We narrowed to 73,945 results for: LAS

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pMXS-IRES-Blast FLVCR1b

Plasmid

#218535

Purpose

human FLVCR1b cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Alternative isoform

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSL3927 (pDonor(plasmid)_Pse)

Plasmid

#200905

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016,with a CmrR cargo, as well as a primer binding site for deep sequencing purposes at pTarget. Total transposon size = 1,087 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (pTarget NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-mScarlet-BlastR [M1G]

Plasmid

#171814

Purpose

Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

Depositor

Michael Hoelzel

Article

Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

Insert

mScarlet-T2A-BlastR

Use

Gene tagging

Mutation

BlastR: Changed Methionin 1 to Glycine.

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJAM1.16_PB-Cx43_S368A-PGK-Blast

Plasmid

#205561

Purpose

Express human Cx43 with S368A mutation

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Cx43

Use

Piggybac

Expression

Mammalian

Mutation

S368A

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pJAM1.17_PB-STEAP3-PGK-Blast

Plasmid

#205562

Purpose

Express human STEAP3

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

STEAP3 (STEAP3 Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMH-HA/Joseph2-Multitagged Plasmid

Plasmid

#226726

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pMH-HA/Joseph2

Tags

HA, Myc, GFP, Flag, V5, His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Blast-mNeon-ACTB

Plasmid

#207751

Purpose

Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCLIPf-Cox8A Control Plasmid

Plasmid

#101130

Purpose

Expression of CLIP-Tagged Cox8A in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Cox8A (COX8A Human)

Tags

CLIP-tag (CLIPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-CLASP2 512-650

Plasmid

#24409

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) (CLASP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant with only MT plus end trac…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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pCDHblast MCSNard OST-LMNA

Plasmid

#22661

Depositor

Tom Misteli

Article

Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

Onestrep Tag

Expression

Mammalian

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast mAtg7

Plasmid

#173170

Purpose

Retroviral vector to express sgRNA resistant mouse Atg7

Depositor

Kivanc Birsoy

Article

Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available Since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 recording plasmid

Plasmid

#194211

Purpose

CJ8421_04975 Rptr2 targeted DNA sequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 Rptr2 DNA target

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST TFR1

Plasmid

#169890

Purpose

Express TFR1 open reading frame. Lacks IRE regulatory elements

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

TFRC (TFRC Human)

Use

Retroviral

Expression

Mammalian

Mutation

TFRC cDNA, lacking 3'UTR regulatory elements

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast HA-POLE2

Plasmid

#160809

Purpose

Express HA-tagged POLE2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

HA-POLE2 (POLE2 Human)

Use

Retroviral

Tags

HA

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CHKA

Plasmid

#218540

Purpose

human CHKA cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Blast

Plasmid

#175270

Purpose

MegaGate destination with C-terminal HA tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mCherry

Plasmid

#169411

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mCherry

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-AIDmax-BlastR

Plasmid

#152994

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

AID-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCLIPf-H2B Control Plasmid

Plasmid

#101134

Purpose

Expression of CLIP-tagged Histone H2B in mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Histone H2B (H2BC11 Human)

Tags

CLIP-tag (CLIPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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