162,717 results

1. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

   Plasmid

   #196702

   Purpose

   Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

   Insert

   human RNaseH1 wild type
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-S5E2-GFP-fGFP

   Plasmid

   #135631

   Purpose

   AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Has Service

   AAV PHP.eB, AAV1, and AAV9

   Insert

   GFP-fGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-DEST47 PINK1 C-GFP

   Plasmid

   #13316

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICS LY-MT Short P2A

   Plasmid

   #213614

   Purpose

   Detect the short-range Lysosome-Mitochondria contact

   Depositor

   Tito Calì

   Article

   Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

   Insert

   SPLICS LY-MT Short P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

   Plasmid

   #203652

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire-IRES-H2B-mCherry

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. anxA1-GFP

   Plasmid

   #107194

   Purpose

   Expresses porcine annexin A1-GFP fusion protein for live cell imaging

   Depositor

   Ursula Rescher, Volker Gerke

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A1
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV Puro DEST (w118-1)

   Plasmid

   #17452

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Puro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lck-mScarlet-I

   Plasmid

   #98821

   Purpose

   In vivo visualization of the plasma membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   Lck
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-sgRNA puro

    Plasmid

    #104990

    Purpose

    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid

    #203653

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. JD171

    Plasmid

    #229483

    Purpose

    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, VA RNA I, L4-22k/33k
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBP-MS2

    Plasmid

    #65104

    Purpose

    can be used to isolate RNA binding complexes

    Depositor

    Josep Vilardell

    Article

    Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

    Insert

    MS2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    double mutation (V75Q and A81G) that prevents oli…

    Promoter

    tac

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZR071_SFFV-dCas9-mCherry-KRAB

    Plasmid

    #180264

    Purpose

    Lentiviral expression vector for dCas9-KRAB in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    KRAB and mCherry

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4xNFkB Luc

    Plasmid

    #111216

    Purpose

    reporter gene

    Depositor

    Johannes A. Schmid

    Insert

    firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-Hylight

    Plasmid

    #193447

    Purpose

    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

    Depositor

    Richard Goodman

    Article

    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Hylight Biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

    Plasmid

    #167935

    Purpose

    Inducible CRISPRi expression

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    KRAB-dCas9-DHFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    tetON

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-GRAB-rDA3h

    Plasmid

    #208703

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA3h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV(CAT)T7-SB100

    Plasmid

    #34879

    Depositor

    Zsuzsanna Izsvak

    Article

    Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

    Insert

    SB100X transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sec61b-V5-TurboID

    Plasmid

    #166971

    Purpose

    Expresses ER targeted TurboID for secretome biotinylation

    Depositor

    Hyun-Woo Rhee

    Article

    Kim et al Nat Commun. 2021 Sep 1;12(1):5204. doi:

    Insert

    Sec61b-V5-TurboID
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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