-
Plasmid#61372Purpose5' Gateway Entry Clone Containing 4xnrUASDepositorInsert4xnrUAS
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87E/S129E
Plasmid#36064DepositorInsertalpha-synuclein S87E/S129E (SNCA Human)
UseTagsExpressionBacterialMutationS87E/S129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A/S129A
Plasmid#36063DepositorInsertalpha-synuclein S87A/S129A (SNCA Human)
UseTagsExpressionBacterialMutationS87A/S129APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S87A/S129E
Plasmid#36065DepositorInsertalpha-synuclein S87A/S129E (SNCA Human)
UseTagsExpressionBacterialMutationS87A/S129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJK141
Plasmid#73316PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p signal sequence, and c-mycExpressionYeastMutationsynthetic genePromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTEF2
Plasmid#43971DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterTEFAvailable sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGPD2
Plasmid#43972DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterGPDAvailable sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT2-5xERE:GFP
Plasmid#125690PurposeTransgene to report estrogen receptor activity in zebrafishDepositorInsert5xERE:GFP
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREDSIX
Plasmid#74103PurposemCherry-encoding vector for marker replacement mutagenesis in Bradyrhizobium diazoefficiens and other AlphaproteobacteriaDepositorTypeEmpty backboneUseConjugative bacterial vectorTagsExpressionMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCYC2
Plasmid#43970DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterCYC1Available sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV asyn S129A
Plasmid#36066DepositorInsertalpha-synuclein S129A (SNCA Human)
UseAAVTagsExpressionMammalianMutationS129APromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S129D
Plasmid#36069DepositorInsertalpha-synuclein S129D (SNCA Human)
UseAAVTagsExpressionMammalianMutationS129DPromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV asyn S129E
Plasmid#36068DepositorInsertalpha-synuclein S129E (SNCA Human)
UseAAVTagsExpressionMammalianMutationS129EPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME-QS (L1-L2)
Plasmid#61378PurposeGateway Middle Entry Vector Containing QSDepositorInsertQS
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK276
Plasmid#73366PurposeEncodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)DepositorInsertplastid omega-3 fatty acid desaturase
UseTagsExpressionYeastMutationMutation I231F. Also insert is missing residues 1…PromoterGAL1Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK142
Plasmid#73317PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p, and c-mycExpressionYeastMutationsynthetic gene. Encodes a glycine-204 to serine m…PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S129G
Plasmid#36067DepositorInsertalpha-synuclein S129G (SNCA Human)
UseAAVTagsExpressionMammalianMutationS129GPromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME-QF
Plasmid#61375PurposeMiddle Gateway Entry Vector Containing QFDepositorInsertQF
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
NK804
Plasmid#166058PurposePuromycin resistance and MRLC-mTFP expression for Salpingoeca rosettaDepositorInsertMRLC-mTFP
UseS. rosetta expressionTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
p5E-QUAS (L4-R1)
Plasmid#61374Purpose5' Gateway Entry Vector Containing QUASDepositorInsertQUAS
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pg-nucODD-EGFP
Plasmid#87212PurposeHIF-1a sequence without the bHLH domain fused to EGFPDepositorInsertHIF-1a nucODD (HIF1A Human)
UseTagsEGFPExpressionMammalianMutationwithout the bHLH domain, Q814RPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S87A/S129A
Plasmid#36070DepositorInsertalpha-synuclein S87A/S129A (SNCA Human)
UseAAVTagsExpressionMammalianMutationS87A/S129APromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S87D/S129A
Plasmid#36071DepositorInsertalpha-synuclein S87D/S129A (SNCA Human)
UseAAVTagsExpressionMammalianMutationS87D/S129APromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GFP
Plasmid#184811PurposeUsed to generate the Tg(QUAS:GFP)c578 transgenic lineDepositorInsertQUAS:GFP-pA
UseZebrafish tol2 transgenesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSZPKIaHAERT(#118)
