We narrowed to 8,526 results for: construct
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Plasmid#15327DepositorInsertEleven Nineteen Lysine Rich in Leukemia 2 (ELL2 Human)
UseBaculovirus constructionTagsFLAGAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
Plasmid#159801PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKC123
Plasmid#134240Purposeexpresses gRNA targeting CENP A 3'DepositorInsertCENP A 3' CRISPR construct
UseCRISPRExpressionMammalianAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJL3
Plasmid#184131PurposeFor rescuing null mrp-1 mutation and labelling mrp-1 protein. Intestinal specific Pges-1 promoter drives MRP-1 expression. Translational fusion construct. pPD95.75_Pges-1::mrp-1(isoform C cDNA)::gfp.DepositorAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EAF2 pBacPAK8
Plasmid#15326DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIhh1.3K-Luc
Plasmid#53604PurposeReporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
UASgal-A9-Pleum
Plasmid#60343PurposeContains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceNov. 6, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLG1-puro-sgATL3-2
Plasmid#109010PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BXG-2TAG
Plasmid#158138PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)DepositorInsertBFP-GFP fragment
ExpressionYeastMutationCloned in a TAG codon at the first serine residue…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71139
Plasmid#154062PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)DepositorInsertpZmUbi
UseSynthetic BiologyMutationAll BsaI, Esp3I and BpiI restriction sites were r…Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDJ16 - pEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
Plasmid#159802PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | mMaple3 (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Major Sat-BUB1
Plasmid#133350PurposeExpress a TALE construct that recognizes major satellite repeats fused to mClover and the kinase domain of mouse BUB1 (aa 672-1058) at the C terminusDepositorAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
His10-PS-SNAPf-9E (XSB526)
Plasmid#78514PurposeConstruct for expressing His10 - SNAPf -9E in E. ColiDepositorInsertSNAPf
TagsHis10 followed by a prescission protease claevage…ExpressionBacterialMutation9E added to the C-terminal of SNAPfAvailable SinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_BpiI-cloning-site_SV40polyA
Plasmid#124547PurposeDestination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pconst #3-RFP
Plasmid#81077Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23101Available SinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCV-HF LTS220A/S239A
Plasmid#112191PurposeMerkel cell polyomavirus molecular cloneDepositorInsertMCV-HF LTS220A/S239A
MutationT to G mutations at 3313 (nt) and 3370 (nt) of fu…PromoterNone from vector. Insert contains native promoter…Available SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL3-5
Plasmid#109011PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2
Plasmid#141052PurposeMutisite Gateway mediated vector constructionDepositorInserteSpCas9(1.1)-2A-EGFP
ExpressionMammalianAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pconst #2-RFP
Plasmid#81076Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter- BBa_J23104Available SinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLz74_Rubisco_CsoS2_Npeptide_fusion
Plasmid#140840PurposeSame as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallizationDepositorInsertForm IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
TagsCsoS2 N-peptide consensus to CbbL C-terminus and …ExpressionBacterialPromoterT7Available SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only