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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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178,737 results

Showing: 9601 - 9630 of 178737 results

  2. pAD-GamS

    Plasmid
    #99246
    Purpose
    Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.
    Depositor
    Jeff Hasty
    Publication
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    gamS (with C-terminal 6xHis tag)
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T7lac
    Available since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99246-plasmid-map-sequence-id-339990

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  3. pcDNA3-teLuc c-myc

    Plasmid
    #100026
    Purpose
    Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)
    Depositor
    Huiwang Ai
    Publication
    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    teLuc, a NanoLuc mutant
    Tags
    c-myc
    Expression
    Mammalian
    Mutation
    D19S, D85N, C164H
    Promoter
    CMV
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100026-plasmid-map-sequence-id-194894

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  9. pEN395 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE targeting

    Plasmid
    #92141
    Purpose
    Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection. Auxin-inducible degron system.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    osTir1
    Use
    Mouse Targeting
    Tags
    V5 tag
    Expression
    Mammalian
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92141-plasmid-map-sequence-id-177217

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  10. mApple-Sec61b-C1

    Plasmid
    #90993
    Purpose
    Expresses mApple-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells
    Depositor
    Jennifer Lippincott-Schwartz
    Publication
    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mApple-Sec61b (SEC61B Synthetic, Human)
    Tags
    mApple (red fluorescence)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90993-plasmid-map-sequence-id-180319

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  13. IL2Rα-EGFP

    Plasmid
    #86055
    Purpose
    A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Interleukin-2 receptor subunit alpha (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86055-plasmid-map-sequence-id-177921

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  16. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87829
    Purpose
    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Publication
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    MTS
    NLS
    PTS1
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87829-plasmid-map-sequence-id-175007

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  21. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  24. CAG sHRPa-NRX

    Plasmid
    #73143
    Purpose
    Large fragment of split HRP fused to the extracellular terminus of neurexin3B. CAG promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPa-NRX
    Tags
    2x HA tag and Flag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    73143-plasmid-map-sequence-id-134511

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Showing: 9601 - 9630 of 178737 results