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169,843 results

Showing: 9001 - 9030 of 169843 results

  1. pLenti-EB1-EGFP

    Plasmid
    #118084
    Purpose
    Expresses EB1-EGFP in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human EB1 (MAPRE1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Promoter
    EF1 alpha
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118084-plasmid-map-sequence-id-230385

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  4. FSHR-DuET

    Plasmid
    #213242
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FSHR (FSHR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213242-plasmid-map-sequence-id-430799

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  5. pHN764

    Plasmid
    #178819
    Purpose
    Expression of EndoH in E. coli
    Depositor
    Kazuhiro Abe
    Insert
    synthetic gene encoding endo-β-N-acetylglucosaminidase H
    Tags
    HIs8 and MBP
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178819-plasmid-map-sequence-id-351934

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  7. TFORF0626

    Plasmid
    #142279
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPZ (CEBPZ Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    142279-plasmid-map-sequence-id-427298

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  10. Sniper2L

    Plasmid
    #193856
    Purpose
    Expresses human codon-optimized Sniper2L and blasticidin resistance: EFS promoter-Sniper2L-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193856-plasmid-map-sequence-id-382455

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  13. pInt_attP1_sacB_kanR

    Plasmid
    #205301
    Purpose
    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205301-plasmid-map-sequence-id-404791

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  15. pJM483

    Plasmid
    #52987
    Purpose
    A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor
    Depositor
    Ding Xue
    Article
    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d
    Insert
    Lactaherin (Mfge8 Mouse)
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    52987-plasmid-map-sequence-id-82563

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  26. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

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  27. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

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Showing: 9001 - 9030 of 169843 results