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Showing: 101 - 150 of 242 results

  7. pBactin-AcGFP-Cdk5rap2-CTD

    Plasmid
    #196853
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTD (Cdk5rap2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196853-plasmid-map-sequence-id-390738

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  10. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Use
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pTRE3G-Bi-gePSI-pCamk2a-AcGFP

    Plasmid
    #133732
    Purpose
    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.
    Depositor
    Erin Schuman
    Article
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Inserts
    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    Use
    AAV
    Tags
    murine ornithine decarboxylase - degron (ODC36)
    Expression
    Mammalian
    Mutation
    Promoter
    pCamk2a and pTRE3G-Bi
    Available since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    133732-plasmid-map-sequence-id-266808

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  17. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid
    #196856
    Purpose
    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Lacking the centrosome-targeting domain (CTD)
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196856-plasmid-map-sequence-id-391815

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  29. Danio alpha A-crystallin 1kb promoter/AcGFP

    Plasmid
    #98096
    Purpose
    aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha A-crystallin 1 kb fragment (-1028/-1)
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98096-plasmid-map-sequence-id-193844

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  31. Danio alpha Bb-crystallin 1kb promoter/AcGFP

    Plasmid
    #98098
    Purpose
    aBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio aBb-crystallin 1 kb fragment (-1074/-1)
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98098-plasmid-map-sequence-id-193846

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  32. Danio alpha Ba-crystallin 3kb promoter/AcGFP

    Plasmid
    #98097
    Purpose
    aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98097-plasmid-map-sequence-id-193845

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  33. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid
    #91803
    Purpose
    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used
    Depositor
    Jeremy Wilusz
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    91803-plasmid-map-sequence-id-180321

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  34. Zebrafish aBb crystallin 5kb promoter/AcGFP

    Plasmid
    #98101
    Purpose
    Zebrafish aBb crystallin 5kb promoter segment driving GFP expression
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha Bb-crystallin promoter 5 kb fragment, Danio rerio (cryabb Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha Bb-crystallin 5 kb promoter fragment …
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98101-plasmid-map-sequence-id-193849

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  35. Zebrafish aBb crystallin 4kb promoter/AcGFP

    Plasmid
    #98100
    Purpose
    Zebrafish aBb crystallin 4kb promoter segment driving GFP expression
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha Bb-crystallin promoter 4 kb fragment, Danio rerio (cryabb Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha Bb-crystallin 4 kb promoter fragment …
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98100-plasmid-map-sequence-id-193848

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  36. Zebrafish aBb crystallin 2kb promoter/AcGFP

    Plasmid
    #98099
    Purpose
    Zebrafish aBb crystallin 2kb promoter segment driving GFP expression
    Depositor
    Mason Posner
    Article
    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717
    Insert
    Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)
    Use
    Gfp expressing
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    98099-plasmid-map-sequence-id-193847

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  46. pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

    Plasmid
    #228398
    Purpose
    Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    NLS/cMyc-AcGFP2x-NES/iKB2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228398-plasmid-map-sequence-id-457132

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  47. pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

    Plasmid
    #228399
    Purpose
    Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm
    Depositor
    Dirk Daelemans
    Article
    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:
    Insert
    3xFLAG-NLS/SV40-AcGFP-NES/PKI
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228399-plasmid-map-sequence-id-457131

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Showing: 101 - 150 of 242 results