We narrowed to 23,275 results for: CRISPR

1. qTAG-C-moxGFP-Blast

   Plasmid

   #207710

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pOC1 Halo-Actb KI

   Plasmid

   #183426

   Purpose

   CreON knock-in for Halo-beta-actin (amino acid position: before start codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo donor
   

   Use

   CRISPR

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. dSp-VP64-p65-RTA(75-190)

   Plasmid

   #99670

   Purpose

   Expresses dSp Cas9 fused to combination of truncated activation domains

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Tags

   VP64-p65-RTA(75-190)

   Expression

   Mammalian

   Mutation

   dead Cas9

   Available Since

   May 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRL1

   Plasmid

   #153997

   Purpose

   sgRNA in vitro transcription plasmid.

   Depositor

   Shengdar Tsai

   Article

   Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

   Type

   Empty backbone

   Use

   CRISPR; Sgrna under a t7 promoter for in vitro tr…

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKIKan_idCas9_Chr_center

   Plasmid

   #149637

   Purpose

   suicide vector for knock in into the B. burgdorferi chromosome

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pM198

   Plasmid

   #173218

   Purpose

   Expresses OptGFP(1-9)-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   OptGFP(1-9)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pN-PITCh-HBTH

   Plasmid

   #127883

   Purpose

   Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH tagging

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Insert

   GFP-Puro-HBTH
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pShoCAST-sgRNA_entry (BO1)

   Plasmid

   #181786

   Purpose

   Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
   

   Expression

   Bacterial

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-3xHA-Zeo

   Plasmid

   #207740

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. qTAG-N-Zeo-3xHA

    Plasmid

    #207707

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pTrans_9xD3B_Cluc_Norm

    Plasmid

    #68415

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ172

    Plasmid

    #98409

    Purpose

    Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete lys9 in genome by recombination
    downstream homologous region to delete lys9 in genome by recombination
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZ143-pcDNA3-BvCas12b

    Plasmid

    #122445

    Purpose

    Expresses BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BvCas12b-NLS-3xHA
    

    Tags

    3XHA

    Expression

    Mammalian

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-PpU6P-sgRNA-L1L2

    Plasmid

    #113735

    Purpose

    Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-PpU6P-sgRNA-L5L2

    Plasmid

    #113738

    Purpose

    Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9v2

    Plasmid

    #103140

    Purpose

    TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    dCas9-VPR
    

    Expression

    Yeast

    Mutation

    Removed scaffold gRNA from original plasmid

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330A_D10A-1x7

    Plasmid

    #58777

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-PpU6P-sgRNA-R4R3

    Plasmid

    #113739

    Purpose

    Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-PpU6P-sgRNA-L5L4

    Plasmid

    #113741

    Purpose

    Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-PpU6P-sgRNA-L1R5

    Plasmid

    #113737

    Purpose

    Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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