-
Plasmid#222076PurposeeforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pVP074
Plasmid#222077PurposeeforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP075
Plasmid#222078PurposeAeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.DepositorTypeEmpty backboneUseChromoprotein-marked pvs1 backboneTagsExpressionMutationPromoterAvailable sinceJan. 27, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
5T5G (SETD8)
Plasmid#101886PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETD8 (KMT5A Human)
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
UseTagsExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available sinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLO87
Plasmid#91726Purposemycobacterial expression of mCherry from Hsp60 promoterDepositorInserthsp60 promoter
UseMycobacterial expressionTagsmCherryExpressionMutationPromoterhsp60Available sinceMay 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG75-β2AR-T4L-sfGFP
Plasmid#102461PurposeExpresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ2AR
UseTagsHis tag and sfGFPExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSG74-β2AR-sfGFP
Plasmid#102460PurposeExpresses a fusion protein of wild type β2 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ2AR
UseTagsHis tag and sfGFPExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p201N 1509
Plasmid#55768PurposeContains soybean miRNA miR1509 recognition sequence (CAACCTTGATTTCCTTGATTAA) to produce siRNAs from 3' target sequences and induce RNA silencingDepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterGmUbiAvailable sinceSept. 8, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJP_Ctrl10
Plasmid#219672PurposeContains PT3lacO promoter (regulated by blue light and LacI) controlling the expression of mCherry. LacI is constitutively produced by pR promoter.DepositorInsertsmCherry
lacI
UseTagsExpressionMutationPromoterPT3lacO and pRAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG73-β1ARts-sfGFP
Plasmid#102459PurposeExpresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ1AR
UseTagsHis tag and sfGFPExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
p201N 1510a.2
Plasmid#55770PurposeContains soybean miRNA miR1510a.2 recognition sequence (ATGGGTGGAATAGGGAAAACAA) to produce siRNAs from 3' target sequences and induce RNA silencingDepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterGmUbiAvailable sinceOct. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p201N 5770
Plasmid#55772PurposeContains soybean miRNA miR5770 recognition sequence (TCTTGTCCAAACCATAGTCCAA) to produce siRNAs from 3' target sequences and induce RNA silencingDepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterGmUbiAvailable sinceSept. 26, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p201N 1510
Plasmid#55771PurposeContains soybean miRNA miR1510 recognition sequence (AGGTGGAATAGGAAAAACAACT) to produce siRNAs from 3' target sequences and induce RNA silencingDepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterGmUbiAvailable sinceSept. 26, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
p201N 3514
Plasmid#55769PurposeContains soybean miRNA miR3514 recognition sequence (AAGGTCTCTGTCTTAATGGTGA) to produce siRNAs from 3' target sequences and induce RNA silencingDepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterGmUbiAvailable sinceAug. 21, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP143
Plasmid#222080PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.DepositorInsertdCas9-PmCDA1, sfGFP, sacB and AeBlue
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVP141
Plasmid#222079PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.DepositorInsertdCas9-PmCDA1, sfGFP and sacB
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVP144
Plasmid#222081PurposeSingle component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.DepositorInsertdCas9-PmCDA1, sfGFP, sacB and eforRED
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
ILID-CAAX
Plasmid#85680PurposeExpression of untagged ILID CAAXDepositorInsertILID-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
p201G Cas9
Plasmid#59178PurposeCas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
sGFP
UseCRISPRTagsExpressionMutationPromoter2x35SAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR2
Plasmid#64932PurposeEnhanced FRET-based biosensor for monitoring Akt activity.DepositorInsertAktAR2
UseTagsCerulean3 and cpVenus[E172]ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
p201B Cas9
Plasmid#59177PurposeCas9 driven by double 35S, BAR for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
BAR
UseCRISPRTagsExpressionMutationPromoter2x35SAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
MCS+
Plasmid#90412PurposeA FRET sensor that report the electric potential at the inner leaflet of cell plasma membraneDepositorInsertLck10+ Venus+mcherry+ strech of positively charged amino acids
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCS Lck only
Plasmid#90411PurposeA control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.DepositorInsertLck10+ Venus+mcherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Venus-KRasCT
Plasmid#99016PurposeMembrane markerDepositorInsertVenus KRasCT
UseTagsExpressionMammalianMutationPromoterCMAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-KRasCT
Plasmid#99017PurposeMembrane markerDepositorInsertmCherry KRas CT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFBXC3GH
Plasmid#49051PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionInsectMutationPromoterpolH (polyhedrin)Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGT572_Nemo
Plasmid#153487PurposeLevel 1 episomal expression plasmid for S. cerevisiaeDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAP002
Plasmid#153489PurposeLevel 1 episomal expression plasmid for P. pastorisDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_GFP11
Plasmid#153508PurposeGFP11 C-terminal Tag Donor PlasmidDepositorInsertGFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBXC3H
Plasmid#49050PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionInsectMutationPromoterpolH (polyhedrin)Available sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFBXNH3
Plasmid#49049PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionInsectMutationPromoterpolH (polyhedrin)Available sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAL120-modified
Plasmid#22057DepositorInsertmCMV expression cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab4 CA
Plasmid#53466DepositorInsertRab04
UseTagsYFPExpressionInsectMutationQ67LPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAL120
Plasmid#22056DepositorInsertmCMV expression cassette
UseAdenoviralTagsExpressionMutationPromoterAvailable sinceNov. 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPAP001
Plasmid#153488PurposeLevel 1 episomal expression plasmid for P. pastorisDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PRC3MKO2
Plasmid#193757PurposeExpresses mKO2 under the control of PR promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertmKO2
UseTagsExpressionBacterialMutationPromoterPRAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVG97
Plasmid#164849PurposePlasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t constructDepositorInsertPart of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_TwinStrep
Plasmid#153513PurposeTwinStrep C-terminal Tag Donor PlasmidDepositorInsertTwinStrep
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rabx5 WT
Plasmid#53494DepositorInsertRabx5
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rabx2 DN
Plasmid#53475DepositorInsertRabx2
UseTagsYFPExpressionInsectMutationS21NPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-SP6
Plasmid#102418PurposeAc transposase with SP6 promoter for in vitro transcriptionDepositorInsertAc transposase
UseTagsExpressionBacterialMutationPromoterSP6Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-MCS-Ds
Plasmid#102416PurposeEmpty vector with a multiple cloning site flanked by Ds integration elementsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP-150TAG
Plasmid#174076PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,DepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationN150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRBC-sfGFP wild type
Plasmid#174075PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationPromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORF1-Flag
Plasmid#190565PurposeMammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.DepositorInsertORF1 (from L1.3)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-mgSrtA
Plasmid#125792PurposeTransfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.DepositorInsertmgSrtA
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
p201H Cas9
Plasmid#59176PurposeCas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
hph
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_sfGFP1-10
Plasmid#153515PurposeE.coli expression plasmid for the production of GFP1-10DepositorInsertsfGFP1-10
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only