We narrowed to 7,687 results for: ALP

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pCellFree_G03 TNF

Plasmid

#67062

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TNF (TNF Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pEGB Pnos:Luciferase:Tnos (GB0112)

Plasmid

#68217

Purpose

Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mAkt-ER

Plasmid

#39530

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt (Akt1 Mouse)

Tags

ER

Expression

Mammalian

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-Cx43-7

Plasmid

#57787

Purpose

Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Cx43

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Par6 delta 250-295 Flag tagged

Plasmid

#24646

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Par6c (Pard6a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the PDZ domain of Par6c (aa 250-295)

Available Since

June 2, 2010

Availability

Industry, Academic Institutions, and Nonprofits
deltaPYD-NLRP11.MYC

Plasmid

#131197

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

deltaPYD-NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

deletion of the pyrin domain (amino acids 1-124 m…

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/3xmyc_NLRP11-LRR

Plasmid

#131200

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

LRR of NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

contains only the leucine-rich repeats (LRRs), am…

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt(R25C)

Plasmid

#39532

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (Akt1 Mouse)

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

R25C

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
PKG I beta CAT

Plasmid

#16396

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 beta (PRKG1 Human)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
Man1B1-Myc

Plasmid

#163645

Purpose

Expresses myc tagged Man1B1 in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mannosidase alpha class 1B member 1 (MAN1B1 Human)

Tags

myc

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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D44_Ld_180090

Plasmid

#135858

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

alpha glucosidase II subunit, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3_HLA-cmyc-GRM3 C127A

Plasmid

#140451

Purpose

Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant

Depositor

Erik Procko

Insert

GRM3 (GRM3 Human)

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

Cysteine 127 to alanine

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:Lucia:bgH

Plasmid

#135293

Purpose

Transcriptional unit for constitutive expression of Lucia luciferase in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:Lucia:bGH

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDGB1omega2_SF (GB0466)

Plasmid

#68243

Purpose

Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEF1α-spider-monkey-retroCHMP3-HA

Plasmid

#154179

Purpose

expresses spider monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/3xmyc_NLRP11-NACHT

Plasmid

#131199

Purpose

mammalian expression

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NACHT of NLRP11 (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

contains only the NACHT domain (amino acids 125-5…

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK1-2/3

Plasmid

#91420

Purpose

Protein expression and purification of human SH3 domain construct NCK1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-2/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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JB95

Plasmid

#128922

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

nRasGD-BirA*-HA-MAO (NRAS Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1α-owl-monkey-retroCHMP3(148)-HA

Plasmid

#154252

Purpose

expresses prematurely truncated owl monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBGSA c-myc-FPR1

Plasmid

#62601

Purpose

Constitutive expression of c-myc-FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

Insert

FPR1 (FPR1 Human)

Tags

c-myc

Expression

Mammalian

Promoter

SRalpha

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

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