We narrowed to 13,703 results for: cas9

1. pV132-vCas9

   Plasmid

   #203223

   Purpose

   Mammalian expression of vCas9

   Depositor

   Vikash Chauhan, Robert Langer, Phil Sharp

   Article

   Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   vCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   S55R R976A K1003A T1314R

   Promoter

   CBh

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR4-Cas9-V5

   Plasmid

   #87905

   Purpose

   Cloning vector

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   Cas9-V5
   

   Use

   Cloning vector

   Tags

   V5

   Expression

   Mutation

   Human codon optimized

   Promoter

   V5

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPbU6_hdhfr/yfcu_Cas9

   Plasmid

   #216423

   Purpose

   Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter and the Cas9 nuclease for traditional CRISPR editing.

   Depositor

   Ellen Bushell

   Article

   Jonsdottir et al bioRxiv 2024.04.20.590404

   Type

   Empty backbone

   Use

   CRISPR; Expression in plasmodium berghei

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PRE-Hsp70BbCas9_1.2

   Plasmid

   #190796

   Purpose

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-T3-eSpCAS9-NG-pA

   Plasmid

   #131466

   Purpose

   Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized eSpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-DNMT3L

   Plasmid

   #126588

   Purpose

   DNMT3L fused to C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

   Insert

   DNMT3L (DNMT3L Human)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-dCas9-DNMT3L-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. ACE2 g4 + Cas9

   Plasmid

   #153014

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9-VRER_2A_GFP

   Plasmid

   #75475

   Purpose

   Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

   Depositor

   Marc Tessier-Lavigne

   Article

   Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

   Insert

   Cas9-VRER
   

   Use

   CRISPR

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   CAG

   Available since

   May 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGM4723:Ble:Cas9

   Plasmid

   #112208

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.

   Depositor

   Thomas Mock

   Inserts

   Cas9
   SheBle
   

   Use

   CRISPR; Diatom, phaeodactylum

   Tags

   Phaeodactylum fcp promoter and terminator and YFP

   Expression

   Mutation

   Phaeodactylum Fcp promoter

   Promoter

   Phaeodactylum Fcp

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. SaCas9 TadCBEa

    Plasmid

    #193844

    Purpose

    Express TadCBEa (with SaCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    SaCas9 TadCBEa
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX165-HypaCas9

    Plasmid

    #140567

    Purpose

    Expresses HypaCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    N692A M694A Q695A H698A

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL7_2xNLS_iGeoCas9(G)_2xNLS_His

    Plasmid

    #222989

    Purpose

    Bacterial expression of iGeoCas9(G) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(G)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGB-SpCas9

    Plasmid

    #210533

    Purpose

    Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    SpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMCSG7-dNmeCas9

    Plasmid

    #71475

    Purpose

    bacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-Inactive-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    D16A H588A

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9 iFE

    Plasmid

    #190138

    Purpose

    Expresses SpCas9-POLB (iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9-POLB (HiFi iFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF(UG) hSyn spCas9

    Plasmid

    #179725

    Purpose

    human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

    Plasmid

    #110300

    Purpose

    Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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