We narrowed to 13,937 results for: cas9

1. pMonAID_nCas9-PmCDA_Hyg_ALS

   Plasmid

   #91693

   Purpose

   Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   2x35S

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACT2-CAS9c

   Plasmid

   #51055

   Purpose

   Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   Cas9c
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial and Yeast

   Promoter

   yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. BB1_23_dCAS9(3xFLAG_SV40NLS)

   Plasmid

   #161478

   Purpose

   BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Insert

   dCAS9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   FLAG Tag, SV40 NLS

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

   Plasmid

   #167898

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A_dCas9-1x2

   Plasmid

   #63596

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D1361Y

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. P628_SpCas9-RecA

   Plasmid

   #87263

   Purpose

   Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   no

   Promoter

   CBh

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ-dpcoCas9-TV

   Plasmid

   #158409

   Purpose

   CRISPR-dpcoCas9 fused with TV activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-TV(6xTAL-2xVP64)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZ8-P_dCas9

   Plasmid

   #74063

   Purpose

   pZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   dcas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Propionate inducible promoter (prp)

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH41308::hCas9

   Plasmid

   #49770

   Purpose

   Level 0 hCas9 module

   Depositor

   Sophien Kamoun

   Article

   Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

   Insert

   hCas9
   

   Use

   CRISPR

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. vas-Cas9.874Z

    Plasmid

    #112687

    Purpose

    Express hSpCas9 under vasa promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    vasa

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbdCas9S_P0526-sgRNAmreB

    Plasmid

    #149654

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMA-SpCas9-g7

    Plasmid

    #80790

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. eSpCas9-EF-5Nanog_2

    Plasmid

    #174871

    Purpose

    CRISPR vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p(bhsp68-Cas9)

    Plasmid

    #65959

    Purpose

    Tribolium basal heat shock promoter driving Cas9

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    nuclear localization sequence

    Expression

    Insect

    Promoter

    bhsp

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiRCas9-Lambda2

    Plasmid

    #104184

    Purpose

    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)

    Depositor

    Eugene Yeo

    Article

    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do

    Insert

    U6-sgRNA, EFS-PIN-dCas9
    

    Use

    CRISPR and Lentiviral

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9 TadCBEc

    Plasmid

    #193834

    Purpose

    Express TadCBEc (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDc-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-dCas9_puroR

    Plasmid

    #219825

    Purpose

    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    CMV

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. UAS-u(S)Cas9

    Plasmid

    #127383

    Purpose

    Expresses Cas9 at high level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(S)-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    UAST

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSMVP-Cas9N

    Plasmid

    #80934

    Purpose

    Plasmid that expresses Cas9N in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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