159,589 results
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Plasmid#122651PurposeExpression of P22 virus like particle in bacteriaDepositorInsertP22 Coat: WT / SP: 6xHis-SP141
UseTagsExpressionBacterialMutationtruncated SP, P120H in the capsid scaffold (see …PromoterAvailable sinceAug. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7s
Plasmid#104463PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7s
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3232
Plasmid#144708PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertJUNB (JUNB Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF463
Plasmid#167671PurposeIntermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-Hyg-Dest-mCherry-PAR-6B
Plasmid#194908PurposeTo generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.DepositorInsertPAR-6B (Pard6b Mouse)
UseMouse TargetingTagsmCherryExpressionMammalianMutation39 C to G, silent mutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.PHF1
Plasmid#125167PurposeExpresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertPHF1 (PHF1 Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB533_2E12LI-sfGFP
Plasmid#167527PurposeH3K27me3-mintbody expression vector for mammalian cellsDepositorInsert2E12LI-scFv
UseTagssfGFPExpressionMammalianMutationPromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-PD-1-SNAP
Plasmid#223576PurposeLentiviral expression of human PD-1 with SNAP tag in mammalian cellsDepositorInsertPD-1 (PDCD1 Human)
UseLentiviral; mammalian expressionTagsSNAPExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)
Plasmid#164457PurposeAiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neuronsInsertSYFP2
UseAAVTagsExpressionMutationPromoterminCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES2-HTH-HHT2
Plasmid#86482Purposeyeast expression of HHT2 with His10-TEV-HA tagDepositorInsertHHT2 (HHT2 Budding Yeast)
UseTagsHIS10-TEV-HA (HTH)ExpressionYeastMutationPromoterGAL1Available sinceJan. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHLmMBP-10
Plasmid#72348PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FLAG-METTL3
Plasmid#160250PurposeExpress FLAG-METTL3 in human cellsDepositorInsertmethyltransferase like 3 (METTL3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40
Plasmid#216533PurposeAn axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switchDepositorInsertaxon-jGCaMP8m
UseAAV and Cre/LoxTagsGAP43ExpressionMammalianMutationPromoterhSynapsin1Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-TRE-AFOS
Plasmid#197079PurposeA doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.DepositorInsertAFOS (Fos Mouse)
UseLentiviralTagsFlag and T7ExpressionMutationPromoterTREAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Cs
Plasmid#183171PurposeConstruct for transient expression of cytoskeleton-targeted mCherry in plants.DepositorInsertmCherry-Cs
UseTags363 aa of A. thaliana FIMBRIN 1 (AtFIM1)ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-10Myc
Plasmid#181939PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xMyc
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOG1336
Plasmid#167303PurposeLevel 0 module (3U+Ter) for further assemblies based on the Modular Cloning system. 3'UTR and flanking sequence of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsert3'UTR and Terminator SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOG1334
Plasmid#167302PurposeLevel 0 module (Pro+5U) for further assemblies based on the Modular Cloning system. Promoter and 5'UTR of a Solanum lycopersicum Oleosin gene, arbitrarily called OLE2 (Sollyc03g112440)DepositorInsertPromoter SlOLE2
UseSynthetic Biology; Moclo level 0 vectorTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
ES-FUCCI
Plasmid#62451PurposeSMX_DEST vector with 3 cassettesDepositorInsertsminPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-IL8
Plasmid#189608PurposeRetroviral expression vector for IL8DepositorInsertIL8 (CXCL8 Human)
UseRetroviralTagsExpressionMutationPromoterSV40 early promoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/HF-CDC50A
Plasmid#209223PurposeMammalian expression of CDC50ADepositorInsertCDC50A (TMEM30A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBA439
Plasmid#85967PurposePerturb-seq vector backboneDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1 hygro mCherry
Plasmid#128057PurposeMammalian expression vector with N terminal mCherryDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-IRES-Gβγ-BERKY3
Plasmid#158426PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-HygDepositorInsertLyn11-Nluc-ER/K linker-3xYFP-GRK3ct
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE
Plasmid#161926PurposeLentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAGDepositorInsertrTetR(SE-G72P)
UseLentiviral and Synthetic BiologyTags3XFLAGExpressionMammalianMutationPromoterpEFAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUSE2ss-CLIg-mK_M18
Plasmid#82358PurposeExpression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertUseTagsnoneExpressionMammalianMutationPromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-3xFLAG
Plasmid#207684PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA6-N-Myc-SCAP
Plasmid#134285PurposeExpresses Myc-tagged SCAP in mammalian cellsDepositorUseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only