminal domain in E. coliDepositorInsertPTK2 (PTK2 Mouse)
UseTagsHis6-sumoExpressionBacterialMutationPromoterT7Available sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE-RARa-VP16
Plasmid#185561PurposeDoxycycline inducible expression of constitutively active Retinoic Acid Receptor alphaDepositorInsertRARa-VP16 (Rara Mouse)
UseTagsVP16ExpressionMammalianMutationPromoterTREAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8291 MSCV-Neo C-HA 18E7 ΔDLLC
Plasmid#184523PurposeExpression of HPV18 E7 ΔDLLCDepositorInsertHPV18 E7 ΔDLLC
UseRetroviralTagsHAExpressionMutationdeletion of DLLCPromoterMSCV LTRAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pb mD3S4-dN
Plasmid#180252PurposeP. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.DepositorInsertD3 of P. berghei HAP2 with N-glycosylation sites mutated
UseTags6HisExpressionMammalianMutationN516T, S533N and N539QPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_10bp 5'SS_No BP or pBP_pUC18
Plasmid#100989PurposeRp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationtGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 Y635A
Plasmid#175772PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635ADepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationY635APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 N637A
Plasmid#175773PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637ADepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationN637APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
NmMetNI-WT
Plasmid#172114PurposeExpression of MetN and MetI membrane proteinsDepositorInsertsMetN
MetI
UseTags10x His Tag and EnterokinaseExpressionBacterialMutationPromoterT7 PromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dR108E-cs-TEV-STREP
Plasmid#171835PurposeMammalian expression of human WIPI2d R108E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationR108EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dR125E-cs-TEV-STREP
Plasmid#171836PurposeMammalian expression of human WIPI2d R125E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationR125EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dK128E-cs-TEV-STREP
Plasmid#171837PurposeMammalian expression of human WIPI2d K128E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationK128EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dH85E-cs-TEV-STREP
Plasmid#171831PurposeMammalian expression of human WIPI2d H85E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationH85EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dK88E-cs-TEV-STREP
Plasmid#171832PurposeMammalian expression of human WIPI2d K88E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationK88EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI2dI92E-cs-TEV-STREP
Plasmid#171833PurposeMammalian expression of human WIPI2d I92E with C-terminal StrepDepositorInsertWIPI2d (WIPI2 Human)
UseTagsStrepExpressionMammalianMutationI92EPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only