We narrowed to 12,952 results for: sequence

1. pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

   Plasmid

   #183607

   Purpose

   Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   LaG17 GFP nanobody SynNotch tTA
   

   Use

   Tags

   Myc and human CD8a signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Pgk1

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-FLAG-Sestrin1 isoform1

   Plasmid

   #72594

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN1 (SESN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLXSN IkB alpha M

   Plasmid

   #12330

   Depositor

   Inder Verma

   Article

   Van Antwerp et al Science. 1996 Nov 1. 274(5288):787-9.

   Insert

   IkB alpha M (Nfkbia Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Dominant-negative mutant. Many signal transductio…

   Promoter

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-MCS-eDHFR(69K6)-EGFP

   Plasmid

   #172101

   Purpose

   Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   22aa-eDHFR(69K6)-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

   Plasmid

   #213034

   Purpose

   fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Slaviero et al Neurophotonics. 2024 Apr;11(2):024208. d

   Insert

   mNeonGreen, ISN-IE, ChRmine
   

   Use

   AAV

   Tags

   Gaussia luciferase signal sequence

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXZX501

   Plasmid

   #169455

   Purpose

   pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

   Depositor

   Jef Boeke

   Article

   Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

   Insert

   gRNA for MATa
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Plxnb1(L)-Fc-His

   Plasmid

   #72126

   Purpose

   Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxnb1 (Plxnb1 Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

   Plasmid

   #115789

   Purpose

   Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   NDP (NDP Human)
   

   Use

   Tags

   alkaline phosphatase and flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXZX351

   Plasmid

   #169454

   Purpose

   pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

   Depositor

   Jef Boeke

   Article

   Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

   Insert

   gRNA for MATα
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF-Ivy-his-hLYZ

    Plasmid

    #83920

    Purpose

    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Ivy
    lysozyme (LYZ Human)
    

    Use

    Tags

    Ivy protein has C-terminal hisx6 tag

    Expression

    Bacterial

    Mutation

    Ivy leader sequence deleted and replaced.

    Promoter

    T7 and t7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-CCR5

    Plasmid

    #98948

    Purpose

    Mammalian expression plasmid for N-terminal myc-tagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRP6-m5-pCS2-VSVG

    Plasmid

    #27283

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Expression

    Mutation

    Changed S/T of all five PPP(S/T)P motifs to Alani…

    Promoter

    Available Since

    March 3, 2011

    Availability

    Academic Institutions and Nonprofits only

13. Sema4g-Fc-His

    Plasmid

    #72160

    Purpose

    Expresses the extracellular region of the Sema4G protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4g (Sema4g Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBXNPH3

    Plasmid

    #110098

    Purpose

    E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-FLAG-Sestrin3

    Plasmid

    #72592

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN3 (SESN3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

16. Sema3g.a(L)-Fc-His

    Plasmid

    #72151

    Purpose

    Expresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3g.a (Sema3g Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT (AF20)

    Plasmid

    #21359

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available Since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

    Plasmid

    #176277

    Purpose

    Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    MCS-P2A-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-7aa-ActA-pEGFP-C1

    Plasmid

    #177406

    Purpose

    To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

    Depositor

    David Andrews

    Article

    Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

    Insert

    ActA tail anchor (actA Listeria monocytogenes)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Plxna2-AP-His

    Plasmid

    #71997

    Purpose

    Expresses the extracellular region of the PlexinA2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna2 (Plxna2 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET20b_Pa-PirA

    Plasmid

    #128956

    Purpose

    expresses Pseudomonas aeruginosa PirA in E. coli

    Depositor

    James Naismith

    Article

    Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

    Insert

    ferric enterobactin receptor PirA (pirA Synthetic, Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    6xHis and pelB leader

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    T7

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. AAV_pSyn_Mito-LOV-Turbo-V5

    Plasmid

    #199669

    Purpose

    expresses Mito-LOV-Turbo-V5 in neurons, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    AAV

    Tags

    COX4I1 mito targeting sequence and V5

    Expression

    Mutation

    Promoter

    Synapsin

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-CaMKII-soCoChR-GFP

    Plasmid

    #107711

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. Human _TFAM_NoMTS_Dimer_pET28a+

    Plasmid

    #60013

    Purpose

    Expresses TFAM No MTS dimerization mutant in bacterial cells

    Depositor

    David Chan

    Article

    Ngo et al Nat Commun. 2014;5:3077. doi: 10.1038/nc

    Insert

    Human TFAM No MTS (TFAM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Missing 1st 42 aas (Mitochondrial targeting seque…

    Promoter

    T7

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. gRNA_DNMT3a-T1

    Plasmid

    #41821

    Purpose

    Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_DNMT3a-T1 (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. pENTR4-EGFP_opt

    Plasmid

    #106334

    Purpose

    Gateway Entry vector for EGFP (codon optimized for efficient gene synthesis)

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    codon optimized for gene synthesis without affect…

    Promoter

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDONR221_SLC39A8

    Plasmid

    #132177

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A8 (SLC39A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pFastBacDual with LFn + Needle

    Plasmid

    #84868

    Purpose

    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Needle
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. pcDNA3.1-FAM20C:V5

    Plasmid

    #63584

    Purpose

    FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.

    Depositor

    Kenneth Irvine

    Article

    Ishikawa et al PLoS One. 2012;7(8):e42988. doi: 10.1371

    Insert

    FAM20C (Fam20c Mouse)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pEGIP*35

    Plasmid

    #26776

    Purpose

    Enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP*35
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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