19,776 results

Showing: 10291 - 10320 of 19776 results

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hFerritinL

Plasmid

#122653

Purpose

Expression of hFerritinL in bacterial cells

Depositor

Trevor Douglas

Insert

light chain ferritin, human (FTL Human)

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-Tet1-CD

Plasmid

#83341

Purpose

To introduce MS2 coat protein fused Tet1-CD

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

MS2-Tet1-CD

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4 huZAP(L)

Plasmid

#45907

Depositor

Harmit Malik

Article

Kerns et al PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1

Insert

ZAP(L) isoform (ZC3HAV1 Human)

Tags

HA

Expression

Mammalian

Mutation

M202T, L388F, Q701E, T851I (reference NP_064504.2)

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pGTag-gal4-VP16-B-actin

Plasmid

#117817

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowG

Plasmid

#104620

Purpose

Expresses Shadow G in mammalian cell

Depositor

Hideji Murakoshi

Article

Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

Insert

ShadowG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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eGFP-proα2(I)

Plasmid

#119826

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag

Promoter

CMV

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAd/WT1-IRES-nAmCyan

Plasmid

#29756

Depositor

Edward McCabe

Insert

Wilms tumor 1 (WT1 Human)

Tags

AmCyan, IRES, and Nuclear localization sequence

Expression

Mammalian

Mutation

No mutations; AmCyan is nuclear localized; WT1 an…

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pdCas9- AsiA_m2.1

Plasmid

#158065

Purpose

Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA_m2.1

Expression

Bacterial

Promoter

TetO

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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IL6-GFP

Plasmid

#28088

Depositor

Jennifer Stow

Article

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Insert

IL6 (Il6 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

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BRCA1 A10.2 gRNA

Plasmid

#90549

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRCA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BRCA1 (Guide Designation A10.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1-alpha-IIb

Plasmid

#27288

Depositor

Timothy Springer

Article

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha IIb (ITGA2B Human)

Tags

His and V5

Expression

Mammalian

Mutation

n/a

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pRGD-SmR

Plasmid

#74109

Purpose

Plasmid containing streptomycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aadA

Use

Bacterial vector based on pmb1 replicon

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Article

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP-C-TEV 219

Plasmid

#100096

Purpose

SNIPer plasmid, C terminal TEV fused to FKBP

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

C-TEV

Use

Retroviral

Tags

FKBP and Myc

Expression

Mammalian

Mutation

C terminal domain

Promoter

CMV

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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Keratin 18 (pcDNA3)

Plasmid

#18064

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV5 HA-TBRII (delta Cyt)

Plasmid

#14051

Depositor

Joan Massague

Article

Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

Insert

TGF beta rec II (delta cyt) (TGFBR2 Human)

Tags

HA (PYDVPDYA)

Expression

Mammalian

Mutation

Dominant negative mutant. A stop codon was introd…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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Nsp3 -mCherry

Plasmid

#165131

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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p4947 pcDNA4c hBrd4 full-length

Plasmid

#14441

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin1 isoform1

Plasmid

#72594

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN1 (SESN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pAGM55261

Plasmid

#153210

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BLBP-mCherry

Plasmid

#63721

Purpose

Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Article

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Expression

Mammalian

Mutation

1700 bp upstream of BLBP gene cloned in CAG-mCher…

Promoter

1700bp upstream of BLBP gene

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TAK1/F

Plasmid

#44161

Depositor

Xin Lin

Article

Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

Insert

Tak1 (Map3k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pC-G418-YR

Plasmid

#61767

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

nptII gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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VP16-ER alpha

Plasmid

#11351

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER alpha (ESR1 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR Q48

Plasmid

#86428

Purpose

Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

Depositor

Michael Mancini

Article

Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

Insert

Androgen receptor (AR) (AR Human)

Tags

EGFP

Expression

Mammalian

Mutation

Poly-glutamine expansion, G130D

Promoter

CMV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-EGFP-F

Plasmid

#73014

Purpose

Retroviral expression of EGFP-F

Depositor

Michael Grusch

Insert

EGFP-F

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-wGBD

Plasmid

#26734

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

Neuronal Wiskott-Aldrich Syndrome protein (WASL Human)

Use

Reporter for cdc42

Tags

GFP

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pBG100-TCPTP

Plasmid

#33365

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

TCPTP-45 (PTPN2 Human)

Tags

6xHIS - cleavable

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only

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19,776 results