160,922 results

1. deltaNp63alpha-FLAG

   Plasmid

   #26979

   Depositor

   David Sidransky

   Article

   Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

   Insert

   ΔNP63alpha (TP63 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   dominant negative isoform of p63; aa 1–587

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. Neuroligin-1-GFP/pCMV

   Plasmid

   #197340

   Purpose

   Plasmid expressing an antigen targeting Neuroligin-1

   Depositor

   Thomas Südhof

   Insert

   Neuroligin1 (Nlgn1 Mouse)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gamillus/ pcDNA3

   Plasmid

   #124837

   Purpose

   mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

   Depositor

   Takeharu Nagai

   Article

   Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

   Insert

   Gamillus
   

   Use

   Tags

   No tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_ATF4_WT

   Plasmid

   #82190

   Purpose

   Gateway Donor vector containing ATF4 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF3399

   Plasmid

   #144875

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   POU2F2 (POU2F2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-CRY2-5ptaseOCRL

   Plasmid

   #66836

   Purpose

   Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   5ptaseOCRL (OCRL Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC35F1

   Plasmid

   #132133

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC35F1 (SLC35F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. R701-X71-527: His6-tev-Hs.CALM1(1-80)

   Plasmid

   #159694

   Purpose

   E. coli protein expression of His6-tev-Hs.CALM1(1-80)

   Depositor

   Dominic Esposito

   Article

   Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

   Insert

   His6-tev-Hs.CALM1(1-80) (CALM1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-CRY2oligo-mcherry-ANXA11

   Plasmid

   #164203

   Purpose

   Expresses CRY2oligo-mcherry tagged ANXA11 under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Tags

   CRY2oligo-mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid

    #99690

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRIPZ-bioGEF-UBwt-PURO

    Plasmid

    #208045

    Purpose

    Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    Ub (UBC Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rtn2b-mCherry

    Plasmid

    #186600

    Purpose

    Encodes human full-length Reticulon 2 isoform B fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Human Reticulon 2 isoform B (RTN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

    Plasmid

    #213481

    Purpose

    AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    GFP-KASH
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC1BA

    Plasmid

    #31368

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 May 27. ():.

    Insert

    Cry1 (Cry1 Mouse)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3.1-Nanog

    Plasmid

    #28221

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Jan 18. ():.

    Insert

    Nanog (NANOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p-dCas9-HDAC1-H141A-Hygro

    Plasmid

    #104417

    Purpose

    transient expression of dCas9-dHDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dHDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-Tet-On shYAP1-9

    Plasmid

    #193668

    Purpose

    Tet inducible knockdown of YAP1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    TaU3

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Frb-EGFP-HOTag6

    Plasmid

    #106919

    Purpose

    Express Frb-EGFP-HOTag6 fusion in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    Frb-EGFP-HOTag6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pgex-cIAP1 mut

    Plasmid

    #8334

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    ciap1mut (BIRC2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H588A

    Promoter

    Available since

    Nov. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

21. pET30a-HS-Tn5

    Plasmid

    #127916

    Purpose

    Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Hyperstable Tn5
    

    Use

    Tags

    E coli elongation factor Ts and Mxe intein - Chit…

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcNDA-NES-PhoCl2c-mCherry

    Plasmid

    #164037

    Purpose

    Optogenetic control of protein localization by PhoCl2c

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NES-PhoCl2c-mCherry
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMU-PERr

    Plasmid

    #61202

    Purpose

    Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    mCherry-SKL
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pH2B_mCherry_IRES_puro2

    Plasmid

    #21045

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pH2B (H2BC16P Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

    Plasmid

    #132405

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBS-TRE-tdKatushka2-WPRE

    Plasmid

    #193337

    Purpose

    Expresses tdKatushka2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    tdKatushka2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSLIK DN Rac1

    Plasmid

    #84642

    Purpose

    Lentiviral expression of doxycycline-inducible dominant negative Rac1 and co-expression of Venus

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Dominant Negative

    Promoter

    Available since

    Dec. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pCherry.90 beta

    Plasmid

    #108223

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    mCherry-Hsp90b (HSP90AB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

    Plasmid

    #216526

    Purpose

    Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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