Skip to main content
Addgene
Showing: 1401 - 1450 of 4068 results
  1. pVP073

    Plasmid
    #222076
    Purpose
    eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pVP074

    Plasmid
    #222077
    Purpose
    eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pVP075

    Plasmid
    #222078
    Purpose
    AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.
    Depositor
    Type
    Empty backbone
    Use
    Chromoprotein-marked pvs1 backbone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. 5T5G (SETD8)

    Plasmid
    #101886
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    SETD8 (KMT5A Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pSG75-β2AR-T4L-sfGFP

    Plasmid
    #102461
    Purpose
    Expresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Insert
    β2AR
    Use
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pSG74-β2AR-sfGFP

    Plasmid
    #102460
    Purpose
    Expresses a fusion protein of wild type β2 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Insert
    β2AR
    Use
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. p201N 1509

    Plasmid
    #55768
    Purpose
    Contains soybean miRNA miR1509 recognition sequence (CAACCTTGATTTCCTTGATTAA) to produce siRNAs from 3' target sequences and induce RNA silencing
    Depositor
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    GmUbi
    Available since
    Sept. 8, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pJP_Ctrl10

    Plasmid
    #219672
    Purpose
    Contains PT3lacO promoter (regulated by blue light and LacI) controlling the expression of mCherry. LacI is constitutively produced by pR promoter.
    Depositor
    Inserts
    mCherry
    lacI
    Use
    Tags
    Expression
    Mutation
    Promoter
    PT3lacO and pR
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pSG73-β1ARts-sfGFP

    Plasmid
    #102459
    Purpose
    Expresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Insert
    β1AR
    Use
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. p201N 1510a.2

    Plasmid
    #55770
    Purpose
    Contains soybean miRNA miR1510a.2 recognition sequence (ATGGGTGGAATAGGGAAAACAA) to produce siRNAs from 3' target sequences and induce RNA silencing
    Depositor
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    GmUbi
    Available since
    Oct. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. p201N 5770

    Plasmid
    #55772
    Purpose
    Contains soybean miRNA miR5770 recognition sequence (TCTTGTCCAAACCATAGTCCAA) to produce siRNAs from 3' target sequences and induce RNA silencing
    Depositor
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    GmUbi
    Available since
    Sept. 26, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. p201N 1510

    Plasmid
    #55771
    Purpose
    Contains soybean miRNA miR1510 recognition sequence (AGGTGGAATAGGAAAAACAACT) to produce siRNAs from 3' target sequences and induce RNA silencing
    Depositor
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    GmUbi
    Available since
    Sept. 26, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  13. p201N 3514

    Plasmid
    #55769
    Purpose
    Contains soybean miRNA miR3514 recognition sequence (AAGGTCTCTGTCTTAATGGTGA) to produce siRNAs from 3' target sequences and induce RNA silencing
    Depositor
    Type
    Empty backbone
    Use
    RNAi
    Tags
    Expression
    Mutation
    Promoter
    GmUbi
    Available since
    Aug. 21, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  14. pVP143

    Plasmid
    #222080
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.
    Depositor
    Insert
    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pVP141

    Plasmid
    #222079
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion.
    Depositor
    Insert
    dCas9-PmCDA1, sfGFP and sacB
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. pVP144

    Plasmid
    #222081
    Purpose
    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.
    Depositor
    Insert
    dCas9-PmCDA1, sfGFP, sacB and eforRED
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  17. MCS+

    Plasmid
    #90412
    Purpose
    A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane
    Depositor
    Insert
    Lck10+ Venus+mcherry+ strech of positively charged amino acids
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  18. MCS Lck only

    Plasmid
    #90411
    Purpose
    A control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.
    Depositor
    Insert
    Lck10+ Venus+mcherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  19. pVG97

    Plasmid
    #164849
    Purpose
    Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct
    Depositor
    Insert
    Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. pRBC-sfGFP-150TAG

    Plasmid
    #174076
    Purpose
    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,
    Depositor
    Insert
    Superfolder GFP
    Use
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    T7
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pORF1-Flag

    Plasmid
    #190565
    Purpose
    Mammalian expression plasmid encoding the first open reading frame (ORF1, expressing the protein ORF1p) of human L1 (L1.3); carries a C-terminus Flag tag.
    Depositor
    Insert
    ORF1 (from L1.3)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  22. pDisplay-mgSrtA

    Plasmid
    #125792
    Purpose
    Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.
    Depositor
    Insert
    mgSrtA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1401 - 1450 of 4068 results