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pIRES-hrGFP II-mTET1_CD

Plasmid

#83571

Purpose

Expresses a mutant catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted, catalytic domain…

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Fuw-AcrIIA4-P2A-GFP

Plasmid

#108247

Purpose

Lentiviral vector to express AcrIIA4-P2A-GFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

AcrIIA4-NLS-HA (NEWENTRY Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Ubc

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-mTET1

Plasmid

#83569

Purpose

Expresses TET1 with a mutated catalytic domain in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

catalytic domain mutant H1672D, D1674A

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

Plasmid

#138147

Purpose

Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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His-lsMSP1D1dH5

Plasmid

#240150

Purpose

Expression of the MSP 'His-lsMSP1D1dH5'

Depositor

Lise Arleth

Article

Tidemand et al Biochim Biophys Acta Biomembr. 2022 Jun

Insert

His-lsMSP1D1dH5

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Apple-53BP1trunc

Plasmid

#69531

Purpose

Fluorescent reporter for DNA damage

Depositor

Ralph Weissleder

Article

Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

Apple fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-EGFP-WPRE-pA

Plasmid

#37084

Purpose

Cre-dependent expression of EGFP

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-on

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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Ub-G76V-YFP

Plasmid

#11949

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

Ub-G76V-YFP

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of YFP. Glycine 76 …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pHEN2-SG4

Plasmid

#196071

Purpose

Express wild type SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4

Tags

3XFLAG and 6XHis

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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FUGW-PercevalHR

Plasmid

#49083

Purpose

Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-UbC-rtTA-Ngn2:2A:EGFP

Plasmid

#127288

Purpose

all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

Depositor

Fred Gage

Article

Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

Insert

Ngn2:2A:Ascl1 (NEUROG2 Human)

Use

Lentiviral

Tags

Ngn2 and EGFP linked via 2A

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-N90-beta-catenin

Plasmid

#31785

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

beta-catenin (CTNNB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of aa 1-90- Constitutitvely Active

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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APEX2-NLS

Plasmid

#124617

Purpose

proximity biotinylation

Depositor

Alice Ting

Article

Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

Insert

APEX2-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 flag p53

Plasmid

#10838

Depositor

Thomas Roberts

Article

Gjoerup et al J Virol. 2001 Oct . 75(19):9142-55.

Insert

p53 (TP53 Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pMXs-3XHA-EGFP-OMP25

Plasmid

#83356

Purpose

For tagging mitochondria with HA epitopes (HA-MITO construct)

Depositor

David Sabatini

Article

Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

Insert

3XHA-EGFP-OMP25

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A

Plasmid

#28047

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1

Plasmid

#218796

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1

Expression

Mammalian

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-307_LgBiT

Plasmid

#191211

Purpose

Lentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells

Depositor

Mohan Babu

Article

Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:

Insert

LgBiT (large bit)

Use

Lentiviral and Luciferase

Mutation

AB030246.1 (mutated in PMID 26569370)

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAIDA1

Plasmid

#79180

Purpose

A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

Depositor

Gen Larsson

Article

Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

Insert

Adhesin Involved in Diffuse Adherence

Tags

His and Myc

Expression

Bacterial

Promoter

lacUV5

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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