178,218 results

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pYLsgRNA-AtU3d

Plasmid

#66200

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Promoter

AtU3d

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pFUGW-hGtACR1-EYFP

Plasmid

#67795

Purpose

Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

Depositor

John Spudich

Publication

Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Insert

hGtACR1

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Publication

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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GFP-GCN5

Plasmid

#65386

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GCN5 (KAT2A Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pU6a:sgRNA#2

Plasmid

#64246

Purpose

expresses sgRNA under U6a promoter

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCAG-VSVG

Plasmid

#64084

Purpose

Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene

Depositor

Ian Wickersham

Insert

VSV glycoprotein

Expression

Mammalian

Promoter

CAG

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pPtPuc3

Plasmid

#62863

Purpose

Episome vector for Phaeodactylum tricornutum (pUC-based)

Depositor

Hamilton Smith

Publication

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for phaeodacty…

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGL3-Basic-8x-gRNA-eGFP

Plasmid

#60718

Purpose

eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

Depositor

Charles Gersbach

Publication

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCS2-Tol1.zf1

Plasmid

#61388

Purpose

mRNA synthesis of tol1 transposase codon modified for zebrafish

Depositor

Harold Burgess

Publication

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

tol1.zf1

Use

Zebrafish expression

Promoter

sp6

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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single polypeptide FPX biosensor for calcium ion

Plasmid

#60887

Purpose

FPX biosensor

Depositor

Robert Campbell

Publication

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

single polypeptide FPX biosensor for calcium ion

Tags

HindIII site

Expression

Mammalian

Promoter

CMV

Available since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pX330S-4

Plasmid

#58780

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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tol2-mpeg1-mcherry

Plasmid

#58935

Purpose

labels macrophages with mcherry

Depositor

Anna Huttenlocher

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mpeg1-mcherry

Promoter

mpeg1

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-C3-Exo70

Plasmid

#53761

Purpose

pEGFP-C3 plasmid that expresses Exo70

Depositor

Channing Der

Publication

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Exo70 (EXOC7 Human)

Tags

GFP

Expression

Mammalian

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCS-H2B-EGFP

Plasmid

#53744

Purpose

Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

Depositor

Sean Megason

Publication

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

histone H2B (H2BC21 Human)

Tags

EGFP

Expression

Mammalian

Promoter

simian CMV IE94

Available since

June 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.1 3xFlag IFIT1

Plasmid

#53554

Purpose

mammalian expression of IFIT1

Depositor

Kathleen Collins

Publication

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT1 (IFIT1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCDNA-3xFLAG-Pontin

Plasmid

#51635

Purpose

Mammalian expression of 3xFLAG tagged pontin, N-terminal.

Depositor

Steven Artandi

Publication

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

Pontin (RUVBL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

Plasmid

#52421

Purpose

Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

Depositor

Bertrand Collet

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fusion NLS-EGFP-P2A-Cherry-CAAX

Expression

Mammalian

Promoter

CMV

Available since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Publication

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Nano-lantern

Expression

Mammalian

Mutation

S257G in Rluc8

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA-HA-ER Y537S

Plasmid

#49499

Purpose

Expresses HA-tagged ER Y537S mutant in mammalian cells

Depositor

Sarat Chandarlapaty

Publication

Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

Insert

ESR1 (ESR1 Human)

Tags

HA

Expression

Mammalian

Mutation

Tyrosine 537 to Serine

Promoter

CMV

Available since

Dec. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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