159,557 results

1. pBEVY-GU

   Plasmid

   #51229

   Purpose

   bi-directional expression vector for yeast, galactose inducible, URA3 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1,10

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLGB13

   Plasmid

   #126618

   Purpose

   Suicide vector for allelic replacement in Bacteroides, erythromycin selection and aTC-inducible ss-Bfe1 counterselection

   Depositor

   Laurie Comstock

   Article

   Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

   Type

   Empty backbone

   Use

   Allellic exchange in bacteria

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-link-sfGFP-FLAG:Kan

   Plasmid

   #211833

   Purpose

   Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   sfGFP-FLAG-Kan
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

   Plasmid

   #207180

   Purpose

   Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMDJ231 - Phsp Cas9

   Plasmid

   #191382

   Purpose

   Heat shock inducible Cas9 expression in C. elegans.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   Phsp-16.41 Cas9 gpd-2 tagRFP-t
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Phsp-16.41

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 Flag p38 alpha

   Plasmid

   #20351

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pDCNA3 Flag p38 alpha (Mapk14 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

   Plasmid

   #100842

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0096 RESCUE truncation (Cas13 delta 892-1095)

   Plasmid

   #130663

   Purpose

   CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

   Insert

   dRanCas13b(delta 892-1095)-ADAR2dd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-BCL6-IRES-GFP

   Plasmid

   #31391

   Depositor

   Hilda Ye

   Article

   Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

   Insert

   B-cell lymphoma 6 (BCL6 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. SlugCas9

    Plasmid

    #163793

    Purpose

    Expresses SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-mEOS3.2-KanMX6

    Plasmid

    #87030

    Purpose

    Tag S. pombe genes in C terminal with mEOS3.2

    Depositor

    Julien Berro, Thomas Pollard

    Article

    Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    mEOS3.2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHIV-Luciferase

    Plasmid

    #21375

    Purpose

    self-inactivating 3rd generation lentiviral plasmid for co-expression of your gene of interest and Luciferase

    Depositor

    Bryan Welm

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLOX-TERT-iresTK

    Plasmid

    #12245

    Purpose

    2nd generation lentiviral vector for reversible immortalization of human primary cells

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    TERT, HSV1-TK (TERT Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTARA

    Plasmid

    #31491

    Depositor

    Kathleen Matthews

    Article

    Wycuff et al Anal Biochem. 2000 Jan 1. 277(1):67-73.

    Insert

    RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N823D

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB1.HMBP.PrS.PHF1

    Plasmid

    #125167

    Purpose

    Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF1 (PHF1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. B-GRAM-W

    Plasmid

    #211707

    Purpose

    Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Tryptophan

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-His-TEV-NQO1

    Plasmid

    #119058

    Purpose

    For expression in E.coli and affinity purification of N-terminally His tagged NQO1

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    6His, TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)

    Plasmid

    #164457

    Purpose

    AiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. EGFP-Talin head-SpyCatcher003

    Plasmid

    #133566

    Purpose

    Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    EGFP-Talin head-SpyCatcher003
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CaMKIIa-hChR2(H134R)-EYFP

    Plasmid

    #26969

    Purpose

    AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Histidine 134 is mutated to Arginine

    Promoter

    CaMKIIa

    Available since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHMGCS-Luc (aka: pES8)

    Plasmid

    #14947

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    HMG CoA synthase promoter
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCMV-PE5max-SF3B1-K700E

    Plasmid

    #200317

    Purpose

    Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Inserts

    K700E-ng1+pg2
    PEmax-2A-MLH1dn
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. prDNA spacer-LacOx50-20xMS2-Hyg

    Plasmid

    #210219

    Purpose

    Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. SpCas9-NRCH-ABE8e(V106W)

    Plasmid

    #198556

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAAV-Bcl2

    Plasmid

    #195551

    Purpose

    Expresses Bcl2 in mammalian cells

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Bcl2 (Bcl2 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer and promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. Flag-HA-USP10

    Plasmid

    #22543

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP10 (USP10 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GFP-wGBD

    Plasmid

    #26734

    Depositor

    William Bement

    Article

    Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

    Insert

    Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)
    

    Use

    Reporter for cdc42

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Cdc42 binding domain of N-WASP

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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