We narrowed to 5,795 results for: plasmid dna

1. pLV-mCherry

   Plasmid

   #36084

   Purpose

   3rd generation lentiviral plasmid expressing mCherry

   Depositor

   Pantelis Tsoulfas

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR4-HaloTag (w876-1)

   Plasmid

   #29644

   Depositor

   Eric Campeau

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available Since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAPE

   Plasmid

   #217451

   Purpose

   For digestion with restriction enzymes to create DNA size marker ladders

   Depositor

   Michael Lardelli

   Article

   Allen et al Biol Methods Protoc. 2023 Jul 31;8(1):bp

   Type

   Empty backbone

   Use

   Dna size markers

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPSU1

   Plasmid

   #89439

   Purpose

   High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders

   Depositor

   Song Tan

   Article

   Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

   Inserts

   500 bp EcoRV fragment
   1000 bp EcoRV fragment
   1500 bp EcoRV fragment
   2000 bp EcoRV fragment
   500 bp PstI fragment
   700 bp PstI fragment
   800 bp PstI fragment (EHMT2 Human)
   900 bp PstI fragment
   1000 bp PstI fragment (BMI1 Human)
   2000 bp PstI fragment
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRRLSIN.cPPT.PGK-GFP.WPRE

   Plasmid

   #12252

   Purpose

   3rd generation lentiviral backbone

   Depositor

   Didier Trono

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMT-puro

   Plasmid

   #17923

   Purpose

   Insect expression vector

   Depositor

   David Sabatini

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Drosophila metallothionein gene promoter drives e…

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP.Cre empty vector

   Plasmid

   #20781

   Depositor

   Tyler Jacks

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

   Plasmid

   #178320

   Purpose

   Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

   Inserts

   HRE RedFirefly reporter
   P53 FLuc reporter
   AP-1 Renilla reporter
   FOXO NLuc Reporter
   SMAD GrRenilla reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSCV Luciferase PGK-hygro

   Plasmid

   #18782

   Depositor

   Scott Lowe

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TB:4xHRE_RE:MiniP

    Plasmid

    #178313

    Purpose

    4 copies of the HRE DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    4 copies of the heat shock DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mini-pHelper-1.0

    Plasmid

    #230933

    Purpose

    The mini verison (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

    Depositor

    Qizhao Wang

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAVone-AAV9-CMV-eGFP

    Plasmid

    #230928

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAVone-AAV2-CMV-eGFP

    Plasmid

    #230930

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV2 serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLSLR

    Plasmid

    #51500

    Purpose

    Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Plant t-dna plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAS007

    Plasmid

    #164868

    Purpose

    This plasmid can be used to study DNA looping in vivo. pAS007 contains the phiKO2 cB operator sequences but can be modified to insert any DNA operators of interest.

    Depositor

    Keith Shearwin

    Article

    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

    Insert

    phiKO2 operators
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAVone-AAV-PHP.eB-CMV-eGFP

    Plasmid

    #230929

    Purpose

    pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV-PHP.eB serotype, utilizing the AAVone single-plasmid AAV production system.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCV-IRES-mCherry FP

    Plasmid

    #52114

    Purpose

    a bicistronic IRES-mCherry containing retroviral vector

    Depositor

    Dario Vignali

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabe puro IRES-EGFP

    Plasmid

    #14430

    Depositor

    L. Miguel Martins

    Has Service

    Cloning Grade DNA

    Insert

    IRES-EGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-SFFV-Emerald-WPRE-UbC-mCherry

    Plasmid

    #211802

    Purpose

    Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    Emerald
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-SFFV-mCherry-WPRE-UbC-Emerald

    Plasmid

    #211797

    Purpose

    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    mCherry
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLSL

    Plasmid

    #51493

    Purpose

    T-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Plant t-dna plasmid

    Tags

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAVtri-CMV-eGFP

    Plasmid

    #230932

    Purpose

    Used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 3xDBE_RE:NLuc

    Plasmid

    #178318

    Purpose

    FOXO NLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    FOXO NLuc Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 4xHRE_RE:RedF

    Plasmid

    #178314

    Purpose

    HRE RedFirefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    HRE RedFirefly reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-SFFV-MCS-WPRE-UbC-mCherry

    Plasmid

    #211799

    Purpose

    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    multiple cloning site
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-SFFV-mCherry-WPRE-UbC-MCS

    Plasmid

    #211801

    Purpose

    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    mCherry
    multiple cloning site
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-SFFV-MCS-WPRE-UbC-MCS

    Plasmid

    #211798

    Purpose

    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and another multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    multiple cloning site
    multiple cloning site
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-SFFV-MCS-WPRE-UbC-Emerald

    Plasmid

    #211796

    Purpose

    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    multiple cloning site
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-SFFV-Emerald-WPRE-UbC-MCS

    Plasmid

    #211800

    Purpose

    Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    Emerald
    multiple cloning site
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pWPI

    Plasmid

    #12254

    Purpose

    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.

    Depositor

    Didier Trono

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    EMCV IRES-EGFP cassette

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUC119

    Plasmid

    #50010

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Vieira et al Methods Enzymol. 1987;153:3-11.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUC118

    Plasmid

    #50009

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Vieira et al Methods Enzymol. 1987;153:3-11.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 2xP53_RE::FLuc

    Plasmid

    #178316

    Purpose

    P53 Firefly luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    P53 FLuc Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. 6xAP-1_RE::Renilla

    Plasmid

    #178317

    Purpose

    AP-1 Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    AP-1 Renilla Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 7xSMAD_RE::GrRenilla

    Plasmid

    #178319

    Purpose

    SMAD GreenRenilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Insert

    SMAD GreenRenilla Reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAVdual-CMV-eGFP

    Plasmid

    #230931

    Purpose

    pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAIP4_1AKZ

    Plasmid

    #234172

    Purpose

    Heterologous protein expression of uracil-DNA glycosylase (UDG) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    1AKZ (UNG Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 10, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. IpO

    Plasmid

    #48233

    Purpose

    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    

    Use

    Routine cloning vector

    Tags

    Expression

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    URA3 promoter and none

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pOpen-RNAse A

    Plasmid

    #165565

    Purpose

    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    RNAse A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pLSLGFP

    Plasmid

    #51494

    Purpose

    pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    GFP
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    NLS and flag

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLSLT

    Plasmid

    #51498

    Purpose

    pLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    T30 TALEN pair
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSpCas9_T2A_mCherry sg1-sg2

    Plasmid

    #192479

    Purpose

    All-in-one plasmid containing both guides for cutting BFP region in DSB Spectrum V1

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSpCas9_T2A_mCherry sgBFP

    Plasmid

    #192929

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pSpCas9_T2A_iRFP670 sgAAVS1

    Plasmid

    #192477

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSpCas9_T2A_iRFP670 sgBFP

    Plasmid

    #192478

    Purpose

    All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Use

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. AAV-SYN-PIK3CD

    Plasmid

    #203730

    Purpose

    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid

    #89180

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLSLGUS

    Plasmid

    #51517

    Purpose

    pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    GUS
    3kb filler sequence
    

    Use

    Plant t-dna plasmid

    Tags

    NLS and flag

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLSLZ.D

    Plasmid

    #51496

    Purpose

    pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    us:NPTII repair template
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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