59,541 results

1. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

   Plasmid

   #177435

   Purpose

   To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.

   Depositor

   David Andrews

   Insert

   Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. eTC GFP beta-actin full length

   Plasmid

   #27123

   Depositor

   Robert Singer

   Article

   Rodriguez et al J Cell Biol. 2006 Oct 9. 175(1):67-76.

   Insert

   beta-actin (ACTB Human)
   

   Tags

   EGFP and TC tag

   Expression

   Mammalian

   Mutation

   E237A

   Available Since

   Jan. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A)

   Plasmid

   #84777

   Purpose

   Second generation lenti expressing Mash1 and Neurogenin for iN transdifferentation to neurons

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Insert

   Ngn2 and Mash1 with a T2A (NEUROG2, ASCL1 Human)
   

   Use

   Lentiviral

   Promoter

   pTIGHT

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG.CASP3-Myc

   Plasmid

   #200119

   Purpose

   Expresses C-terminal Myc-tagged caspase 3 in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   caspase 3 (CASP3 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EIF2AK3 gRNA (BRDN0001146328)

   Plasmid

   #77167

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK3

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK3 (EIF2AK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAC150-RFP-EGFP-LGALS3

   Plasmid

   #175778

   Purpose

   PiggyBac Vector expressing GFP-RFP-LGALS3

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MECP2 in pENTR

   Plasmid

   #216592

   Purpose

   gateway cloning donor vector containing MECP2

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   MECP2 (MECP2 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eIF2a 3

   Plasmid

   #21809

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Expression

   Mammalian

   Mutation

   S52D

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2xFLAGhYAP1-S127A

   Plasmid

   #17790

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   Yes-kinase associated protein mutant S127A (YAP1 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Serine 127 to Alanine

   Available Since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX neo PFKP-GFP

    Plasmid

    #116940

    Purpose

    Expression of PFKP-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    PFKP (PFKP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF1684

    Plasmid

    #143965

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p306-BrdU-Inc

    Plasmid

    #71792

    Purpose

    BrdU-incorporating plasmid with selectable marker URA3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Expression

    Yeast

    Promoter

    ADH1 and GPD

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-R-Rac1

    Plasmid

    #180576

    Purpose

    ddRFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddRFP sensor of Rac1
    

    Expression

    Mammalian

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLENTI_RPS10

    Plasmid

    #127129

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Mutation

    K24R- please see depositor comments

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pH2B-miRFP703

    Plasmid

    #80001

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Tags

    miRFP703

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid

    #112285

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GUT BuGZ FL

    Plasmid

    #84024

    Purpose

    Expression of GFP tagged BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Q394R

    Promoter

    UCOE / SFFV

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-G-Rac1

    Plasmid

    #180575

    Purpose

    ddGFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddGFP sensor of Rac1
    

    Expression

    Mammalian

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NeonGreen-C1-ORP6

    Plasmid

    #220072

    Purpose

    overexpresses ORP6 with a neonGreen fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

    Insert

    OSBPL6 (OSBPL6 Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP Cep170 (Nigg PID200)

    Plasmid

    #41150

    Depositor

    Erich Nigg

    Article

    Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

    Insert

    Cep170 (CEP170 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge