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20,205 results

Showing: 11921 - 11960 of 20205 results

  6. AAV-KrasG12D/sgKras/Cre

    Plasmid
    #99851
    Purpose
    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
    Depositor
    Monte Winslow
    Article
    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:
    Insert
    Kras (Kras Mouse)
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Mutation
    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…
    Available Since
    Dec. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99851-plasmid-map-sequence-id-193760

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  28. pAluYa5-neo-TET

    Plasmid
    #51283
    Purpose
    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    AluYa5
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Promoter
    upstream pol III enhancer sequence of the 7SL gen…
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51283-plasmid-map-sequence-id-78362

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  38. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Article
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112139-plasmid-map-sequence-id-217823

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  39. pFLAG-CMV-hErk1(K71R)

    Plasmid
    #49329
    Purpose
    Expresses kinase deficient FLAG-tagged human Erk1
    Depositor
    Melanie Cobb
    Insert
    Erk1 (MAPK3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    K71R
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pcDNA5/FRT/TO-GAlphasL-RLuc8

    Plasmid
    #140981
    Purpose
    Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasL-RLuc8 (GNAS Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140981-plasmid-map-sequence-id-300681

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Showing: 11921 - 11960 of 20205 results