We narrowed to 3,402 results for: aaas

Showing: 1321 - 1360 of 3402 results

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pSIN-Arkadia-gRNA6

Plasmid

#180367

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLeGO.sgGata1.4.RUNX1A.iG2

Plasmid

#181976

Purpose

gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

Depositor

Jan-Henning Klusmann

Inserts

GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
RUNX1A (RUNX1 Human)

Use

Lentiviral

Tags

HA

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJM162

Plasmid

#134171

Purpose

Retroviral plasmid expressing GFP Nde1

Depositor

Iain Cheeseman

Article

Monda et al Mol Biol Cell. 2018 Sep 15;29(19):2336-2

Insert

Nde1_SSSC 284-C 318AAA320 (NDE1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

SSSC 284-C 318AAA320

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTBL322 GC

Plasmid

#126950

Purpose

To target a protospacer with GC at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with GC at cut site

Expression

Mammalian

Promoter

human U6

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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gh96

Plasmid

#106756

Purpose

expression of gRNA targeting POFUT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

POFUT1 (POFUT1 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1273

Plasmid

#111112

Purpose

Expresses RAD51B_-_68286555.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

RAD51B gRNA (RAD51B Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-SAC1C389S-HLx8

Plasmid

#108140

Purpose

Inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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AP324-4

Plasmid

#66094

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-3

Plasmid

#66093

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
AP324-2

Plasmid

#66092

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs6

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
lentiGuide-SYNJ2BP-2

Plasmid

#92160

Purpose

CRISPR guide RNA targeting human SYNJ2BP

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP sgRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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pEGFP-Anillin

Plasmid

#68027

Purpose

Full length Anillin rendered RNAi-resistant) in mammalian expression vector pEGFP-C1

Depositor

Michael Glotzer

Article

Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

Insert

Anillin (ANLN Human)

Tags

eGFP

Expression

Mammalian

Mutation

silent mutations at 798 5'-TGCC TCTTTGAATAAA…

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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shPBRM1#32-Tet-pLKO-puro

Plasmid

#107399

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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shPBRM1#131-Tet-pLKO-puro

Plasmid

#107400

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE unc13a GFP KI

Plasmid

#131498

Purpose

Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Unc13a Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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LVDP-CArG-RE-GPR

Plasmid

#89762

Purpose

Quantitative measurement of CArG response element activity.

Depositor

Stelios Andreadis

Article

Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

Insert

6X CArG-RE

Use

Lentiviral

Tags

CMV-ZsGreen

Expression

Mammalian

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shIAPEz_2

Plasmid

#185017

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_IAPEz

Use

Lentiviral

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-GFP

Plasmid

#39291

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Mutation

-1163 to +6 of nmt1 locus with a 7bp deletion (AT…

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pX330 Human 3' HP1a gRNA

Plasmid

#127907

Purpose

WT Cas9 Vector targeting the 3' end of the human HP1a gene

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

gRNA for Human 3' HP1a

Use

CRISPR

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTXB1-TP3

Plasmid

#160086

Purpose

Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase/APEX2 peroxidase fusion protein

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH2_3

Plasmid

#36389

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH2 (EZH2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
mCherry-FKBP-SAC1-HLx2

Plasmid

#108133

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG::SACM1L(1-520):[EAAAR]2:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]2:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_AHR_1

Plasmid

#101076

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1-HLx8

Plasmid

#108136

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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CHEK1 C1.2 gRNA

Plasmid

#90626

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHEK1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CHEK1 (Guide Designation C1.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shFXN_2

Plasmid

#102971

Purpose

Suppression of FXN

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shFXN_2 (FXN Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

Plasmid

#32737

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pENTR/U6 HDAC4 shRNA

Plasmid

#32220

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against murine HDAC4 (Hdac4 Mouse)

Use

RNAi; Gateway u6 entry vector

Promoter

human U6

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
mCherry-FKBP-SAC1-HLx10

Plasmid

#108137

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]10:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]10:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
SMC3 A7.4 gRNA

Plasmid

#90896

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation A7.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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SMC3 H7.1 gRNA

Plasmid

#90894

Purpose

3rd generation lentiviral gRNA plasmid targeting human SMC3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SMC3 (Guide Designation H7.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

Plasmid

#189955

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_ARID5B_1

Plasmid

#86350

Purpose

Encodes gRNA for 3' target of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ARID5B (ARID5B Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECB sgMTHFD2L_2

Plasmid

#106307

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_CEBPG_1

Plasmid

#86344

Purpose

Encodes gRNA for 3' target of human CEBPG

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against CEBPG (CEBPG Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-PRMT9 L182A/D183A/I184A/G185A

Plasmid

#79676

Purpose

mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L182A/D183A/I184A/G185A catalytic mutant

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170817

Purpose

Lentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Insert

NLS-mNeonGreen-P2A-BlastR

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FKBP-SAC1C389S-HLx8

Plasmid

#108138

Purpose

Recruitable inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Topo U6-SpAi9R-U6-SpDMDR

Plasmid

#78609

Purpose

Expresses gRNAs (for SpCas9) targeting 3’ of Ai9 stop cassette and Dmd intron 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SpAi9R-U6-SpDMDR

Use

Unspecified

Promoter

U6

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only
PX458_CEBPG_2

Plasmid

#86345

Purpose

Encodes gRNA for 3' target of human CEBPG

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against CEBPG (CEBPG Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1321 - 1360 of 3402 results

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We narrowed to 3,402 results for: aaas