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173,847 results

Showing: 9421 - 9450 of 173847 results

  2. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  6. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Article
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    100936-plasmid-map-sequence-id-206124

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  7. pPOTv7-hyg-OsAID (p5110)

    Plasmid
    #239360
    Purpose
    pPOTv7 based plasmid template for addition of a Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Inserts
    Rice Auxin Inducible Degradation domain
    Auxin Inducible Degradation domain
    Use
    Bacterial
    Promoter
    none
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239360-plasmid-map-sequence-id-478808

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  8. pPOTv6-puro-3Ty:OsAID:3Ty (p5229)

    Plasmid
    #239361
    Purpose
    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Insert
    Rice Auxin inducible degradation domain
    Use
    Bacterial
    Tags
    3 x Ty epitope tag
    Promoter
    none
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239361-plasmid-map-sequence-id-478802

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  9. pPOTv6-hyg-3Ty:OsAID:3Ty (p5230)

    Plasmid
    #239362
    Purpose
    pPOTv6 based plasmid template for addition of Ty-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using puromycin
    Depositor
    Mark Carrington
    Article
    Alexandra et al bioRxiv 2025.06.17.660065
    Insert
    Rice Auxin inducible degradation domain
    Use
    Bacterial
    Tags
    3 x Ty epitope tag
    Promoter
    n/a
    Available Since
    July 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    239362-plasmid-map-sequence-id-478759

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  16. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid
    #72639
    Purpose
    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    Use
    Gateway Cloning
    Mutation
    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72639-plasmid-map-sequence-id-429021

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  19. TFORF2302

    Plasmid
    #142018
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CIITA (CIITA Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142018-plasmid-map-sequence-id-395919

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  20. mCherry-MAPTau-C-10

    Plasmid
    #55077
    Purpose
    Localization: Microtubules, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    MAPTau (MAPT Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55077-plasmid-map-sequence-id-353040

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Showing: 9421 - 9450 of 173847 results