We narrowed to 1,466 results for: Mbp

1. UR1Ct (265-1040)

   Plasmid

   #115647

   Purpose

   expression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)

   Depositor

   Martin Gellert

   Article

   Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

   Insert

   Rag1 (265-1040) (Rag1 Mouse)
   

   Use

   Tags

   8XHis-MBP-PreScission

   Expression

   Mammalian

   Mutation

   truncated Rag1 (aa 265-1040)

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SLIRP.HMG6.wt.fl

   Plasmid

   #49821

   Purpose

   expression vector for MBP-SLIRP fusion

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   SLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
   

   Use

   Tags

   E. coli Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   wildtype codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAB043a

   Plasmid

   #79222

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ lambda cI-MBP; PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ; PlacIq

   Available since

   April 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJCC_164 SpCas9 R1333A/R1335A

   Plasmid

   #179528

   Purpose

   For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 R1333A/R1335A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginines 1333 and 1335 to alanine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJCC_103 SpCas9 T1337C

   Plasmid

   #179525

   Purpose

   For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 T1337C
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed threonine 1337 to cysteine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. bdSUMO-5.3-GFP-HiBit

   Plasmid

   #171014

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-5.3-muGFP-HiBiT
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EcNusGKOW

   Plasmid

   #113124

   Purpose

   Expresses C-terminus of E. coli NusG

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   NusG (nusG Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-124

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EcRfaHKOW

   Plasmid

   #113125

   Purpose

   Expresses C-terminus of E. coli RfaH

   Depositor

   James Berger

   Article

   Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

   Insert

   RfaH (rfaH Escherichia coli)
   

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Bacterial

   Mutation

   Deleted residues 1-107

   Promoter

   T7

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJB03

    Plasmid

    #111961

    Purpose

    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Inserts

    mEGFP
    eDHFR
    

    Use

    Tags

    DHFR

    Expression

    Bacterial

    Mutation

    eDHFR;residues 2-159 and enhanced GFP with monome…

    Promoter

    Tac

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJ4M_hnRNPA2_FL_WT

    Plasmid

    #139109

    Purpose

    Express FL hnRNPA2 with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMTTH+H1.4

    Plasmid

    #157796

    Purpose

    Expression of human linker histone H1.4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4 (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hCD12

    Plasmid

    #225541

    Purpose

    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid

    #101215

    Purpose

    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K848A/K1003A/R1060A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K848A, K1003A and R1060A

    Promoter

    T7

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJ4M_hnRNPA2_FL_D290V

    Plasmid

    #139110

    Purpose

    Express FL hnRNPA2 D290V with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D290V

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B1 Integrin-GFP

    Plasmid

    #186138

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain with C-terminal GFP tag in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    MBP and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

    Plasmid

    #101205

    Purpose

    Bacterial expression plasmid for SpCas9 REC3 domain

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K506–Q712
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K506–Q712

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJ4M_hnRNPA2_FL_P298L

    Plasmid

    #139111

    Purpose

    Express FL hnRNPA2 P298L with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    P298L

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

    Plasmid

    #101213

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K855A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K855A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. polySUMO1

    Plasmid

    #221066

    Purpose

    Expression of polySUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    Five repeats of SUMO1 each separated by (GGS)4 (SUMO1 Human)
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMAL-c5e-Rubicon RH N821K, T911R, K916R

    Plasmid

    #216707

    Purpose

    Bacterial protein expression of Rubicon RH domain mutant

    Depositor

    James Hurley

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    N821K, T911R, K916R

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PTE_25

    Plasmid

    #111638

    Purpose

    Funclib design PTE_25

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_25
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PTE_26

    Plasmid

    #111639

    Purpose

    Funclib design PTE_26

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_26
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PTE_27

    Plasmid

    #111640

    Purpose

    Funclib design PTE_27

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_27
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PTE_28

    Plasmid

    #111641

    Purpose

    Funclib design PTE_28

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_28
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PTE_36

    Plasmid

    #111642

    Purpose

    Funclib design PTE_36

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_36
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PTE_27.14

    Plasmid

    #111643

    Purpose

    Funclib design PTE_27.14

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_27.14
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. dPTE2

    Plasmid

    #111634

    Purpose

    PROSS stabilized PTE S5

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    dPTE2
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PTE_5

    Plasmid

    #111635

    Purpose

    Funclib design PTE_5

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_5
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PTE_13

    Plasmid

    #111636

    Purpose

    Funclib design PTE_13

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_13
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PTE_24

    Plasmid

    #111637

    Purpose

    Funclib design PTE_24

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

    Insert

    PTE_24
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTac

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant

    Plasmid

    #101206

    Purpose

    Bacterial expression plasmid for SpCas9 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid

    #101218

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A and H698A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET19-MTdTwt

    Plasmid

    #207460

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMTTH+mEGFP-H1.4

    Plasmid

    #157799

    Purpose

    Expression of mEGFP and human linker histone H1.4 fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-H1.4 (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMTTH+H1.4ΔCTD

    Plasmid

    #157797

    Purpose

    Expression of human linker histone H1.4 without c-terminal tail

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H1.4ΔCTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

    Plasmid

    #101202

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    M1–N497, GGS, V713–D1368

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid

    #101233

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G582A, V583A, E584A, D585A and N588A

    Promoter

    T7

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge