We narrowed to 29,795 results for: REP
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Plasmid#220209PurposeMammalian expression of wild type TEX264, C-terminal twin-Strep_HA tagDepositorInsertTEX264 (TEX264 Human)
UseTagsStrep-strep-HAExpressionMammalianMutationPromoterCMV/TetOAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102887PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102888PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-EGFP-3xHA (JDW 1322)
Plasmid#224494PurposeGateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)DepositorInsertLiefact-EGFP-3xHA
UseGateway cloningTagsExpressionMutationPromoterAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29b-3p
Plasmid#103394PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29b-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 myc-NL-GW
Plasmid#113446PurposeNanoLuc Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLuciferase; Gateway shuttle vectorTagscmyc-NanoLucExpressionMammalianMutationPromoterCMVAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-DYRK1B
Plasmid#101771PurposeMammalian expression of DYRK1B fusion proteinDepositorInsertDYRK1B / MIRK (DYRK1B Human)
UseTagsS peptide-flag-streptavidin binding peptide (SFB)ExpressionMammalianMutationsiRNA-resistant allelePromoterAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-181a-5p
Plasmid#103276PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-181a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 GW-NL-myc
Plasmid#113447PurposeNanoLuc Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseLuciferase; Gateway shuttle vectorTagsNanoLuc-cmycExpressionMammalianMutationPromoterCMVAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTR EE Clover
Plasmid#90239PurposeLentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (EE mutant)
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-941
Plasmid#103757PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-941 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-7-5p
Plasmid#103723PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-7-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti myc-NL-GW
Plasmid#113454PurposeLentiviral NanoLuc Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseLentiviral and LuciferaseTagscmyc-NanoLucExpressionMammalianMutationPromoterhUbCAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Tfap2c
Plasmid#128826Purposedox-dependent expression of mouse Tfap2cDepositorAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 GW-mCit-PA
Plasmid#113449PurposeProteinA-mCitrine Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseGateway shuttle vectorTagsmCitrine-ProteinAExpressionMammalianMutationPromoterCMVAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-101-3p
Plasmid#103165PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTR AE Clover
Plasmid#90240PurposeLentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (AE mutant)
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 PA-mCit-GW
Plasmid#113448PurposeProteinA-mCitrine Gateway shuttle vector for N-terminal fusionsDepositorTypeEmpty backboneUseGateway shuttle vectorTagsProteinA-mCitrineExpressionMammalianMutationPromoterCMVAvailable SinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-delta6xNHL-LIN-41
Plasmid#52722Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the 6xNHL repeats and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 593–868PromoterAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102890PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-516b-5p
Plasmid#103601PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-516b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-516b-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102892PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
UseTagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …PromoterAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102891PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Luc-Sox2-3'UTR
Plasmid#63895PurposeLuciferase reporter of mouse wildtype Sox2 3'UTRDepositorInsertSRY (sex determining region Y)-box 2 (Sox2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-138-5p
Plasmid#103233PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-138-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-138-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRTTO-NHASt-codoptUS2
Plasmid#135102PurposeCodon-optimized HSV-1 US2 with N-terminal HAStrep tagDepositorInsertHSV-1 US2 codon optimized
UseTagsHA-Strep-StrepExpressionMammalianMutationPromoterAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW-mCit-PA
Plasmid#113457PurposeLentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseLentiviralTagsmCitrine-ProteinAExpressionMammalianMutationPromoterhUbCAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19b-3p
Plasmid#103327PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19b-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only