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Showing: 8761 - 8790 of 21422 results

  3. pZH521

    Plasmid
    #102674
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102674-plasmid-map-sequence-id-199585

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  6. pMito-dCherry-FRB

    Plasmid
    #186573
    Purpose
    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells
    Depositor
    Stephen Royle
    Article
    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:
    Insert
    Dark MitoTrap
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutation of "K70N" to render mCherry no…
    Promoter
    CMV
    Available since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    186573-plasmid-map-sequence-id-368442

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  8. pTF-ahpC-rfp

    Plasmid
    #153035
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153035-plasmid-map-sequence-id-318838

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  14. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  19. pTJV1Sc2-tetA

    Plasmid
    #166982
    Purpose
    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    CspRecT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pVanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166982-plasmid-map-sequence-id-331335

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  20. pJYDN3p

    Plasmid
    #162457
    Purpose
    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162457-plasmid-map-sequence-id-321472

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Showing: 8761 - 8790 of 21422 results