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pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
3537 pMIG Bim

Plasmid

#8786

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bim (Bcl2l11 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pSpCas9n(BB)-2A-GFP (PX461)

Plasmid

#48140

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-MitoTimer

Plasmid

#50547

Purpose

Expresses the Timer protein targeted to the mitochondria under a Tet-inducible promoter

Depositor

Roberta Gottlieb

Article

Hernandez et al Autophagy. 2013 Nov 1;9(11):1852-61. doi

Insert

MitoTimer

Expression

Mammalian

Mutation

F12S mutation in Timer

Available Since

March 14, 2014

Availability

Academic Institutions and Nonprofits only

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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Lamp1-RFP

Plasmid

#1817

Purpose

Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

lysosome associated membrane protein 1 (Lamp1 Rat)

Tags

RFP

Expression

Mammalian

Mutation

D50E (not important for function of plasmid)

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
Lact-C2-GFP

Plasmid

#22852

Depositor

Sergio Grinstein

Article

Yeung et al Science. 2008 Jan 11. 319(5860):210-3.

Insert

Lactadherin-C2 Domain (MFGE8 Bovine)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pMSP2N2

Plasmid

#29520

Depositor

Stephen Sligar

Article

Grinkova et al Protein Eng Des Sel. 2010 Nov . 23(11):8

Insert

MSP2N2

Tags

7-His tag

Expression

Bacterial

Mutation

tandem MSP; His-tag on N-terminus followed by spa…

Available Since

May 3, 2011

Availability

Academic Institutions and Nonprofits only

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EMTB-3XGFP

Plasmid

#26741

Depositor

William Bement

Article

Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

Insert

ensconsin (MAP7 Human)

Use

Probe for microtubules

Tags

3x EGFP and Myc

Expression

Mammalian

Mutation

Only contains the microtubule binding-domain of e…

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pMSP1D1

Plasmid

#20061

Purpose

Bacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formation

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1D1

Tags

7-His tag

Expression

Bacterial

Mutation

deletion mutant (1-11) of MSP1; His-tag on N-term…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0

Plasmid

#62987

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-GFP-NMHC II-A

Plasmid

#11347

Depositor

Robert Adelstein

Article

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-A (MYH9 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pET28b-DiCas7-11-His

Plasmid

#234482

Purpose

Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DiCas7-11

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-F-del Casp9.IRES.GFP

Plasmid

#15567

Depositor

David Spencer

Article

Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

Insert

FKBP12(V36)-p30Caspase9 (FKBP1A Human)

Use

Retroviral

Tags

FKBP12 and HA epitope

Expression

Mammalian

Mutation

FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

Available Since

Aug. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR

Plasmid

#13507

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 WT

Plasmid

#16233

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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SIRT3 Flag

Plasmid

#13814

Purpose

Mammalian expression of human SIRT3 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT3 (SIRT3 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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