Plasmid#184075Purposecyclofen-inducible PK inhibitor alpha (wt) activation via mammalian cell transfection or mRNA synthesisDepositorInsertZfPKAinhiba-HA-ERT2
UseSynthetic BiologyTagsinternal tag: HAExpressionMammalianMutationERT2: G400V, M543A, L544A, deleted 1-281;PromotersCMV+SP6Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GFP-CAAX
Plasmid#184813PurposeUsed to generate the Tg(QUAS:GFP-CAAX)c591 transgenic lineDepositorInsertQUAS:GFP-CAAX-pA
UseZebrafish tol2 transgenesisTagsGFP-CAAXExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZE-PalkB-GFP
Plasmid#44381DepositorInsertsGFP
PalkB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPalkBAvailable sinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACYC-PLlambda-alkSm4-3
Plasmid#44383DepositorInsertAlkSm4-3
UseSynthetic BiologyTagsExpressionMutationQ410I, S470T, K546E, E775DPromoterPLlambdaAvailable sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACYC-PLlambda-alkS(WT)
Plasmid#44382DepositorInsertAlkS(WT)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlambdaAvailable sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTETSIX
Plasmid#74104PurposemCherry-encoding vector for markerless deletion mutagenesis in Bradyrhizobium diazoefficiens and other AlphaproteobacteriaDepositorInserttetA(C)
UseConjugative bacterial vectorTagsExpressionMutationC-to-T transition at pos. no. 1049 of tetA(C) lea…PromoterAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPromoterGAL1 and GAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZL1-ZF - myelin basic protein a (mbpa)
Plasmid#103007PurposePlasmid template for making in-situ hybridization probe for myelin basic protein a (mbpa) gene zf-fj330a07.y1.DepositorInsertmyelin basic protein (mbpa Zebrafish)
UseTagsExpressionMutationPromoterSp6Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSZPKIamutHAERT(#119)
Plasmid#184076Purposecyclofen-inducible PK inhibitor alpha (mut) activation via mammalian cell transfection or mRNA synthesisDepositorInsertZfmutPKAinhiba-HA-ERT2
UseSynthetic BiologyTagsinternal tag: HAExpressionMammalianMutationZfPKIa: R19A, R20A; ERT2: G400V, M543A, L544A, de…PromotersCMV+SP6Available sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmRFP_a_tubulin_IRES_puro2b
Plasmid#21041DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagspmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCIG-VP16 RARa [TJ#389]
Plasmid#16287DepositorInsertRAR alpha (RARA Human)
UseTagsVP16ExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
pDestTol2-QUAS:GCaMP7a
Plasmid#184817PurposeUsed to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).DepositorInsertQUAS:GCaMP7a-pA
UseZebrafish tol2 transgenesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIS-krPAM1
Plasmid#25456DepositorInsertPAM1 (Pam Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
pKG204
Plasmid#63078PurposeRetroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)DepositorInsertHP1 alpha (CBX5 Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationV151I mutation compared to GenBank reference sequ…PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-Leum
Plasmid#44364DepositorInsertLeum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterLeum promoterAvailable sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDestTol2-QUAS:mApple-he1.1:CFP
Plasmid#184812PurposeUsed to generate the Tg(QUAS:mApple; he1.1:CFP)c788 transgenic lineDepositorInsertQUAS:mApple-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK522-FAST-PETase
Plasmid#214754Purposeplasmid for expression FAST-PETase in Pseudomonas putidaDepositorInsertFAST-PETase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
psd44-GqQL
Plasmid#46826Purpose2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cellsDepositorInsertg protein alpha q (GNAQ Human)
UseLentiviralTagsExpressionMammalianMutationQ209L (The mutation reduces GTPase activity resul…PromoterPGK promoterAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
ykgMO-IGS-LuxCDABE
Plasmid#207232PurposeCalprotectin sensing promoter with luciferase reporterDepositorInsertykgMO-IGS
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-Leum
Plasmid#44316DepositorInsertUAS1B8-Leum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-LeumAvailable sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmRFP_a_tubulin_C1
Plasmid#21040DepositorInsertalpha Tubulin (TUBA1B Human)
UseTagspmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only