We narrowed to 24,135 results for: CRISPR

Showing: 9691 - 9720 of 24135 results

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LentiV_Cas9_Blast

Plasmid

#125592

Purpose

Lentiviral expression of spCas9 with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

Plasmid

#132404

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

Plasmid

#149722

Purpose

Lentiviral expression of crRNA with GFP and a neomycin resistance gene

Depositor

Junwei Shi

Article

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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PB-UniSAM

Plasmid

#99866

Purpose

Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

Depositor

Lesley Forrester

Article

Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

Insert

UniSAM-mCherry + U6-gRNA2.0

Use

CRISPR and Synthetic Biology; Dcas9-sam activation

Expression

Mammalian

Mutation

BbsI sites in CDS were ablated by consensus mutat…

Promoter

EF1a

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Pepper-fused sgRNA

Plasmid

#217520

Purpose

Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

Depositor

Xing Li

Article

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

Pepper-fused sgRNA for spCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

Plasmid

#101209

Purpose

Bacterial expression plasmid for SpCas9-HF1 variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant N497A/R661A/Q695A/Q926A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

N497A, R661A, Q695A and Q926A

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c1

Plasmid

#120872

Purpose

Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGMC00029 (aka pRC0610)

Plasmid

#200834

Purpose

Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG dCas9-KRAB-2A-EGFP

Plasmid

#92396

Purpose

CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

dCas9_KRAB_2A_EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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CROP-sgRNA-MS2

Plasmid

#153457

Purpose

CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

Depositor

Wolf Reik

Article

Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

Insert

sgRNA empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pdCRISPomyces-2BD

Plasmid

#234429

Purpose

Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

Depositor

Min-Kyu Oh

Article

Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

Insert

Modified dCas9(D10A, H840A) with polyaspartate linking

Expression

Bacterial

Mutation

Linked polyaspartate using glycine-serine linker

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA

Plasmid

#109317

Purpose

Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

Plasmid

#132407

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLwaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R474A; H479A; R1046A; R1051A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_206

Plasmid

#96920

Purpose

for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Cas9-sgRNA-A

Plasmid

#149369

Purpose

Expresses SpCas9 and sgRNA targeting the lenti-CDDR reporter

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Inserts

Cas9
sgRNA-A
Puromycin resistance

Use

CRISPR

Tags

3xFLAG, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CBh, PGK, and U6

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

Plasmid

#132403

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Plasmid

#222994

Purpose

Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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gh50

Plasmid

#106723

Purpose

expression of gRNA targeting ST3GAL2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST3GAL2 (ST3GAL2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEL848

Plasmid

#214238

Purpose

FrCas9 cytosine base editing plasmid V2.1 in rice

Depositor

Yong Zhang

Article

He et al Plant Biotechnol J. 2024 Sep;22(9):2488-

Type

Empty backbone

Expression

Plant

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS

Plasmid

#244842

Purpose

Bacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-Cas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a7

Plasmid

#124846

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pST1374-NLS-flag-linker-Cas9

Plasmid

#44758

Depositor

Xingxu Huang

Article

Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

Insert

Human codon optimized Cas9

Use

CRISPR

Tags

32 amino acid linker, Flag, and NLS

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Perturb-FISH

Plasmid

#220626

Purpose

expression of gRNAs under U6T7 promoter

Depositor

Samouil Farhi

Article

Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

BsmBI

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-ABI-dCas9-P2A-mcherry

Plasmid

#121513

Purpose

expression of AB1-fused-dcas9 plasmids

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

dCas9

Use

Lentiviral

Tags

AB1 and P2A-mcherry

Expression

Mammalian

Promoter

PGK

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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